##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547994_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3674814
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.929700931802262	31.0	31.0	33.0	30.0	33.0
2	31.293760446106933	31.0	31.0	33.0	30.0	34.0
3	31.506470259447145	31.0	31.0	34.0	30.0	34.0
4	35.36858600190377	37.0	35.0	37.0	33.0	37.0
5	35.194803600944155	37.0	35.0	37.0	32.0	37.0
6	35.22760988719429	37.0	35.0	37.0	32.0	37.0
7	35.54673950844859	35.0	35.0	37.0	35.0	37.0
8	35.61736730076679	37.0	35.0	37.0	35.0	37.0
9	37.43819333441094	39.0	37.0	39.0	35.0	39.0
10	36.602993239929965	39.0	35.0	39.0	32.0	39.0
11	36.2626481231431	38.0	35.0	39.0	31.0	39.0
12	35.80795898785625	37.0	35.0	39.0	31.0	39.0
13	35.63941331452422	37.0	35.0	39.0	30.0	39.0
14	36.64597228594427	38.0	35.0	40.0	31.0	41.0
15	36.80056351151378	38.0	35.0	40.0	31.0	41.0
16	36.89831784683524	38.0	35.0	40.0	31.0	41.0
17	36.800894956860404	38.0	35.0	40.0	31.0	41.0
18	36.737221258001085	38.0	35.0	40.0	31.0	41.0
19	36.7088252629929	38.0	35.0	40.0	31.0	41.0
20	36.62177214955641	38.0	35.0	40.0	30.0	41.0
21	36.50431341559056	38.0	35.0	40.0	30.0	41.0
22	36.385569718630656	38.0	35.0	40.0	30.0	41.0
23	36.27112610325312	38.0	34.0	40.0	30.0	41.0
24	36.172169802335574	38.0	34.0	40.0	30.0	41.0
25	36.004207287770214	38.0	34.0	40.0	30.0	41.0
26	35.74880007532354	38.0	34.0	40.0	29.0	41.0
27	35.864466337616	38.0	34.0	40.0	29.0	41.0
28	35.881909397319156	38.0	34.0	40.0	29.0	41.0
29	35.87204495247923	38.0	34.0	40.0	29.0	41.0
30	35.789147695638476	38.0	34.0	40.0	29.0	41.0
31	35.71412158547344	38.0	34.0	40.0	29.0	41.0
32	35.60212326392574	38.0	34.0	40.0	28.0	41.0
33	35.52092758980454	38.0	34.0	40.0	27.0	41.0
34	35.39298179445272	38.0	34.0	40.0	27.0	41.0
35	35.285292262411105	38.0	34.0	40.0	27.0	41.0
36	35.239432526380924	38.0	34.0	40.0	26.0	41.0
37	35.197558570311315	38.0	34.0	40.0	26.0	41.0
38	35.10632837471502	38.0	34.0	40.0	26.0	41.0
39	35.081995170367804	38.0	34.0	40.0	26.0	41.0
40	34.891562130763624	37.0	33.0	40.0	25.0	41.0
41	34.82792108661826	37.0	33.0	40.0	25.0	41.0
42	34.797031087831925	37.0	33.0	40.0	25.0	41.0
43	34.752096840819696	37.0	33.0	40.0	25.0	41.0
44	34.611290530622774	37.0	33.0	40.0	24.0	41.0
45	34.44954084750956	37.0	33.0	40.0	24.0	41.0
46	34.38365234267639	37.0	33.0	40.0	24.0	41.0
47	34.3245875845689	36.0	33.0	40.0	24.0	41.0
48	34.23037601358872	36.0	33.0	40.0	23.0	41.0
49	34.095576810146035	36.0	33.0	40.0	24.0	41.0
50	33.993253536097335	36.0	33.0	40.0	24.0	41.0
51	33.677370337655184	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	4.0
12	11.0
13	21.0
14	83.0
15	184.0
16	455.0
17	1054.0
18	2130.0
19	4106.0
20	6958.0
21	10808.0
22	15852.0
23	22437.0
24	31367.0
25	42695.0
26	53698.0
27	65474.0
28	77644.0
29	92744.0
30	113095.0
31	137990.0
32	168409.0
33	212930.0
34	308601.0
35	351710.0
36	325202.0
37	404286.0
38	556330.0
39	668464.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.09350405217788	24.92409139619039	28.38372227818877	11.598682273442956
2	31.515554256623602	26.233354939869063	28.1265391935483	14.124551609959035
3	30.612406505472112	26.3907506611219	29.133284024715266	13.863558808690726
4	26.928655436710535	29.706591952681144	27.47785330087455	15.88689930973377
5	23.843002666257394	33.07487127239637	27.428680744113855	15.65344531723238
6	22.47240268487058	42.52786671651953	24.691943592247117	10.307787006362771
7	91.71155329222105	3.1118853906619495	3.873937565275413	1.3026237518415897
8	92.03410022929052	2.689986486390876	3.9211508391989365	1.3547624451196714
9	86.94176086191028	5.58382002463254	5.270334770684992	2.204084342772178
10	39.21186215139052	38.115289644591535	11.417067639341747	11.255780564676199
11	33.37733556038482	26.85235225510733	23.30605031982571	16.464261864682133
12	30.531722149746898	24.301556486940566	26.584093779984514	18.582627583328026
13	25.023007967205956	28.95098908407337	27.430259055288243	18.595743893432427
14	21.106809759623207	31.42676064693342	28.429602151292556	19.036827442150813
15	20.767608918437777	27.422340287154668	33.872489872956834	17.937560921450718
16	24.3050668686905	25.307811497398237	32.869445909371194	17.517675724540073
17	23.119102082445533	25.547850857213454	27.429850871363833	23.903196188977184
18	24.90732864302792	25.880874515009467	29.008134833490896	20.20366200847172
