##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547996_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1644138
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99342147678601	31.0	31.0	33.0	30.0	33.0
2	31.34327775405714	31.0	31.0	33.0	30.0	34.0
3	31.538291797890444	31.0	31.0	34.0	30.0	34.0
4	35.414150150413164	37.0	35.0	37.0	33.0	37.0
5	35.256103806371485	37.0	35.0	37.0	33.0	37.0
6	35.286211376417306	37.0	35.0	37.0	32.0	37.0
7	35.58460177916939	36.0	35.0	37.0	35.0	37.0
8	35.66769821024756	37.0	35.0	37.0	35.0	37.0
9	37.5314967478399	39.0	37.0	39.0	35.0	39.0
10	36.73491154635438	39.0	37.0	39.0	32.0	39.0
11	36.345573790034656	38.0	35.0	39.0	32.0	39.0
12	35.54648271617103	37.0	35.0	39.0	30.0	39.0
13	35.093905742705296	37.0	34.0	39.0	29.0	39.0
14	36.07791925008728	38.0	35.0	40.0	29.0	41.0
15	36.39989648070904	38.0	35.0	40.0	30.0	41.0
16	36.612141438249104	38.0	35.0	40.0	31.0	41.0
17	36.55643017800209	38.0	35.0	40.0	31.0	41.0
18	36.46926292075239	38.0	35.0	40.0	31.0	41.0
19	36.40001386744908	38.0	35.0	40.0	31.0	41.0
20	36.25485512773259	38.0	35.0	40.0	31.0	41.0
21	36.11528411848641	38.0	34.0	40.0	30.0	41.0
22	35.98102531539323	37.0	34.0	40.0	30.0	41.0
23	35.94295369366805	37.0	34.0	40.0	30.0	41.0
24	35.80978603985797	37.0	34.0	40.0	30.0	41.0
25	35.647464507237224	37.0	34.0	40.0	30.0	41.0
26	35.40428419025653	37.0	34.0	40.0	29.0	41.0
27	35.47979184229061	37.0	34.0	40.0	29.0	41.0
28	35.54422438992347	37.0	34.0	40.0	29.0	41.0
29	35.56769322283166	37.0	34.0	40.0	29.0	41.0
30	35.51240346005019	37.0	34.0	40.0	29.0	41.0
31	35.37917072654485	37.0	34.0	40.0	29.0	41.0
32	35.21011070846851	37.0	34.0	40.0	29.0	41.0
33	35.08518263065509	36.0	34.0	40.0	27.0	41.0
34	34.93118947436286	36.0	34.0	40.0	27.0	41.0
35	34.78712431681525	36.0	33.0	40.0	26.0	41.0
36	34.66078394879262	36.0	33.0	40.0	25.0	41.0
37	34.580641649301946	36.0	33.0	40.0	25.0	41.0
38	34.536093077345086	36.0	33.0	40.0	25.0	41.0
39	34.54046010736325	36.0	33.0	40.0	25.0	41.0
40	34.27570800018003	36.0	33.0	40.0	23.0	41.0
41	34.25100995171938	36.0	33.0	40.0	23.0	41.0
42	34.186960583600644	36.0	33.0	40.0	23.0	41.0
43	34.11444781399128	36.0	33.0	40.0	23.0	41.0
44	33.9131976756209	35.0	33.0	40.0	23.0	41.0
45	33.766349296713535	35.0	33.0	40.0	23.0	41.0
46	33.730382121208805	35.0	33.0	40.0	23.0	41.0
47	33.704490134039844	35.0	33.0	40.0	23.0	41.0
48	33.65691444392137	35.0	33.0	40.0	23.0	41.0
49	33.549107191732084	35.0	32.0	39.0	23.0	41.0
50	33.408424353673475	35.0	32.0	39.0	23.0	41.0
51	33.04636411298808	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	13.0
14	24.0
15	52.0
16	163.0
17	486.0
18	1064.0
19	2029.0
20	3312.0
21	5173.0
22	7842.0
23	11358.0
24	16345.0
25	22215.0
26	27539.0
27	32133.0
28	37279.0
29	44149.0
30	52865.0
31	64292.0
32	79587.0
33	103482.0
34	167797.0
35	200306.0
36	128860.0
37	157048.0
38	215225.0
39	263460.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.659606432063484	25.44883701976355	30.311749986923235	11.579806561249725
2	29.621601106476465	28.279073897689855	29.08697445104973	13.012350544783954
3	28.917463132656746	26.89439694234912	30.572007945805034	13.616131979189095
4	25.558073592362685	29.51425002037542	29.873769720059997	15.053906667201902
5	23.045328311857034	33.79673725684827	28.87732051689092	14.280613914403778
6	21.200227718111254	42.92954727644516	25.78773801225931	10.08248699318427
7	90.78927681253035	3.8785673708654627	4.307850070979443	1.024305745624759
8	91.38983467324519	3.2069692446741085	3.918709986631293	1.4844860954494088
9	87.04506556019021	5.661142799448708	5.431052624536383	1.862739015824706
10	46.76462681356431	29.897003779488095	11.748223081030911	11.590146325916681
