FastQCFastQC Report
Thu 26 May 2016
SRR1547997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547997_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2264307
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51310.22660354801711957No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33840.14944969917948406No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24900.10996742049554234No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA207.034738E-445.039
ACCGTAC207.034738E-445.014
TACGTCC207.034738E-445.028
CGAACTA451.9297659E-840.00000432
CGGTCTA2800.039.37531
TATGGGC7000.038.5714264
CGGTAGT4800.038.43750412
ACGGGAT8050.038.2919245
CGCATCG1650.038.18181621
TTAATCG659.094947E-1238.07692720
CTCACGA2900.038.01724224
TACGGGA6800.037.720594
ATAACGC1850.037.702711
GACGTAG900.037.5000041
CAATACG900.037.5000041
GGGCGAT34350.037.401757
TAGGGAC19450.037.1336755
AGGGCGA18450.036.829276
CGACGGT2950.036.6101728
CGCATGG2600.036.3461572