##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547999_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1720273
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.843770727088085	31.0	31.0	31.0	30.0	33.0
2	31.19071333445331	31.0	31.0	33.0	30.0	34.0
3	31.37404295713529	31.0	31.0	34.0	30.0	34.0
4	35.25377774341631	37.0	35.0	37.0	33.0	37.0
5	35.06897219220438	35.0	35.0	37.0	32.0	37.0
6	35.10084038986835	37.0	35.0	37.0	32.0	37.0
7	35.49197249506329	35.0	35.0	37.0	35.0	37.0
8	35.58103917227091	37.0	35.0	37.0	35.0	37.0
9	37.405020017171694	39.0	37.0	39.0	35.0	39.0
10	36.53522028189712	38.0	35.0	39.0	32.0	39.0
11	36.10559137997283	38.0	35.0	39.0	31.0	39.0
12	35.57564293574334	37.0	35.0	39.0	30.0	39.0
13	35.27187022059871	37.0	35.0	39.0	30.0	39.0
14	36.27273113046592	38.0	35.0	40.0	30.0	41.0
15	36.54336666331449	38.0	35.0	40.0	30.0	41.0
16	36.65835131981959	38.0	35.0	40.0	31.0	41.0
17	36.613060252645944	38.0	35.0	40.0	31.0	41.0
18	36.51775328683296	38.0	35.0	40.0	31.0	41.0
19	36.47697720071175	38.0	35.0	40.0	30.0	41.0
20	36.34590265614818	38.0	35.0	40.0	30.0	41.0
21	36.20662999419278	38.0	34.0	40.0	30.0	41.0
22	36.08597705131685	38.0	34.0	40.0	30.0	41.0
23	36.03011498756302	38.0	34.0	40.0	30.0	41.0
24	35.91024099081948	38.0	34.0	40.0	29.0	41.0
25	35.730005644452945	38.0	34.0	40.0	29.0	41.0
26	35.503050969235694	37.0	34.0	40.0	29.0	41.0
27	35.557545226833184	37.0	34.0	40.0	29.0	41.0
28	35.594310321675685	37.0	34.0	40.0	29.0	41.0
29	35.60542658054855	37.0	34.0	40.0	29.0	41.0
30	35.54253481860147	37.0	34.0	40.0	29.0	41.0
31	35.436098805247774	37.0	34.0	40.0	28.0	41.0
32	35.29886419190442	37.0	34.0	40.0	27.0	41.0
33	35.1852607115266	37.0	34.0	40.0	27.0	41.0
34	35.056097491502804	37.0	34.0	40.0	26.0	41.0
35	34.91979877612449	37.0	33.0	40.0	25.0	41.0
36	34.840210245699375	37.0	33.0	40.0	25.0	41.0
37	34.78263973218204	37.0	33.0	40.0	25.0	41.0
38	34.70434401981546	37.0	33.0	40.0	25.0	41.0
39	34.678867830861726	37.0	33.0	40.0	24.0	41.0
40	34.49454941163408	37.0	33.0	40.0	24.0	41.0
41	34.451750972084085	37.0	33.0	40.0	24.0	41.0
42	34.39587844487474	37.0	33.0	40.0	24.0	41.0
43	34.34318971465576	37.0	33.0	40.0	24.0	41.0
44	34.195480019741055	36.0	33.0	40.0	23.0	41.0
45	34.08031399667378	36.0	33.0	40.0	23.0	41.0
46	34.017872744616696	36.0	33.0	40.0	23.0	41.0
47	33.98125413815133	36.0	33.0	40.0	23.0	41.0
48	33.90712462498685	36.0	33.0	40.0	23.0	41.0
49	33.78134691412352	36.0	32.0	40.0	23.0	41.0
50	33.67022908573232	35.0	32.0	40.0	23.0	41.0
51	33.33048766097009	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	27.0
15	64.0
16	226.0
17	556.0
18	1116.0
19	2133.0
20	3798.0
21	5707.0
22	8283.0
23	11963.0
24	16773.0
25	22540.0
26	29146.0
27	34484.0
28	40326.0
29	47614.0
30	56861.0
31	67732.0
32	82857.0
33	103837.0
34	153061.0
35	178617.0
36	144520.0
37	176052.0
38	240161.0
39	291786.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.38042624629928	26.18991288010682	28.916863776854022	13.51279709673988
2	28.071242180746893	27.819944857589462	30.10684931984633	14.001963641817316
3	28.240750160003675	26.855911823297813	31.500988505894124	13.40234951080439
4	24.878900035052574	29.520488899145658	30.572705611260538	15.027905454541227
5	23.25096074867187	33.541943633365165	29.53728855826953	13.669807059693431
6	21.591224183603416	43.44014002428685	24.94854014450032	10.02009564760942
7	91.64545394829774	3.1691481526478644	4.30600259377436	0.8793953052800341
8	92.22408303798292	2.5246574235600976	3.952279667238863	1.2989798712181149
9	87.33706801187951	5.741239907851836	5.10453864008794	1.8171534401807157
10	41.9853709265913	37.6187384211692	10.670050625685574	9.725840026553925
