##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547997_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2264307
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.822599144020664	31.0	31.0	31.0	30.0	33.0
2	31.174429085808594	31.0	31.0	33.0	30.0	34.0
3	31.369586809562485	31.0	31.0	34.0	30.0	34.0
4	35.23127561766139	35.0	35.0	37.0	33.0	37.0
5	35.0386078389547	35.0	35.0	37.0	32.0	37.0
6	35.07072627519148	37.0	35.0	37.0	32.0	37.0
7	35.45778907188822	35.0	35.0	37.0	35.0	37.0
8	35.529373887904775	37.0	35.0	37.0	35.0	37.0
9	37.339330311658266	39.0	37.0	39.0	35.0	39.0
10	36.459573282244854	38.0	35.0	39.0	32.0	39.0
11	36.065135602195284	38.0	35.0	39.0	31.0	39.0
12	35.321905554326335	37.0	35.0	39.0	30.0	39.0
13	34.9189526861861	37.0	34.0	39.0	27.0	39.0
14	35.87814108245922	38.0	34.0	40.0	27.0	41.0
15	36.18098296741564	38.0	34.0	40.0	30.0	41.0
16	36.36387689478503	38.0	35.0	40.0	31.0	41.0
17	36.29576201460314	38.0	34.0	40.0	30.0	41.0
18	36.22587308169784	38.0	35.0	40.0	30.0	41.0
19	36.15487034222833	38.0	34.0	40.0	30.0	41.0
20	36.01477273178946	38.0	34.0	40.0	30.0	41.0
21	35.859779614690055	37.0	34.0	40.0	29.0	41.0
22	35.7238620911387	37.0	34.0	40.0	29.0	41.0
23	35.68604654757504	37.0	34.0	40.0	29.0	41.0
24	35.54601694911511	37.0	34.0	40.0	29.0	41.0
25	35.36842883937558	37.0	34.0	40.0	28.0	41.0
26	35.09849282804849	37.0	34.0	40.0	27.0	41.0
27	35.153314457800995	37.0	34.0	40.0	27.0	41.0
28	35.209233995213545	37.0	34.0	40.0	27.0	41.0
29	35.17627247541963	37.0	34.0	40.0	27.0	41.0
30	35.13046994069267	37.0	34.0	40.0	27.0	41.0
31	35.003395740948555	37.0	33.0	40.0	27.0	41.0
32	34.86038686450203	36.0	33.0	40.0	26.0	41.0
33	34.73110713344083	36.0	33.0	40.0	26.0	41.0
34	34.611906424349705	36.0	33.0	40.0	25.0	41.0
35	34.49034958598812	36.0	33.0	40.0	24.0	41.0
36	34.406780087682456	36.0	33.0	40.0	24.0	41.0
37	34.322543277037965	36.0	33.0	40.0	24.0	41.0
38	34.26648771566753	36.0	33.0	40.0	24.0	41.0
39	34.23435249725413	36.0	33.0	40.0	24.0	41.0
40	33.989939085115225	36.0	33.0	40.0	23.0	41.0
41	33.94118907021	36.0	33.0	40.0	23.0	41.0
42	33.87776613330259	36.0	33.0	40.0	23.0	41.0
43	33.81922990124572	35.0	33.0	40.0	23.0	41.0
44	33.66179497744785	35.0	32.0	40.0	23.0	41.0
45	33.5266626831079	35.0	32.0	40.0	23.0	41.0
46	33.47344860922128	35.0	32.0	39.0	23.0	41.0
47	33.40917728912201	35.0	32.0	39.0	23.0	41.0
48	33.3159152005448	35.0	32.0	39.0	22.0	41.0
49	33.182550776021095	35.0	32.0	39.0	22.0	41.0
50	33.03979098240654	35.0	31.0	39.0	21.0	41.0
51	32.668832009087104	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	1.0
13	11.0
14	44.0
15	126.0
16	366.0
17	918.0
18	1938.0
19	3450.0
20	5889.0
21	8889.0
22	12837.0
23	18257.0
24	25274.0
25	33599.0
26	42081.0
27	49755.0
28	58335.0
29	68464.0
30	81237.0
31	96943.0
32	117117.0
33	146499.0
34	220924.0
35	252911.0
36	182348.0
37	219523.0
38	286673.0
39	329867.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.15195642640331	25.865397227496096	28.821312657691735	13.16133368840886
2	30.247444361564046	26.636803225004385	29.413458510705482	13.702293902726087
3	29.528681402301014	26.807583953942643	30.00745923587217	13.656275407884177
4	25.95633012661269	28.901337142004152	29.12016789242801	16.022164838955142
5	22.49995252410561	33.541211505330324	28.601775289304854	15.35706068125921
6	22.34507069933538	41.32964302102145	26.163810826005484	10.161475453637692
7	90.13799807181624	3.6565271405335054	4.819178671443404	1.386296116206857
8	90.62737517483274	2.8698405295748324	4.292571634500092	2.2102126610923345
9	85.47060977155483	5.605114500816365	6.405271016695174	2.519004710933632
10	45.29363730271558	30.768486782048548	12.096195436396213	11.841680478839663
