FastQCFastQC Report
Thu 26 May 2016
SRR1547999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547999_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1720273
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65870.3829043413458213No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18250.10608781280645571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG650.044.9999969
CGACGGT1550.043.54838628
CGGTCTA1600.042.187531
CGACAAT2200.039.88636420
TACGGGA9400.039.7340434
CAACCGG403.459554E-739.3752
ACGGGTA1600.039.3755
ACGTAAG403.459554E-739.3751
CGTTTTT37800.039.1071431
TAACGGG5300.039.0566023
CGTAAGG2650.039.0566022
TTACGGG6600.038.8636363
TAGGGCG5750.038.7391325
GCGATGC3450.038.478269
ACGGGAT9500.038.3684235
AGGGATT35650.038.2468456
ATAGCGG2950.038.1355932
GACCGTA301.1401575E-437.5000049
CGTAACG301.1401575E-437.50000422
GCGGATA301.1401575E-437.50000417
Produced by FastQC (version 0.11.5)