FastQCFastQC Report
Thu 26 May 2016
SRR1547994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547994_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3674814
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.17881177115358762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT3650.041.3013728
CGGTCTA3650.041.3013731
CCGTCGA556.184564E-1140.90909219
GACCGAT20300.039.7906389
AACACGT28850.039.77469641
GACACGA30000.039.67525
CACGACC30250.039.4958727
TAACGGG8050.039.409943
GGGCGAT68050.039.1476867
GGCGATT18500.039.040548
GCGAGAC30450.039.0147821
AGGGCGA35300.038.881026
ACGACCA30600.038.8235328
GCGATAC2500.038.6999979
ACACGAC30700.038.69706726
GGACCGA22200.038.513518
CACGTGA29900.038.37792643
CGAGACA31050.038.26086822
AAACACG30700.038.25732840
TCAAGCG31150.037.99357617
Produced by FastQC (version 0.11.5)