11	39.36250693920921	23.195220436098108	21.68380877681339	15.758463847879284
12	36.071610430917715	21.778230602122413	24.71807047365927	17.432088493300597
13	24.063433094540628	32.800587552836255	25.878072187207827	17.25790716541529
14	18.987001320933956	35.50662520585768	27.58296467749294	17.923408795715424
15	17.53649129733733	25.781883816991247	39.21946096531963	17.462163920351788
16	20.586431080237794	22.410388697292372	38.13126930226334	18.871910920206492
17	21.101908884263484	23.01410541945063	26.659105854462318	29.22487984182357
18	23.08516468835719	25.11841371333481	30.954548124437192	20.841873473870816
19	28.330036518899604	26.260484996071646	24.588759386425956	20.820719098602797
20	29.668856740715814	25.707556440005707	24.485239854843005	20.138346964435478
21	25.013657600316563	27.56772822766524	27.622093647195367	19.796520524822828
22	24.637913498478785	23.47936918447896	26.383833994241947	25.49888332280031
23	21.870665064410435	28.712581818631485	25.462006697855017	23.954746419103063
24	21.968619979534576	25.769871311619845	33.414991871685245	18.846516837160333
25	21.535639822691888	26.118543112749286	30.47161891033327	21.874198154225553
26	20.07201320315664	32.135880867744525	25.380215668635035	22.4118902604638
27	20.25515974644781	31.161498860357717	28.12975449000511	20.453586903189365
28	18.52853875379973	28.148921502252122	33.049140421329795	20.273399322618353
29	19.203226417619167	25.452246537240754	32.796480335926184	22.5480467092139
30	21.38080216154435	27.959547888161808	29.86202842635738	20.797621523936463
31	27.129713417836008	26.72367307083359	25.308979745237725	20.837633766092672
32	27.208501320713136	25.80798451800043	26.105868153037555	20.87764600824888
33	26.53266540270379	26.537258419463438	25.574844753825342	21.355231424007435
34	21.23369313436738	26.75644247886881	27.67919721133221	24.3306671754316
35	20.16692082831524	26.28808726025225	30.034443209335134	23.510548702097374
36	27.882659021060306	25.325099467519202	25.80065335663406	20.99158815478643
37	21.396745229335068	29.574523242652166	28.067483782013657	20.961247745999106
38	21.796426014670274	30.234681074606932	24.496854887610205	23.47203802311259
39	21.492182817965936	28.72817157744069	26.923027663651617	22.856617940941753
40	23.977843993769397	25.49239127026503	26.941885530539807	23.587879205425764
41	19.538958277300736	24.21257364836129	28.422294326696868	27.826173747641107
42	22.73940768632522	25.986228899173124	25.511160809907846	25.76320260459381
43	21.92215984846578	25.197466597948072	27.72252172519009	25.157851828396062
44	21.020736145761155	27.436694759147056	28.338825079814704	23.20374401527708
45	19.89734607542175	31.320664556528776	25.36228523782332	23.419704130226158
46	23.419527475735403	27.848520540721733	26.702430368320197	22.029521615222674
47	22.07253698372173	26.85152675851817	27.363868945332943	23.712067312427155
48	22.744751484670587	25.13894979788518	29.609324177331075	22.506974540113156
49	22.31587854473797	24.294320513958574	30.14962193730797	23.240179003995483
50	20.88537464221945	28.52104418702941	28.161773116454615	22.431808054296525
51	19.81612917329673	29.784874577519744	26.10003855484261	24.298957694340917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2053.0
1	2693.0
2	3333.0
3	6931.5
4	10530.0
5	8493.5
6	6457.0
7	6422.5
8	6388.0
9	6973.0
10	7558.0
11	7871.0
12	8184.0
13	8311.0
14	8438.0
15	8813.5
16	9189.0
17	8837.5
18	8486.0
19	9002.0
20	9518.0
21	9514.0
22	9510.0
23	10064.0
24	10618.0
25	11827.5
26	15564.0
27	18091.0
28	23132.5
29	28174.0
30	31176.0
31	34178.0
32	42263.5
33	50349.0
34	52600.5
35	54852.0
36	60782.5
37	66713.0
38	78227.0
39	89741.0
40	111043.5
41	132346.0
42	152770.0
43	173194.0
44	180282.0
