##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547995_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2423538
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.832602170875802	31.0	31.0	31.0	30.0	33.0
2	31.181788360652895	31.0	31.0	33.0	30.0	34.0
3	31.368744372896153	31.0	31.0	34.0	30.0	34.0
4	35.246342743542705	37.0	35.0	37.0	33.0	37.0
5	35.061983760931334	35.0	35.0	37.0	32.0	37.0
6	35.08774815992157	37.0	35.0	37.0	32.0	37.0
7	35.4667923506873	35.0	35.0	37.0	35.0	37.0
8	35.53841903861215	37.0	35.0	37.0	35.0	37.0
9	37.337214436084764	39.0	37.0	39.0	35.0	39.0
10	36.48168008919192	38.0	35.0	39.0	32.0	39.0
11	36.12619566930661	38.0	35.0	39.0	31.0	39.0
12	35.56225774054296	37.0	35.0	39.0	30.0	39.0
13	35.304369067041655	37.0	35.0	39.0	30.0	39.0
14	36.27606457996532	38.0	35.0	40.0	30.0	41.0
15	36.48083463102291	38.0	35.0	40.0	30.0	41.0
16	36.58246497476004	38.0	35.0	40.0	31.0	41.0
17	36.499240779389474	38.0	35.0	40.0	30.0	41.0
18	36.42512516824576	38.0	35.0	40.0	30.0	41.0
19	36.362201046569105	38.0	35.0	40.0	30.0	41.0
20	36.244706705651	38.0	34.0	40.0	30.0	41.0
21	36.10883881333819	38.0	34.0	40.0	30.0	41.0
22	35.99358458584103	38.0	34.0	40.0	30.0	41.0
23	35.916483669742334	38.0	34.0	40.0	29.0	41.0
24	35.78501471815173	38.0	34.0	40.0	29.0	41.0
25	35.592901369815536	37.0	34.0	40.0	29.0	41.0
26	35.352034504926266	37.0	34.0	40.0	27.0	41.0
27	35.43399154459307	37.0	34.0	40.0	27.0	41.0
28	35.45638195068532	37.0	34.0	40.0	27.0	41.0
29	35.432416161826225	37.0	34.0	40.0	28.0	41.0
30	35.34635891824267	37.0	34.0	40.0	27.0	41.0
31	35.25397414853821	37.0	34.0	40.0	27.0	41.0
32	35.13127295713952	37.0	34.0	40.0	27.0	41.0
33	34.99311213605893	37.0	33.0	40.0	26.0	41.0
34	34.79283345258048	37.0	33.0	40.0	25.0	41.0
35	34.684112648532846	37.0	33.0	40.0	24.0	41.0
36	34.607563405236476	37.0	33.0	40.0	24.0	41.0
37	34.55841253572257	37.0	33.0	40.0	24.0	41.0
38	34.481423852235864	37.0	33.0	40.0	24.0	41.0
39	34.445510654258364	37.0	33.0	40.0	24.0	41.0
40	34.24080992334348	37.0	33.0	40.0	23.0	41.0
41	34.15367821754806	37.0	33.0	40.0	23.0	41.0
42	34.110266890801796	36.0	33.0	40.0	23.0	41.0
43	34.054885048222886	36.0	33.0	40.0	23.0	41.0
44	33.89567731143477	36.0	33.0	40.0	23.0	41.0
45	33.75740755870137	36.0	32.0	40.0	23.0	41.0
46	33.69176468452321	36.0	32.0	40.0	23.0	41.0
47	33.62732500996477	35.0	32.0	40.0	23.0	41.0
48	33.5277123775241	35.0	32.0	40.0	23.0	41.0
49	33.38801784828627	35.0	32.0	39.0	22.0	41.0
50	33.2674362852986	35.0	32.0	39.0	22.0	41.0
51	32.93127155423187	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	7.0
13	19.0
14	61.0
15	152.0
16	358.0
17	929.0
18	1973.0
19	3611.0
20	5927.0
21	9036.0
22	13089.0
23	18621.0
24	25813.0
25	34542.0
26	43718.0
27	51440.0
28	60051.0
29	70336.0
30	83491.0
31	98983.0
32	119648.0
33	148993.0
34	214271.0
35	238427.0
36	210529.0
37	255526.0
38	334472.0
39	379462.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.93596469294065	24.984423598887247	27.9070515915162	13.172560116655898
2	30.468926008174822	27.247148590201597	28.59505400781832	13.688871393805254
3	30.06253667159335	26.706368953158567	29.536033682987433	13.695060692260656
4	26.518090494145337	29.147180692029583	29.159270455012464	15.175458358812612
5	23.788651137304225	33.20521485530658	28.458394297923117	14.547739709466079
6	22.062950941969962	42.03556948560328	25.513484830854726	10.387994741572031
7	90.72512995463656	3.308468858338512	4.682245543498802	1.2841556435261177
8	91.03480118735501	2.8996450643645777	4.411525629059664	1.6540281192207424
9	85.73346075035754	5.868940367347242	6.031512606775713	2.366086275519509
10	40.84644020436238	36.303907757996775	11.543743073143478	11.305908964497359
11	36.1722407488556	25.03385546255103	22.848703011877678	15.945200776715692
12	33.19506440583972	22.890583931425873	25.705064249044167	18.209287413690234