11	41.4588069857883	23.48799188389296	20.244650996449202	14.808550133869542
12	38.52967329992981	23.247805232894077	22.957744422913407	15.26477704426271
13	23.687062764804416	36.53963353441135	23.286974694338312	16.48632900644593
14	17.906465272379812	39.03036119839089	26.1441557825438	16.919017746685498
15	16.693610876945854	26.326074818537133	40.59239552884247	16.38791877567455
16	19.49234188371049	22.573287643737935	39.73036326634382	18.204007206207752
17	20.1037260862531	22.863287631573506	26.551116755406177	30.48186952676722
18	22.846987296686773	25.535386932240485	31.08017696811338	20.537448802959364
19	28.299935893459065	26.971884355206193	24.553230933169843	20.174948818164896
20	30.52821600133322	25.926290858796524	24.53468017891442	19.010812960955832
21	24.88921246270082	28.420302918611455	27.508153208550624	19.182331410137106
22	25.036949453148093	25.447377288281153	25.33455220912113	24.18112104944962
23	21.547157233760185	30.006605284957832	24.895598787936294	23.550638693345693
24	21.88563247124025	26.060342866596358	34.68212522306522	17.37189943909818
25	20.37037037037037	26.838258102422056	32.06470503084291	20.72666649636466
26	18.998161954775085	33.90226367859632	25.79734791118507	21.30222645544352
27	19.38389599899765	34.00706023460317	27.446479553419483	19.16256421297969
28	16.921754743214986	30.462649728915697	34.15297256069746	18.462622967171853
29	18.461649812850258	27.253004309857204	33.027276299191435	21.258069578101107
30	20.09490687521364	30.578759203911105	30.423601911761665	18.902732009113592
31	27.727052108764592	28.910286119534977	24.655472959082513	18.707188812617918
32	27.880141447980645	28.493897714182143	26.018314764332434	17.607646073504778
33	27.028448950148952	29.056867489225358	24.36097213250956	19.55371142811613
34	20.94380155437074	29.403614538438987	27.372337358542897	22.280246548647376
35	20.766444179259892	27.789455629636926	29.126143912493962	22.317956278609216
36	28.166309640674932	27.15343845832892	25.537211596593473	19.14304030440267
37	21.199558674515156	31.725986504782444	27.835011416316636	19.239443404385764
38	21.451483999518288	32.51399821669471	24.262136146722476	21.772381637064527
39	21.234409763657307	30.563979422651872	26.923591571996997	21.278019241693823
40	23.757677275265216	26.975837794637673	26.598497206438875	22.667987723658236
41	19.14760196528515	25.600527449642307	28.43423118983929	26.817639395233243
42	22.49938873744175	26.695386883582767	25.482410843858606	25.322813535116882
43	22.37093236699109	26.875298788787806	26.51699553200522	24.236773312215885
44	21.12322688241498	29.585107819416617	27.343811772491115	21.947853525677285
45	19.90611493682404	34.03181484765877	24.22643354754893	21.83563666796826
46	22.88360222803682	31.25382419237315	25.87106435104596	19.99150922854408
47	22.049913085154653	28.987773532392048	26.420409965586835	22.541903416866468
48	22.749611042382085	26.98934031084982	29.53736243551332	20.723686211254773
49	22.729296445918774	25.78615663648672	29.803155209599197	21.681391707995314
50	20.61578772584783	31.478926951387294	27.02181933633308	20.883465986431794
51	19.833614939865146	32.13708338351161	25.333822343379936	22.695479333243316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1920.0
1	2208.5
2	2497.0
3	5769.5
4	9042.0
5	7319.5
6	5597.0
7	5707.5
8	5818.0
9	6348.0
10	6878.0
11	7370.0
12	7862.0
13	7736.0
14	7610.0
15	7507.5
16	7405.0
17	7216.0
18	7027.0
19	7499.0
20	7971.0
21	8117.5
22	8264.0
23	9447.5
24	10631.0
25	10403.5
26	12133.0
27	14090.0
28	17953.0
29	21816.0
30	25142.5
31	28469.0
32	32663.5
33	36858.0
34	42392.0
35	47926.0
36	51606.5
37	55287.0
38	64563.5
39	73840.0
40	89959.0
41	106078.0
42	125017.0
43	143956.0
44	142713.0
