FastQCFastQC Report
Thu 26 May 2016
SRR1547998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547998_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2576328
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140790.5464754487782612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG3450.042.3913042
GTTTACG603.6379788E-1241.2499961
CGTTTTT95000.040.9736861
GGGCGAT41700.038.2014357
AGGGCGA23250.038.032266
TAGGGAT43450.038.0092055
CGACGGT1450.037.2413828
TAGGGAC30700.037.01145
ATAGGGA39250.036.91724
AGGGATT49100.036.8431786
ACGGGAT13600.036.727945
GGCGATT10550.036.6824658
AAGGGAT45100.036.6186265
CGTAAGG4550.036.593412
TAAGGGA37900.036.4511874
TACGGGA11050.036.4479644
AAGGGCG10750.036.4186065
TATGGGA32950.036.3960534
AGGGATG50950.036.2119756
TTAGGGA39800.036.1243744
Produced by FastQC (version 0.11.5)