13	25.759199979534053	28.5776414481638	26.879545523940617	18.783613048361527
14	20.76798465714175	31.140506152575288	28.866434114092705	19.225075076190265
15	20.714963000373835	25.268925017887074	35.37848385294557	18.637628128793523
16	24.471784638821426	24.121222774307643	32.7490635591437	18.65792902772723
17	24.203994325651177	23.88194449602193	27.270214042445385	24.643847135881508
18	25.39729106785204	24.870334197359398	29.34400038291126	20.388374351877296
19	28.03285939811961	26.315741696643503	25.09768776062104	20.553711144615846
20	30.120427243146175	26.405527786236487	24.83356976453433	18.640475206083007
21	27.197056534702575	26.408209815567158	27.59044834452771	18.80428530520256
22	26.3731371243199	23.390514198663276	27.650443277555375	22.58590539946145
23	23.9280341385198	26.998297530304864	26.84773252987987	22.225935801295464
24	23.590346014793248	25.753918444852115	30.492321556336233	20.163413984018405
25	23.552550032225614	26.21031731295321	28.654553796969555	21.58257885785162
26	22.053997090204486	29.867037364382153	26.286404421964914	21.792561123448444
27	21.723447290696495	28.687068244855247	27.938534489659332	21.650949974788926
28	20.84225623860653	27.93160247538929	30.76147351516667	20.46466777083751
29	22.16243359914307	26.01180588049373	30.405217496073924	21.42054302428928
30	23.094170588618788	26.689905419267202	29.1597655988889	21.05615839322511
31	26.570121863160388	26.689410275390774	26.36884587739082	20.371621984058017
32	27.671651940262542	26.257933649070083	26.05298534621698	20.017429064450404
33	26.88045328771408	26.48941341130199	25.631948003291054	20.998185297692878
34	22.721946179511114	27.617598733752057	27.88897884002644	21.77147624671039
35	22.645363926622977	27.624737057970624	27.828364977153235	21.901534038253164
36	26.572886416470464	27.43299259182237	25.567455513385802	20.42666547832136
37	23.192250338142006	29.322007742399748	26.089295897155317	21.396446022302932
38	23.146160695644134	30.614457045856096	24.576631354655877	21.662750903843882
39	23.093675444742356	28.41383134904425	25.632030527270462	22.860462678942934
40	24.70706050410598	26.052077582443516	26.453102860363646	22.78775905308685
41	21.220174802293176	25.65608626726711	27.30326489619721	25.8204740342425
42	23.12986220971159	26.451163546847624	25.963240518613695	24.45573372482709
43	22.371384314997332	25.732998616072866	27.285893598532397	24.60972347039741
44	22.056018927699917	26.88032950174497	27.49888798937751	23.5647635811776
45	21.8166581254348	29.02954275938731	26.03549026258305	23.118308852594843
46	23.131017545423262	28.15437595779394	26.340952772351827	22.373653724430977
47	22.114033285221854	27.28040575390194	27.36313604325577	23.24242491762044
48	22.837768584606472	25.939803708462588	28.870312741124753	22.352114965806187
49	23.35420364772494	25.939143516627343	28.156480319268773	22.550172516378947
50	21.6738916410636	28.31727829314003	27.25568982207005	22.75314024372632
51	21.140786734105262	28.977387604403148	26.720563077616276	23.16126258387531
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1930.0
1	2414.5
2	2899.0
3	8673.5
4	14448.0
5	11347.5
6	8247.0
7	8064.0
8	7881.0
9	8379.0
10	8877.0
11	9063.0
12	9249.0
13	9228.5
14	9208.0
15	9314.0
16	9420.0
17	9553.0
18	9686.0
19	9692.5
20	9699.0
21	10100.5
22	10502.0
23	11385.0
24	12268.0
25	14420.5
26	19891.0
27	23209.0
28	27314.5
29	31420.0
30	36344.0
31	41268.0
32	45182.5
33	49097.0
34	57756.0
35	66415.0
36	72042.0
37	77669.0
38	87412.0
39	97155.0
40	110812.5
41	124470.0
42	144802.5
43	165135.0
44	169483.0
45	173831.0
46	174761.0
47	175691.0
48	177567.0
49	179443.0
50	173342.5
51	167242.0
52	157772.0
53	148302.0
54	141120.0
55	133938.0
56	129095.0
57	124252.0
58	120952.0
59	117652.0
60	110416.0
61	103180.0
62	93415.5
63	83651.0
64	75210.5
65	66770.0
66	56488.5
67	46207.0
68	39646.5
