FastQCFastQC Report
Thu 26 May 2016
SRR1547996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547996_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1644138
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46280.28148488752160705No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32250.19615141794666874No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17050.1037017573950605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT207.0341723E-445.00000414
TCGATTG406.8175723E-945.0000041
ACTATCG253.891541E-545.022
TCGTATC253.891541E-545.036
TAGTACG551.8189894E-1245.01
ATTACGA302.1660744E-644.99999617
CCCCGTA302.1660744E-644.99999640
GCGTAAG1050.042.8571431
TATTACG650.041.538461
TACGGGA6200.039.9193534
TAAGGGA18300.039.713114
ATATGCG800.039.3750041
CGTTTTT30900.039.320391
CGTAAGG2700.039.1666682
CGAAAGG2650.039.0566022
ACGTAAG750.039.01
TCACGAC2950.038.89830425
CGACAGG1450.038.7931022
CGCAGTA3100.038.4677437
CGGGTAT1000.038.256
Produced by FastQC (version 0.11.5)