45	187370.0
46	191688.5
47	196007.0
48	193366.5
49	190726.0
50	179051.5
51	167377.0
52	154330.5
53	141284.0
54	128430.0
55	115576.0
56	110219.5
57	104863.0
58	96952.5
59	89042.0
60	85225.5
61	81409.0
62	74073.5
63	66738.0
64	59270.0
65	51802.0
66	44018.5
67	36235.0
68	30385.5
69	24536.0
70	20876.0
71	17216.0
72	15362.0
73	13508.0
74	11211.0
75	6926.0
76	4938.0
77	4022.5
78	3107.0
79	2242.0
80	1377.0
81	1030.5
82	684.0
83	493.0
84	302.0
85	265.0
86	228.0
87	150.5
88	73.0
89	53.5
90	34.0
91	25.5
92	17.0
93	11.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2264307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.865926328341477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94984814195337	15.88277793150134
2	7.268477427721725	2.8879007419666545
3	2.2989433268887187	1.3701191629501064
4	1.169211083292591	0.9290984497188378
5	0.7234661992976141	0.7186163108145807
6	0.5255673275643209	0.6264529085985666
7	0.39412406087120866	0.5480747690245942
8	0.3124295060164026	0.4965361239457579
9	0.24751235939643973	0.4425356067411285
>10	4.093888882649201	21.208126817171593
>50	2.170251568713726	31.081875269782422
>100	0.8392277816369158	22.177650876225616
>500	0.004549892901850107	0.6021637713597667
>1k	0.0022749464509250533	0.7961812638880976
>5k	2.2749464509250528E-4	0.23188999631093993
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5131	0.22660354801711957	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3384	0.14944969917948406	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2490	0.10996742049554234	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03881982434360712	0.0
2	0.0	0.0	0.0	0.17612452728362366	0.0
3	0.0	0.0	0.0	0.27200375214138367	0.0
4	0.0	0.0	0.0	0.5773510394129417	0.0
5	0.0	0.0	0.0	0.9819781504893108	0.0
6	0.0	0.0	0.0	1.5976190507735921	0.0
7	0.0	0.0	0.0	1.8355284861990888	0.0
8	0.0	0.0	0.0	2.4052392188868383	0.0
9	0.0	0.0	0.0	2.5689096045721715	0.0
10	0.0	0.0	0.0	2.855664006691672	0.0
11	0.0	0.0	0.0	3.278486530315898	0.0
12	0.0	0.0	0.0	3.602073393758002	0.0
13	0.0	0.0	0.0	3.7523180381458876	0.0
14	0.0	0.0	0.0	3.8219199075037085	0.0
15	0.0	0.0	0.0	3.9081714626152726	0.0
16	0.0	0.0	0.0	4.07767144649555	0.0
17	0.0	0.0	0.0	4.262672861939658	0.0
18	0.0	0.0	0.0	4.496342589587012	0.0
19	0.0	0.0	0.0	4.626669440142171	0.0
20	0.0	0.0	0.0	4.765343215385546	0.0
21	0.0	0.0	0.0	4.958559064649802	0.0
22	0.0	0.0	0.0	5.161844219887144	0.0
23	0.0	0.0	0.0	5.383722260276544	0.0
24	0.0	0.0	0.0	5.5506607540408615	0.0
25	0.0	0.0	0.0	5.691896019400196	0.0
26	0.0	0.0	0.0	5.827566668300721	0.0
27	0.0	0.0	0.0	5.959395082027305	0.0
28	0.0	0.0	0.0	6.103103510257222	0.0
29	0.0	0.0	0.0	6.261120952238367	0.0
30	0.0	0.0	0.0	6.459503945357233	0.0
31	0.0	0.0	0.0	6.635319327282033	0.0
32	0.0	0.0	0.0	6.798945549344678	0.0
33	0.0	0.0	0.0	6.966237352090507	0.0
34	0.0	0.0	0.0	7.134324100044738	0.0
35	0.0	0.0	0.0	7.344233798685425	0.0
36	0.0	0.0	0.0	7.514926200378306	0.0
37	0.0	0.0	0.0	7.704167323600554	0.0
38	0.0	0.0	0.0	7.897074027505988	0.0
39	0.0	0.0	0.0	8.151765639553293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCA	20	7.034738E-4	45.0	39
ACCGTAC	20	7.034738E-4	45.0	14
TACGTCC	20	7.034738E-4	45.0	28
CGAACTA	45	1.9297659E-8	40.000004	32
CGGTCTA	280	0.0	39.375	31
TATGGGC	700	0.0	38.571426	4
CGGTAGT	480	0.0	38.437504	12
ACGGGAT	805	0.0	38.291924	5
CGCATCG	165	0.0	38.181816	21
TTAATCG	65	9.094947E-12	38.076927	20
CTCACGA	290	0.0	38.017242	24
TACGGGA	680	0.0	37.72059	4
ATAACGC	185	0.0	37.7027	11
GACGTAG	90	0.0	37.500004	1
CAATACG	90	0.0	37.500004	1
GGGCGAT	3435	0.0	37.40175	7
TAGGGAC	1945	0.0	37.133675	5
AGGGCGA	1845	0.0	36.82927	6
CGACGGT	295	0.0	36.61017	28
CGCATGG	260	0.0	36.346157	2
>>END_MODULE