45	141470.0
46	139814.5
47	138159.0
48	132371.0
49	126583.0
50	121530.0
51	116477.0
52	106805.5
53	97134.0
54	88038.5
55	78943.0
56	76019.5
57	73096.0
58	67888.5
59	62681.0
60	57222.5
61	51764.0
62	45662.5
63	39561.0
64	34442.5
65	29324.0
66	24472.5
67	19621.0
68	16557.0
69	13493.0
70	12173.5
71	10854.0
72	9225.5
73	7597.0
74	6098.5
75	3514.5
76	2429.0
77	1896.0
78	1363.0
79	1142.0
80	921.0
81	731.5
82	542.0
83	406.0
84	270.0
85	200.0
86	130.0
87	96.5
88	63.0
89	46.0
90	29.0
91	21.5
92	14.0
93	7.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1644138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.273120322768335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0686706447981	15.431731234213029
2	6.213692530561437	2.395144875803949
3	2.0823479691956144	1.2040002889253811
4	1.1032225829992797	0.8505016633976157
5	0.6877123171358189	0.6627181117804227
6	0.5121753863520986	0.5922730708514613
7	0.409785946009957	0.5528497690820545
8	0.34805557143279736	0.5366493525787355
9	0.2691899407097508	0.46693171052801113
>10	5.228600195217677	26.468356188200186
>50	2.4180220038803695	32.849042515253124
>100	0.6543448219354547	16.81450554910678
>500	0.0016077268352204595	0.20277181297937777
>1k	0.0025723629363527353	0.9725238572999312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4628	0.28148488752160705	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3225	0.19615141794666874	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1705	0.1037017573950605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009001677474761851	0.0
2	0.0	0.0	0.0	0.044765098793410285	0.0
3	0.0	0.0	0.0	0.0717093090725961	0.0
4	0.0	0.0	0.0	0.1491967219296677	0.0
5	0.0	0.0	0.0	0.2591631602700017	0.0
6	0.0	0.0	0.0	0.4666883193503222	0.0
7	0.0	0.0	0.0	0.5459395744152863	0.0
8	0.0	0.0	0.0	0.7432466131188501	0.0
9	0.0	0.0	0.0	0.8201258045249243	0.0
10	0.0	0.0	0.0	0.9564282316934467	0.0
11	0.0	0.0	0.0	1.180983591401695	0.0
12	0.0	0.0	0.0	1.3405200779983188	0.0
13	0.0	0.0	0.0	1.4310234299067353	0.0
14	0.0	0.0	0.0	1.4786471695198335	0.0
15	0.0	0.0	0.0	1.5354550530429927	0.0
16	0.0	0.0	0.0	1.6518078166187997	0.0
17	0.0	0.0	0.0	1.7685863352103048	0.0
18	0.0	0.0	0.0	1.913160574112392	0.0
19	0.0	0.0	0.0	1.9958786914480415	0.0
20	0.0	0.0	0.0	2.076650500140499	0.0
21	0.0	0.0	0.0	2.1969567031477895	0.0
22	0.0	0.0	0.0	2.324683207857248	0.0
23	0.0	0.0	0.0	2.471447043982926	0.0
24	0.0	0.0	0.0	2.5839071902723494	0.0
25	0.0	0.0	0.0	2.6727683442630727	0.0
26	0.0	0.0	0.0	2.7671034913127732	0.0
27	0.0	0.0	0.0	2.858458353252586	0.0
28	0.0	0.0	0.0	2.9516987016904905	0.0
29	0.0	0.0	0.0	3.061969250756323	0.0
30	0.0	0.0	0.0	3.1980283893444468	0.0
31	0.0	0.0	0.0	3.322105565347921	0.0
32	0.0	0.0	0.0	3.4214281282958	0.0
33	0.0	0.0	0.0	3.5300564794439397	0.0
34	0.0	0.0	0.0	3.6476865080668412	0.0
35	0.0	0.0	0.0	3.8024180452005854	0.0
36	0.0	0.0	0.0	3.9347670329376245	0.0
37	0.0	0.0	0.0	4.074171389506233	0.0
38	0.0	0.0	6.0822145099742234E-5	4.216069454023932	0.0
39	0.0	0.0	6.0822145099742234E-5	4.402550150899742	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGT	20	7.0341723E-4	45.000004	14
TCGATTG	40	6.8175723E-9	45.000004	1
ACTATCG	25	3.891541E-5	45.0	22
TCGTATC	25	3.891541E-5	45.0	36
TAGTACG	55	1.8189894E-12	45.0	1
ATTACGA	30	2.1660744E-6	44.999996	17
CCCCGTA	30	2.1660744E-6	44.999996	40
GCGTAAG	105	0.0	42.857143	1
TATTACG	65	0.0	41.53846	1
TACGGGA	620	0.0	39.919353	4
TAAGGGA	1830	0.0	39.71311	4
ATATGCG	80	0.0	39.375004	1
CGTTTTT	3090	0.0	39.32039	1
CGTAAGG	270	0.0	39.166668	2
CGAAAGG	265	0.0	39.056602	2
ACGTAAG	75	0.0	39.0	1
TCACGAC	295	0.0	38.898304	25
CGACAGG	145	0.0	38.793102	2
CGCAGTA	310	0.0	38.46774	37
CGGGTAT	100	0.0	38.25	6
>>END_MODULE