##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547998_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2576328
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86660821137681	31.0	31.0	31.0	30.0	33.0
2	31.204449899236433	31.0	31.0	33.0	30.0	34.0
3	31.3395526501284	31.0	31.0	34.0	30.0	34.0
4	35.25568755220608	37.0	35.0	37.0	33.0	37.0
5	35.10089941963911	37.0	35.0	37.0	32.0	37.0
6	35.14810847066057	37.0	35.0	37.0	32.0	37.0
7	35.503940492049146	35.0	35.0	37.0	35.0	37.0
8	35.58150398551737	37.0	35.0	37.0	35.0	37.0
9	37.397320915659805	39.0	37.0	39.0	35.0	39.0
10	36.55950057601361	39.0	35.0	39.0	32.0	39.0
11	36.17884873354635	38.0	35.0	39.0	31.0	39.0
12	35.76949441220217	37.0	35.0	39.0	30.0	39.0
13	35.61033028403216	37.0	35.0	39.0	30.0	39.0
14	36.59429001276235	38.0	35.0	40.0	30.0	41.0
15	36.763566207408374	38.0	35.0	40.0	31.0	41.0
16	36.84947025378756	38.0	35.0	40.0	31.0	41.0
17	36.773455088016746	38.0	35.0	40.0	31.0	41.0
18	36.67052098956344	38.0	35.0	40.0	31.0	41.0
19	36.60187639151537	38.0	35.0	40.0	30.0	41.0
20	36.455080253756506	38.0	35.0	40.0	30.0	41.0
21	36.351097375799974	38.0	35.0	40.0	30.0	41.0
22	36.24325202381063	38.0	34.0	40.0	30.0	41.0
23	36.15274646706475	38.0	34.0	40.0	30.0	41.0
24	36.04001082160346	38.0	34.0	40.0	30.0	41.0
25	35.86134218934856	38.0	34.0	40.0	29.0	41.0
26	35.62078974416301	38.0	34.0	40.0	29.0	41.0
27	35.71018402936272	38.0	34.0	40.0	29.0	41.0
28	35.727731096351086	38.0	34.0	40.0	29.0	41.0
29	35.68636640986707	38.0	34.0	40.0	29.0	41.0
30	35.59178295620744	38.0	34.0	40.0	28.0	41.0
31	35.504191624668906	38.0	34.0	40.0	28.0	41.0
32	35.3603628885763	38.0	34.0	40.0	27.0	41.0
33	35.19122371064554	38.0	34.0	40.0	26.0	41.0
34	34.987133237693335	38.0	34.0	40.0	25.0	41.0
35	34.830027853596285	38.0	33.0	40.0	24.0	41.0
36	34.7594669622812	38.0	33.0	40.0	24.0	41.0
37	34.70377413124416	38.0	33.0	40.0	24.0	41.0
38	34.62234544669778	37.0	33.0	40.0	24.0	41.0
39	34.558722724746225	37.0	33.0	40.0	24.0	41.0
40	34.38861783126993	37.0	33.0	40.0	23.0	41.0
41	34.314191748876695	37.0	33.0	40.0	23.0	41.0
42	34.263366310500835	37.0	33.0	40.0	23.0	41.0
43	34.21850168146292	37.0	33.0	40.0	23.0	41.0
44	34.06842180032977	37.0	33.0	40.0	23.0	41.0
45	33.95339917898653	37.0	33.0	40.0	23.0	41.0
46	33.8985987032707	36.0	33.0	40.0	23.0	41.0
47	33.8502764399564	36.0	33.0	40.0	23.0	41.0
48	33.7426632012694	36.0	32.0	40.0	22.0	41.0
49	33.583409022453665	36.0	32.0	40.0	22.0	41.0
50	33.4803600317972	36.0	32.0	40.0	22.0	41.0
51	33.155721631717704	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	7.0
13	12.0
14	43.0
15	136.0
16	345.0
17	842.0
18	1765.0
19	3378.0
20	5809.0
21	9064.0
22	13133.0
23	18775.0
24	26387.0
25	36772.0
26	47129.0
27	54854.0
28	61878.0
29	70426.0
30	82845.0
31	99191.0
32	120210.0
33	148413.0
34	211005.0
35	238298.0
36	224607.0
37	277455.0
38	376287.0
39	447195.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.754729987796587	25.258546272058524	28.35492996233399	14.6317937778109
2	30.634104042652954	26.582562468753977	29.402195683158354	13.381137805434712
3	28.740633956545906	26.585822923168166	31.18186038423679	13.491682736049137
4	25.85823699466838	28.76916293267006	30.793051195344688	14.579548877316864
5	23.122133517160858	33.00220313562559	29.704253495672912	14.171409851540643
6	21.96991221614639	41.76265599721775	26.35743585444089	9.909995932194969
7	90.12136653407485	2.886162010427244	5.872233659689295	1.1202377958086083
8	90.65227719451872	2.4144829385078297	5.592183914470518	1.3410559525029422
9	85.48468983762938	5.508925882108179	6.938479882996264	2.0679043972661866
10	40.17500877217497	36.59619427339997	12.821504094199184	10.407292860225873
11	30.889739194698812	25.32464810381287	26.549375700609552	17.23623700087877