11	33.136891644523864	24.839022643499025	25.846944060622935	16.177141651354173
12	32.44043241973803	23.975380651791898	26.28408397969392	17.300102948776154
13	23.671591660160914	31.809079140345748	27.063437024239757	17.455892175253577
14	18.11898460302522	35.126459579380715	28.258189252519806	18.496366565074265
15	17.42508311180842	27.339381598153317	38.00204967467373	17.23348561536454
16	20.80384915650016	25.37858816594808	36.20721827291366	17.610344404638102
17	21.38776810424857	24.629404751455148	27.656656821330106	26.32617032296618
18	22.184037068535055	27.25369752359073	31.068382750877333	19.493882656996885
19	26.2256048894565	27.58678419064881	26.463706632610055	19.723904287284636
20	28.085251585068182	27.281658201924923	26.593744132471997	18.039346080534894
21	24.610221749687405	28.10571345362044	28.785954322366276	18.498110474325877
22	24.297364429948036	24.695498912091278	28.04450223888883	22.962634419071858
23	21.03590534758146	29.290699790091455	27.400302161343	22.273092700984087
24	20.82146263994145	27.690139878961073	33.2547799099329	18.233617571164576
25	20.509012232360792	28.40136420207723	31.04472371536378	20.044899850198195
26	19.979038210795615	32.90169641678966	26.619786510629417	20.49947886178531
27	19.002914072359445	32.20529532231222	29.539381249371466	19.252409355956875
28	17.861583597487144	29.966522755399872	33.671457960451626	18.500435686661362
29	18.824105243760727	27.54708119002042	32.9862178851845	20.642595681034347
30	20.64695545416338	29.397368905981786	30.330651007136655	19.62502463271818
31	24.853206438745477	28.53762164493659	26.80126933341394	19.807902582903992
32	25.763759589320994	28.62359637104111	27.166908973168795	18.4457350664691
33	24.263358199541585	29.050912267994672	26.47178674547586	20.21394278698788
34	20.279339383923364	29.31424256498823	28.498964989859164	21.907453061229234
35	19.919687165932384	29.17571804010177	29.58495541114695	21.319639382818888
36	25.095319173177742	27.905454541226888	27.082736286624275	19.916489998971095
37	20.705318283784027	31.381240070616702	28.081880027181732	19.831561618417542
38	20.743858678244674	31.586730710765092	26.09888081717262	21.57052979381761
39	20.391763400344015	30.188464272821815	28.42502323759078	20.994749089243395
40	22.80568258642669	27.782508938988173	27.25828981795331	22.153518656631828
41	18.69319578927298	27.294156218228157	28.75526151953789	25.25738647296098
42	21.579191209767288	27.9905573127056	26.983682241132655	23.446569236394456
43	21.406718584782762	27.84470836896237	28.00875209923076	22.739820947024107
44	20.172147095257557	30.26368489187472	28.301321941343033	21.262846071524695
45	19.722741681116894	32.133620652070924	26.02203254948488	22.12160511732731
46	21.609884012595675	30.834233868694096	26.97298626438943	20.582895854320796
47	20.83785538690661	28.884659586007572	27.903245589508174	22.37423943757764
48	21.459210253256312	27.8742966959314	29.56338906673534	21.103103984076945
49	21.259009471171144	26.858004514399745	29.882466329472123	22.00051968495698
50	20.056118999716904	30.55038357283989	28.270629138514643	21.122868288928558
51	19.597412736234308	31.18278319778314	26.98571680192621	22.234087264056345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1690.0
1	1904.5
2	2119.0
3	6826.5
4	11534.0
5	9008.0
6	6482.0
7	6337.0
8	6192.0
9	6711.5
10	7231.0
11	7982.5
12	8734.0
13	8897.5
14	9061.0
15	9213.0
16	9365.0
17	9109.5
18	8854.0
19	8638.5
20	8423.0
21	9458.5
22	10494.0
23	11011.0
24	11528.0
25	13054.5
26	18350.5
27	22120.0
28	25789.0
29	29458.0
30	33843.0
31	38228.0
32	43599.0
33	48970.0
34	54603.5
35	60237.0
36	64150.5
37	68064.0
38	74764.5
39	81465.0
