##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548000_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1560871
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.976089632006744	31.0	31.0	33.0	30.0	33.0
2	31.339114507220646	31.0	31.0	33.0	30.0	34.0
3	31.565890454752505	31.0	31.0	34.0	30.0	34.0
4	35.42864785110365	37.0	35.0	37.0	33.0	37.0
5	35.25816291032379	37.0	35.0	37.0	33.0	37.0
6	35.29468931128838	37.0	35.0	37.0	32.0	37.0
7	35.57225805335611	36.0	35.0	37.0	35.0	37.0
8	35.66149989332879	37.0	35.0	37.0	35.0	37.0
9	37.532568674797595	39.0	37.0	39.0	35.0	39.0
10	36.733945982723746	39.0	37.0	39.0	32.0	39.0
11	36.314911994649144	38.0	35.0	39.0	32.0	39.0
12	35.764874867942325	37.0	35.0	39.0	31.0	39.0
13	35.46536517111279	37.0	35.0	39.0	30.0	39.0
14	36.49438230321404	38.0	35.0	40.0	31.0	41.0
15	36.74640441138313	38.0	35.0	40.0	31.0	41.0
16	36.86850675039769	38.0	35.0	40.0	31.0	41.0
17	36.8231891040323	38.0	35.0	40.0	31.0	41.0
18	36.761305706877764	38.0	35.0	40.0	31.0	41.0
19	36.71899599646608	38.0	35.0	40.0	31.0	41.0
20	36.599743348425335	38.0	35.0	40.0	31.0	41.0
21	36.47712655305916	38.0	35.0	40.0	31.0	41.0
22	36.34422895934385	38.0	35.0	40.0	30.0	41.0
23	36.29387758501503	38.0	35.0	40.0	30.0	41.0
24	36.196893273050755	38.0	34.0	40.0	30.0	41.0
25	36.038881496292774	38.0	34.0	40.0	30.0	41.0
26	35.80767532999204	38.0	34.0	40.0	29.0	41.0
27	35.89675187763755	38.0	34.0	40.0	30.0	41.0
28	35.932126357655434	38.0	34.0	40.0	30.0	41.0
29	35.97063242253844	38.0	34.0	40.0	30.0	41.0
30	35.93943189411553	38.0	34.0	40.0	30.0	41.0
31	35.84323432237514	38.0	34.0	40.0	30.0	41.0
32	35.72447050396862	38.0	34.0	40.0	29.0	41.0
33	35.6207290672964	38.0	34.0	40.0	29.0	41.0
34	35.541547635903285	38.0	34.0	40.0	29.0	41.0
35	35.420355686024024	38.0	34.0	40.0	28.0	41.0
36	35.335962420981616	38.0	34.0	40.0	27.0	41.0
37	35.28342508765939	38.0	34.0	40.0	27.0	41.0
38	35.2390825378907	38.0	34.0	40.0	27.0	41.0
39	35.22610196486449	38.0	34.0	40.0	27.0	41.0
40	35.048753548499526	37.0	34.0	40.0	26.0	41.0
41	35.013974889660965	37.0	34.0	40.0	26.0	41.0
42	34.98702775565694	37.0	34.0	40.0	26.0	41.0
43	34.938488190247625	37.0	33.0	40.0	26.0	41.0
44	34.75762314758875	37.0	33.0	40.0	26.0	41.0
45	34.62666869971958	37.0	33.0	40.0	25.0	41.0
46	34.60873384155385	37.0	33.0	40.0	25.0	41.0
47	34.57565807808589	37.0	33.0	40.0	25.0	41.0
48	34.523625591096256	36.0	33.0	40.0	25.0	41.0
49	34.404641382920175	36.0	33.0	40.0	24.0	41.0
50	34.27204810647388	36.0	33.0	40.0	24.0	41.0
51	33.930968670697325	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	16.0
15	52.0
16	142.0
17	309.0
18	686.0
19	1352.0
20	2313.0
21	3589.0
22	5607.0
23	8368.0
24	12038.0
25	16818.0
26	21472.0
27	26288.0
28	31139.0
29	37610.0
30	46535.0
31	56955.0
32	71619.0
33	91650.0
34	140874.0
35	169979.0
36	129020.0
37	162010.0
38	229123.0
39	295275.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.34322375135421	25.60929122265709	30.2850139441376	11.762471081851094
2	28.36185693756883	27.185270275378297	31.01287678482078	13.439996002232087
3	28.079130177958334	26.894342966202846	31.18233345356535	13.844193402273476
4	25.39614100076175	29.278012084278586	30.368813310004477	14.957033604955184
5	23.02618217648992	34.09000487548298	29.199850596237614	13.68396235178948
6	20.968164569653737	43.57675938626574	25.184336181529414	10.270739862551101
7	92.81465284446952	2.969239610448269	3.360751785381367	0.8553557597008337
8	94.05389683067979	2.077878312813807	2.959245190665981	0.9089796658404186
9	89.70959163185171	5.012521854784924	3.7533530958035612	1.524533417559811
10	44.176808973963894	36.829949432079914	9.473748951707092	9.519492642249103
