FastQCFastQC Report
Thu 26 May 2016
SRR1547995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547995_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2423538
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72840.3005523329941598No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA253.89209E-545.034
TCGATAG1100.040.909091
TAGGGCG8250.039.5454525
CGTTTTT53950.038.7025031
CAATCGA356.251157E-638.57142632
ACGGGAT9650.038.47155
GGGCGAT41750.038.3173687
AGGGCGA19400.037.925266
ATAGGGC10400.037.6442344
TATGGGA28750.037.5652164
AATACGG2050.037.3170742
AGGGATT38800.037.0554126
TCGTAAG1350.036.6666641
TAAGGGA36700.036.539514
CGGGATC5800.036.4655156
GCGGGAT16000.036.4218755
TCACGAC2300.036.19565225
CTCACGA2300.036.19565224
GGCGATA9100.036.09898
TAGGGAC23650.036.0570835
Produced by FastQC (version 0.11.5)