40	95874.5
41	110284.0
42	121746.5
43	133209.0
44	137637.5
45	142066.0
46	142071.0
47	142076.0
48	137226.0
49	132376.0
50	124545.0
51	116714.0
52	109062.5
53	101411.0
54	93508.0
55	85605.0
56	79147.5
57	72690.0
58	66632.0
59	60574.0
60	54434.5
61	48295.0
62	42694.5
63	37094.0
64	30892.5
65	24691.0
66	21054.5
67	17418.0
68	14544.0
69	11670.0
70	9820.5
71	7971.0
72	6420.0
73	4869.0
74	3813.5
75	2146.0
76	1534.0
77	1311.0
78	1088.0
79	817.0
80	546.0
81	396.5
82	247.0
83	212.0
84	177.0
85	105.5
86	34.0
87	29.0
88	24.0
89	18.0
90	12.0
91	10.5
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1720273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.535151840441262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86564790105764	16.76848550703613
2	6.470630574027057	2.786920238301486
3	2.4061124497873587	1.5544799085404062
4	1.3780504785911212	1.187061052010102
5	0.9298112540036649	1.0011813268960013
6	0.6976070449327003	0.9013844182552337
7	0.5338004839022704	0.8046832131335492
8	0.4617266889894631	0.795468348493783
9	0.4081176316580661	0.7909987649865958
>10	6.580129973561253	35.84849441058555
>50	2.005438476982518	29.480535443543815
>100	0.25989856348919343	7.168845615321638
>500	0.002202530194713834	0.33445363612076984
>1k	5.506325486784585E-4	0.18646635093065433
>5k	2.7531627433922924E-4	0.3905417658442671
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6587	0.3829043413458213	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1825	0.10608781280645571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015172010489032845	0.0
2	0.0	0.0	0.0	0.058886002396131315	0.0
3	0.0	0.0	0.0	0.0895788052245196	0.0
4	0.0	0.0	0.0	0.15038310779742517	0.0
5	0.0	0.0	0.0	0.2678644610477523	0.0
6	0.0	0.0	0.0	0.46806524313292136	0.0
7	0.0	0.0	0.0	0.5654335096813122	0.0
8	0.0	0.0	0.0	0.796617746136805	0.0
9	0.0	0.0	0.0	0.8853245967355181	0.0
10	0.0	0.0	0.0	1.0270462885832656	0.0
11	0.0	0.0	0.0	1.2434654267084353	0.0
12	0.0	0.0	0.0	1.4097762390039255	0.0
13	0.0	0.0	0.0	1.4958090954168322	0.0
14	0.0	0.0	0.0	1.543301557369092	0.0
15	0.0	0.0	0.0	1.5963745289265134	0.0
16	0.0	0.0	0.0	1.6949635319510334	0.0
17	0.0	0.0	0.0	1.8147700975368444	0.0
18	0.0	0.0	0.0	1.954166577049108	0.0
19	0.0	0.0	0.0	2.0421177336387886	0.0
20	0.0	0.0	0.0	2.134835575516212	0.0
21	0.0	0.0	0.0	2.2570254837458936	0.0
22	0.0	0.0	0.0	2.4028162971807383	0.0
23	0.0	0.0	0.0	2.5633722089459057	0.0
24	0.0	0.0	0.0	2.686201550567846	0.0
25	0.0	0.0	0.0	2.788743414562689	0.0
26	0.0	0.0	0.0	2.899539782348499	0.0
27	0.0	0.0	0.0	3.010859322909794	0.0
28	0.0	0.0	0.0	3.1197955208272177	0.0
29	0.0	0.0	0.0	3.2525070148749644	0.0
30	0.0	0.0	0.0	3.413004796331745	0.0
31	0.0	0.0	0.0	3.5554240518801374	0.0
32	0.0	0.0	0.0	3.68261316663111	0.0
33	0.0	0.0	0.0	3.817359221472406	0.0
34	0.0	0.0	0.0	3.954895531116282	0.0
35	0.0	0.0	0.0	4.116497788432417	0.0
36	0.0	0.0	0.0	4.260544692615649	0.0
37	0.0	0.0	0.0	4.421333125614365	0.0
38	0.0	0.0	0.0	4.589445977469855	0.0
39	0.0	0.0	0.0	4.825222508287928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	65	0.0	44.999996	9
CGACGGT	155	0.0	43.548386	28
CGGTCTA	160	0.0	42.1875	31
CGACAAT	220	0.0	39.886364	20
TACGGGA	940	0.0	39.734043	4
CAACCGG	40	3.459554E-7	39.375	2
ACGGGTA	160	0.0	39.375	5
ACGTAAG	40	3.459554E-7	39.375	1
CGTTTTT	3780	0.0	39.107143	1
TAACGGG	530	0.0	39.056602	3
CGTAAGG	265	0.0	39.056602	2
TTACGGG	660	0.0	38.863636	3
TAGGGCG	575	0.0	38.739132	5
GCGATGC	345	0.0	38.47826	9
ACGGGAT	950	0.0	38.368423	5
AGGGATT	3565	0.0	38.246845	6
ATAGCGG	295	0.0	38.135593	2
GACCGTA	30	1.1401575E-4	37.500004	9
CGTAACG	30	1.1401575E-4	37.500004	22
GCGGATA	30	1.1401575E-4	37.500004	17
>>END_MODULE