69	33086.0
70	28163.0
71	23240.0
72	19801.5
73	16363.0
74	13461.0
75	8396.0
76	6233.0
77	4817.0
78	3401.0
79	2664.0
80	1927.0
81	1442.0
82	957.0
83	708.0
84	459.0
85	342.0
86	225.0
87	169.0
88	113.0
89	81.5
90	50.0
91	36.5
92	23.0
93	17.0
94	11.0
95	8.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2423538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.9929999610289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51269334944399	16.272239684641228
2	6.161397224941539	2.5869242340616263
3	2.3425293559262954	1.4753015603300936
4	1.2890921050582893	1.0824764204500534
5	0.8762359835219397	0.91974109839641
6	0.672748035836169	0.847379969405458
7	0.5607790516034186	0.8240704225919474
8	0.4778556500094881	0.80252989136213
9	0.4262236456368116	0.8052941678618866
>10	7.4553264072590855	39.67884661661342
>50	2.044890540352363	28.866343332547988
>100	0.17660397276978856	4.924343275145021
>500	0.0022150807805033073	0.3003131445530054
>1k	0.0012082258802745313	0.3062737517295219
>5k	2.0137098004575522E-4	0.30792243031026056
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7284	0.3005523329941598	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017742655572142874	0.0
2	0.0	0.0	0.0	0.05685902181026252	0.0
3	0.0	0.0	0.0	0.08920842173714627	0.0
4	0.0	0.0	0.0	0.19397261359219456	0.0
5	0.0	0.0	0.0	0.34362985024373455	0.0
6	0.0	0.0	0.0	0.6903543497151685	0.0
7	0.0	0.0	0.0	0.8273028935382899	0.0
8	4.126198970265785E-5	0.0	0.0	1.1632992756870328	0.0
9	4.126198970265785E-5	0.0	0.0	1.3044152804701226	0.0
10	4.126198970265785E-5	0.0	0.0	1.5225674200280745	0.0
11	4.126198970265785E-5	0.0	0.0	1.7655592773870268	0.0
12	4.126198970265785E-5	0.0	0.0	1.9459154343773442	0.0
13	4.126198970265785E-5	0.0	0.0	2.055342231068793	0.0
14	4.126198970265785E-5	0.0	0.0	2.116946381694861	0.0
15	4.126198970265785E-5	0.0	0.0	2.17685879074312	0.0
16	4.126198970265785E-5	0.0	0.0	2.272669130832692	0.0
17	4.126198970265785E-5	0.0	0.0	2.3849430048136235	0.0
18	0.0030946492276993386	0.0	0.0	2.514216818552051	0.0
19	0.0030946492276993386	0.0	0.0	2.606561151506599	0.0
20	0.0030946492276993386	0.0	0.0	2.705713712762086	0.0
21	0.0030946492276993386	0.0	0.0	2.8239293132602006	0.0
22	0.0030946492276993386	0.0	0.0	2.956999230051272	0.0
23	0.0030946492276993386	0.0	0.0	3.1051710350735164	0.0
24	0.0030946492276993386	0.0	0.0	3.224748281231819	0.0
25	0.0030946492276993386	0.0	0.0	3.333803720015944	0.0
26	0.0030946492276993386	0.0	0.0	3.4436431366044187	0.0
27	0.0030946492276993386	0.0	0.0	3.5559995345647564	0.0
28	0.0030946492276993386	0.0	0.0	3.6737612531761417	0.0
29	0.0030946492276993386	0.0	0.0	3.8007656574809223	0.0
30	0.0030946492276993386	0.0	0.0	3.961934989259504	0.0
31	0.0030946492276993386	0.0	0.0	4.110808248106694	0.0
32	0.0030946492276993386	0.0	0.0	4.247344172032788	0.0
33	0.0030946492276993386	0.0	0.0	4.394113069405142	0.0
34	0.0030946492276993386	0.0	0.0	4.539190225199687	0.0
35	0.0030946492276993386	0.0	0.0	4.707621667165936	0.0
36	0.0030946492276993386	0.0	0.0	4.866108969613846	0.0
37	0.0030946492276993386	0.0	0.0	5.027113253433616	0.0
38	0.0030946492276993386	0.0	0.0	5.225335851965185	0.0
39	0.0030946492276993386	0.0	0.0	5.511817846470739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	25	3.89209E-5	45.0	34
TCGATAG	110	0.0	40.90909	1
TAGGGCG	825	0.0	39.545452	5
CGTTTTT	5395	0.0	38.702503	1
CAATCGA	35	6.251157E-6	38.571426	32
ACGGGAT	965	0.0	38.4715	5
GGGCGAT	4175	0.0	38.317368	7
AGGGCGA	1940	0.0	37.92526	6
ATAGGGC	1040	0.0	37.644234	4
TATGGGA	2875	0.0	37.565216	4
AATACGG	205	0.0	37.317074	2
AGGGATT	3880	0.0	37.055412	6
TCGTAAG	135	0.0	36.666664	1
TAAGGGA	3670	0.0	36.53951	4
CGGGATC	580	0.0	36.465515	6
GCGGGAT	1600	0.0	36.421875	5
TCACGAC	230	0.0	36.195652	25
CTCACGA	230	0.0	36.195652	24
GGCGATA	910	0.0	36.0989	8
TAGGGAC	2365	0.0	36.057083	5
>>END_MODULE