12	30.244130405755786	23.4290820112967	27.82378641228912	18.503001170658393
13	24.837016094224026	26.695863259647062	28.79008418182778	19.67703646430113
14	20.047796709114678	29.134760791327814	30.6571989280868	20.160243571470712
15	20.21221676743023	26.366402104079917	34.915740542353305	18.505640586136547
16	24.0175940330579	24.98676410767573	32.626008800121724	18.369633059144643
17	23.805703311069088	24.823236792830727	28.8334016476163	22.53765824848389
18	24.25087178340646	25.581175999329275	30.321372123425277	19.846580093838984
19	26.601154821901556	26.88178679112287	27.008944513276262	19.508113873699312
20	27.764050229629145	26.954487161572594	27.332661058685076	17.948801550113185
21	26.31116845370621	26.20326293856993	29.349019224260264	18.136549383463596
22	25.37149772855009	23.3843672079021	29.174662542968132	22.069472520579676
23	23.039884673069576	27.109087041712083	28.908392099142656	20.942636186075685
24	23.101445157604157	25.851133861837468	31.50449011150754	19.542930869050835
25	23.178880949941156	27.250101695125778	28.9435196139622	20.62749774097087
26	21.80793749864148	29.726028673367676	27.25254703593642	21.21348679205443
27	20.594815566961973	29.155099816482995	29.625497995596834	20.6245866209582
28	20.42725149903273	28.219077695076095	31.26445856272959	20.089212243161587
29	21.296667194549762	26.853684779267233	31.091538033977038	20.758109992205963
30	21.89923022223878	27.04729366757649	30.286399868339746	20.767076241844983
31	24.592870162494837	26.801633953440714	27.995930642371626	20.609565241692827
32	25.71524277964607	26.96554941762074	27.765408752301724	19.55379905043147
33	25.084111960899392	27.221029309932586	27.23601187426446	20.458846854903566
34	22.020177555031815	27.34845873662049	29.44493092494434	21.186432783403355
35	21.930437428774596	27.94166736533547	29.019247549225096	21.108647656664832
36	24.584524951791852	27.911741051605233	27.0251303405467	20.478603656056215
37	22.229622936209985	29.193138451315203	27.62850848183927	20.94873013063554
38	22.606515940516893	29.61435810968169	26.55911824891862	21.220007700882807
39	22.14457941690654	28.66020941432923	26.95728183678476	22.23792933197947
40	24.04177573662981	26.922154321965213	27.55611863085756	21.479951310547417
41	20.79471247449859	26.851744032592123	28.382061600852065	23.971481892057223
42	22.215882449750186	27.299435475607144	27.1042739899578	23.38040808468487
43	21.877687934145033	26.924483217975354	27.97799814309358	23.219830704786038
44	21.959276924366772	27.71921898143404	28.112297813011388	22.2092062811878
45	21.364981477513734	28.550013818116327	27.453491946677598	22.63151275769234
46	21.81624389441096	28.691494250732045	27.339376042180962	22.152885812676026
47	21.38660139547449	27.80779465968619	28.37849062696986	22.427113317869463
48	22.25170863337277	26.990390975062184	29.078362692949035	21.679537698616013
49	22.42462916212532	26.609189513136528	29.046379187743177	21.919802136994978
50	20.917484109166224	28.841087004449744	28.31013752907239	21.931291357311647
51	20.501310392155037	28.980316170922336	28.06556463307467	22.452808803847958
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2033.0
1	2468.5
2	2904.0
3	14375.0
4	25846.0
5	19968.5
6	14091.0
7	13164.5
8	12238.0
9	12556.0
10	12874.0
11	12889.0
12	12904.0
13	12803.0
14	12702.0
15	12688.0
16	12674.0
17	12278.5
18	11883.0
19	11753.5
20	11624.0
21	12125.0
22	12626.0
23	14405.5
24	16185.0
25	18242.0
26	23295.0
27	26291.0
28	32489.5
29	38688.0
30	43491.0
31	48294.0
32	57235.5
33	66177.0
34	73275.5
35	80374.0
36	89004.5
37	97635.0
38	107247.0
39	116859.0
40	128385.0
41	139911.0
42	153833.5
43	167756.0
44	172631.5
45	177507.0
46	179451.5
47	181396.0
48	185866.0
49	190336.0
50	181490.5
51	172645.0
52	165113.0
53	157581.0
54	148821.5