11	33.32933983653999	24.898534215832058	25.18696292006194	16.58516302756602
12	33.43543444653658	23.749688475216722	25.225723330115045	17.589153748131654
13	23.22741597479869	33.346061269637275	25.474686889563586	17.951835866000458
14	18.16844569474351	36.61923374833667	27.257601685212933	17.954718871706888
15	17.97932052040175	27.271952646951608	37.91722698416461	16.831499848482036
16	21.08316446394353	24.670776765024144	36.78843414990733	17.457624621125
17	21.031078160847372	23.920362413037335	27.438141909228886	27.610417516886404
18	22.503012740963218	26.04846909193649	31.52137492464143	19.927143242458857
19	27.358250617764057	27.14324245885791	25.41292650065252	20.085580422725517
20	28.405037956371793	27.282011133527373	25.993051315579567	18.319899594521264
21	24.655785135350712	27.36536203183991	28.495884669521054	19.482968163288316
22	24.673275369969716	24.386063934815883	27.707799042970237	23.232861652244164
23	21.10955998285573	29.691819503341403	27.102624111793993	22.09599640200888
24	21.502673827625728	26.248677821549638	33.72687429005985	18.52177406076479
25	20.566914242112254	28.434060213816515	31.335517156766958	19.66350838730427
26	19.723218638824093	33.72828375951632	26.02803178481758	20.520465816842005
27	19.769923331268245	32.7223710351464	28.733059938969973	18.774645694615376
28	17.96669936208694	29.781064546653756	33.864938230001066	18.387297861258233
29	18.72070145450841	27.198403968040918	33.1297717748616	20.95112280258907
30	20.25522929185051	29.926496167844746	30.468180906686076	19.350093633618666
31	25.49653366613897	27.965155352364164	26.88409227924665	19.65421870225022
32	26.06006518155568	28.187146791759215	27.609584648571218	18.14320337811389
33	25.627293991623905	28.31521631191815	26.260209844375353	19.797279852082585
34	20.48324300983233	29.2443129509101	27.802874164488927	22.469569874768638
35	20.40046871266107	28.31829151800501	30.07186372224226	21.209376047091656
36	25.91655556416898	27.387016608034877	27.37420324933963	19.322224578456513
37	20.66685843993514	31.02223053666831	28.56860048011655	19.742310543280002
38	21.217896930623994	31.503244022087667	25.67893182716573	21.599927220122613
39	20.90627604715572	30.185454147075575	28.24230830094223	20.665961504826473
40	23.63500891489431	27.4176405353165	26.951682746364046	21.99566780342514
41	19.205687081123298	27.26663510309308	28.45808526137009	25.06959255441353
42	21.90834476391707	27.589531742213165	26.482073150183457	24.020050343686314
43	21.62241466463276	28.459622864413525	26.796897373325535	23.121065097628183
44	20.58440447673126	29.686053491928543	28.70320481321006	21.026337218130134
45	19.881399551916846	32.70007579101668	25.566174270647608	21.852350386418866
46	21.927500735166454	31.065027154710418	26.65646296202569	20.35100914809744
47	21.150306463506592	28.9311544643984	27.847913120302703	22.0706259517923
48	22.424787186128768	27.171752181954815	29.198697393955044	21.20476323796137
49	21.826595535441427	26.58374715143019	29.86717031708578	21.722486996042594
50	20.231908979025174	30.842010646619737	27.71061798188319	21.215462392471895
51	19.535054466384473	31.878483231477812	26.42582250551135	22.160639796626374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1114.0
1	1287.0
2	1460.0
3	4347.0
4	7234.0
5	5744.0
6	4254.0
7	4275.5
8	4297.0
9	4505.0
10	4713.0
11	4865.5
12	5018.0
13	5138.5
14	5259.0
15	5021.5
16	4784.0
17	4822.5
18	4861.0
19	5326.5
20	5792.0
21	5934.5
22	6077.0
23	7402.5
24	8728.0
25	10708.0
26	15790.5
27	18893.0
28	22116.5
29	25340.0
30	30520.5
31	35701.0
32	39602.0
33	43503.0
34	48844.0
35	54185.0
36	60099.0
37	66013.0
38	72562.0
39	79111.0
40	92905.5
41	106700.0
42	120216.0
43	133732.0
44	135375.5
45	137019.0
46	132965.0
47	128911.0
48	124841.0
49	120771.0
50	114270.5
51	107770.0
52	100711.5
53	93653.0
54	85507.5
55	77362.0
56	71891.0
57	66420.0
58	60968.5
59	55517.0
60	50771.5
61	46026.0
62	38835.5
63	31645.0
64	26710.5
65	21776.0
66	17647.5
67	13519.0
68	10812.0
69	8105.0
70	6713.5
71	5322.0
72	4211.5
73	3101.0
74	2600.0
75	1644.0
76	1189.0
77	879.0
78	569.0
79	445.0
80	321.0
81	250.5
82	180.0
83	129.0
84	78.0
85	54.0
86	30.0
87	24.0
88	18.0
89	13.0
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1560871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.892628699785103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9919271521945	14.545798925673136
2	6.050666274847428	2.2862598263400864
3	2.1288444188214073	1.20658401493208
4	1.1968415775920713	0.9044593415164818
5	0.8378084956060863	0.7914202414505664
6	0.6734000818312172	0.7633378627465255
7	0.5466596073720821	0.7229485891075739
8	0.4569132837951004	0.6905834414992295
9	0.4351157279326854	0.7398431900342044
>10	7.923936541926316	38.616976590624226
>50	2.5122647866932635	32.2805873579985
>100	0.2438923186316542	5.938006481477485
>500	0.0010378396537502979	0.12169733600980584
>1k	6.918931025001986E-4	0.3914968005900843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3749	0.24018640874229838	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2241	0.14357368418017888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00909748467362133	0.0
2	0.0	0.0	0.0	0.03754314097705704	0.0
3	0.0	0.0	0.0	0.06118378776977726	0.0
4	0.0	0.0	0.0	0.10526174168140737	0.0
5	0.0	0.0	0.0	0.17989955608118802	0.0
6	0.0	0.0	0.0	0.30367660107721905	0.0
7	0.0	0.0	0.0	0.35396903395604123	0.0
8	0.0	0.0	0.0	0.4800524835172157	0.0
9	0.0	0.0	0.0	0.524514838189703	0.0
10	0.0	0.0	0.0	0.6026122594371989	0.0
11	0.0	0.0	0.0	0.7405480657914716	0.0
12	0.0	0.0	0.0	0.8535618894834999	0.0
13	0.0	0.0	0.0	0.9118626715468479	0.0
14	0.0	0.0	0.0	0.9414615301328553	0.0
15	0.0	0.0	0.0	0.9783640031751503	0.0
16	0.0	0.0	0.0	1.0543472202379311	0.0
17	0.0	0.0	0.0	1.1494223417566218	0.0
18	0.0	0.0	0.0	1.2623720986551739	0.0
19	0.0	0.0	0.0	1.3300266325660481	0.0
20	0.0	0.0	0.0	1.3935168249009688	0.0
21	0.0	0.0	0.0	1.4873106105501352	0.0
22	0.0	0.0	0.0	1.5928286194054473	0.0
23	0.0	0.0	0.0	1.7097505174995242	0.0
24	0.0	0.0	0.0	1.8015582325509283	0.0
25	0.0	0.0	0.0	1.8760038465702804	0.0
26	0.0	0.0	0.0	1.9589703441219677	0.0
27	0.0	0.0	0.0	2.041552440912798	0.0
28	0.0	0.0	0.0	2.124647072051438	0.0
29	0.0	0.0	0.0	2.23202301791756	0.0
30	0.0	0.0	0.0	2.3568251316092104	0.0
31	0.0	0.0	0.0	2.470223356062096	0.0
32	0.0	0.0	0.0	2.5693987523632638	0.0
33	0.0	0.0	0.0	2.6748526944250997	0.0
34	0.0	0.0	0.0	2.7938887967038917	0.0
35	0.0	0.0	0.0	2.9322090038190214	0.0
36	0.0	0.0	0.0	3.057075184304148	0.0
37	0.0	0.0	0.0	3.2040444085385658	0.0
38	0.0	0.0	0.0	3.3454398217405537	0.0
39	0.0	0.0	0.0	3.5445594158646037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACG	40	6.8175723E-9	45.000004	1
GCGTAAG	125	0.0	41.4	1
AATGCGG	215	0.0	40.813953	2
GCGAGAC	185	0.0	40.135136	21
TACGGGA	780	0.0	40.096153	4
CGACCCG	175	0.0	39.857143	32
GCTTGCG	120	0.0	39.374996	1
AGGGCGA	1410	0.0	38.776596	6
ACGGGTA	105	0.0	38.57143	5
CGAGGGA	1280	0.0	38.320316	4
GCACCGA	705	0.0	38.297874	9
CGAACGG	100	0.0	38.25	2
CGACGGT	130	0.0	38.076927	28
CGTTTTT	2670	0.0	37.92135	1
TTCGTAG	95	0.0	37.894737	1
TAGGGAT	3110	0.0	37.83762	5
ATAGGGA	2635	0.0	37.656548	4
CGCATCG	90	0.0	37.500004	21
TAGCGGG	990	0.0	37.5	3
CACGACC	205	0.0	37.317074	27
>>END_MODULE