55	140062.0
56	133483.5
57	126905.0
58	121160.5
59	115416.0
60	106266.5
61	97117.0
62	87535.5
63	77954.0
64	67920.0
65	57886.0
66	49448.5
67	41011.0
68	34309.0
69	27607.0
70	23442.5
71	19278.0
72	15950.5
73	12623.0
74	10564.0
75	6397.5
76	4290.0
77	3331.5
78	2373.0
79	1913.0
80	1453.0
81	1127.5
82	802.0
83	586.5
84	371.0
85	269.0
86	167.0
87	131.5
88	96.0
89	71.0
90	46.0
91	32.5
92	19.0
93	14.5
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2576328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.52135866002588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18852173831563	15.966377847874172
2	6.159416222211696	2.6511801130919914
3	2.500747323813664	1.614584402216812
4	1.6006919618584332	1.377962632615032
5	1.1852740851989272	1.2754354349000092
6	0.9248848773997833	1.194286749945288
7	0.7804227335665467	1.1757030288866428
8	0.6893784733092777	1.1869089101272035
9	0.6531748329682359	1.265148886321071
>10	9.949695983905709	51.657084592696926
>50	1.3086632823021018	17.84960915983895
>100	0.056726658545979636	1.82264336517819
>500	0.0014780471409499205	0.2124482500355885
>1k	7.390235704749602E-4	0.19081773384255837
>5k	0.0	0.0
>10k+	1.8475589261874006E-4	0.5598088924295435
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14079	0.5464754487782612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01696212594048584	0.0
2	0.0	0.0	0.0	0.05104163755546654	0.0
3	0.0	0.0	0.0	0.08698426597855552	0.0
4	0.0	0.0	0.0	0.15425054573796504	0.0
5	0.0	0.0	0.0	0.26852170996860647	0.0
6	0.0	0.0	0.0	0.535684897264634	0.0
7	0.0	0.0	0.0	0.6613288370114364	0.0
8	0.0	0.0	0.0	0.9182836967963707	0.0
9	0.0	0.0	0.0	1.0626752494247627	0.0
10	0.0	0.0	0.0	1.2506947873096903	0.0
11	0.0	0.0	0.0	1.4408491465372422	0.0
12	0.0	0.0	0.0	1.5869097413062312	0.0
13	0.0	0.0	0.0	1.6736999326172755	0.0
14	0.0	0.0	0.0	1.7347170080828218	0.0
15	0.0	0.0	0.0	1.7856422008377815	0.0
16	0.0	0.0	0.0	1.865018739849895	0.0
17	0.0	0.0	0.0	1.9515760415599257	0.0
18	0.0	0.0	0.0	2.0492732291851037	0.0
19	0.0	0.0	0.0	2.12337093724091	0.0
20	0.0	0.0	0.0	2.2012336938464356	0.0
21	3.881493350225592E-5	0.0	0.0	2.2953210926559042	0.0
22	3.881493350225592E-5	0.0	0.0	2.403614757127198	0.0
23	3.881493350225592E-5	0.0	0.0	2.5221555640430875	0.0
24	3.881493350225592E-5	0.0	0.0	2.6204349756707996	0.0
25	3.881493350225592E-5	0.0	0.0	2.7126204427386575	0.0
26	3.881493350225592E-5	0.0	0.0	2.8066690266146237	0.0
27	3.881493350225592E-5	0.0	0.0	2.9049096233088334	0.0
28	3.881493350225592E-5	0.0	0.0	3.002218661598989	0.0
29	3.881493350225592E-5	0.0	0.0	3.1147043388885267	0.0
30	3.881493350225592E-5	0.0	0.0	3.2403870935688315	0.0
31	3.881493350225592E-5	0.0	0.0	3.358500936216196	0.0
32	3.881493350225592E-5	0.0	0.0	3.4753727009914885	0.0
33	3.881493350225592E-5	0.0	0.0	3.5881688977490445	0.0
34	3.881493350225592E-5	0.0	0.0	3.713812837495847	0.0
35	3.881493350225592E-5	0.0	0.0	3.8483453970146657	0.0
36	3.881493350225592E-5	0.0	0.0	3.97849186904773	0.0
37	3.881493350225592E-5	0.0	0.0	4.121059119801516	0.0
38	3.881493350225592E-5	0.0	0.0	4.284237100245	0.0
39	3.881493350225592E-5	0.0	0.0	4.513827431910844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	345	0.0	42.391304	2
GTTTACG	60	3.6379788E-12	41.249996	1
CGTTTTT	9500	0.0	40.973686	1
GGGCGAT	4170	0.0	38.201435	7
AGGGCGA	2325	0.0	38.03226	6
TAGGGAT	4345	0.0	38.009205	5
CGACGGT	145	0.0	37.24138	28
TAGGGAC	3070	0.0	37.0114	5
ATAGGGA	3925	0.0	36.9172	4
AGGGATT	4910	0.0	36.843178	6
ACGGGAT	1360	0.0	36.72794	5
GGCGATT	1055	0.0	36.682465	8
AAGGGAT	4510	0.0	36.618626	5
CGTAAGG	455	0.0	36.59341	2
TAAGGGA	3790	0.0	36.451187	4
TACGGGA	1105	0.0	36.447964	4
AAGGGCG	1075	0.0	36.418606	5
TATGGGA	3295	0.0	36.396053	4
AGGGATG	5095	0.0	36.211975	6
TTAGGGA	3980	0.0	36.124374	4
>>END_MODULE