19	26.688888199511595	28.0346433860326	25.84925386699844	19.427214547457368
20	29.45923249448816	27.403291704015498	25.001238157904048	18.136237643592303
21	27.27297218308192	26.994046501401158	27.25019552009979	18.48278579541713
22	26.0764218270639	24.34207554450375	26.943785454175366	22.637717174256984
23	23.587425105052937	29.04933419759476	26.929281318727966	20.433959378624333
24	22.902002659182205	27.325382998976274	30.248469718467387	19.524144623374134
25	23.679837945539557	27.69699364375993	27.79229098397905	20.83087742672146
26	22.25598356814794	31.093274380689746	25.43946441915155	21.211277632010763
27	22.010175208867715	29.45229336777317	27.755255095904175	20.78227632745494
28	20.065940752375493	30.035479346709792	29.98410259675728	19.914477304157437
29	21.484053342563733	28.144880257885163	29.52312688478927	20.847939514761833
30	22.882192132717467	27.12744100789863	29.638098690165	20.352268169218902
31	25.763208695732626	27.464845839816654	26.330856473280008	20.44108899117071
32	25.331159617874537	27.634623140109948	26.808649362933746	20.225567879081773
33	25.414184228099707	27.58150480541328	25.494215489545862	21.510095476941146
34	21.747168700238976	29.119623469378315	28.223714179819716	20.909493650562993
35	21.760339434866633	28.396348767583884	28.667736652793856	21.175575144755626
36	25.041675578682348	29.468103691778687	25.523822430196468	19.966398299342497
37	22.723762345522793	30.059235651110505	26.503300575212783	20.713701428153914
38	22.59202778698459	30.542851964752504	25.919162167119207	20.945958081143697
39	22.972618478105286	28.40785955425227	25.880956151794347	22.738565815848094
40	24.41726302338023	26.560936145339603	27.392842195550575	21.62895863572959
41	20.4751587427282	27.504711803100783	27.853137600977902	24.166991853193114
42	22.92170433660044	27.947700210133085	26.028990855047358	23.101604598219122
43	22.571482529455913	27.427537829125505	26.507600112549913	23.493379528868672
44	22.165067402050827	28.21865269915702	28.124307788094853	21.4919721106973
45	22.010229633390967	29.11570490370397	25.767236110453485	23.10682935245158
46	22.71516327084854	28.83073809994193	26.44819574541732	22.005902883792213
47	22.175816245393644	28.39379081499091	27.97474919819071	21.455643741424733
48	23.012865413052197	27.303613189674365	28.556030318813413	21.127491078460025
49	22.78773837260879	27.270387018227314	28.302330403661248	21.639544205502645
50	21.28883257764883	29.59638229309021	27.675060560888255	21.43972456837271
51	21.550696171289214	28.27590729762105	27.331451333319183	22.841945197770556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1353.0
1	2665.5
2	3978.0
3	9528.0
4	15078.0
5	12154.0
6	9230.0
7	9273.5
8	9317.0
9	9814.0
10	10311.0
11	10702.5
12	11094.0
13	11669.5
14	12245.0
15	12310.0
16	12375.0
17	12470.5
18	12566.0
19	13090.5
20	13615.0
21	14922.5
22	16230.0
23	19544.5
24	22859.0
25	27080.0
26	36134.0
27	40967.0
28	50631.5
29	60296.0
30	69908.5
31	79521.0
32	91025.5
33	102530.0
34	110326.5
35	118123.0
36	128328.0
37	138533.0
38	155834.5
39	173136.0
40	192810.5
41	212485.0
42	230827.0
43	249169.0
44	251083.0
45	252997.0
46	257850.5
47	262704.0
48	265931.5
49	269159.0
50	265810.0
51	262461.0
52	249285.5
53	236110.0
54	221383.5
55	206657.0
56	191695.5
57	176734.0
58	166924.0
59	157114.0
60	148666.0
61	140218.0
62	123910.0
63	107602.0
64	92854.5
65	78107.0
66	66309.0
67	54511.0
68	45197.5
69	35884.0
70	30506.5
71	25129.0
72	22308.0
73	19487.0
74	17430.0
75	12456.0
76	9539.0
77	6947.0
78	4355.0
79	3256.0
80	2157.0
81	1677.0
82	1197.0
83	871.0
84	545.0
85	390.5
86	236.0
87	167.0
88	98.0
89	76.5
90	55.0
91	45.0
92	35.0
93	27.0
94	19.0
95	17.5
96	16.0
97	9.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3674814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.77058854003871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33315033255681	13.304636218123452
2	6.749354487161704	2.2638129403010585
3	2.2112708661493787	1.112529415402987
4	1.176384619859239	0.7891464969795657
5	0.7352272877393466	0.6165097163042614
6	0.5638421875310612	0.5673579197159263
7	0.43140449170710904	0.5064435057321126
8	0.34461444693992577	0.46235096756659966
9	0.2885767084517822	0.4355641115715195
>10	4.637512093109579	19.816915345728432
>50	2.137218875438687	25.86808770352048
>100	1.3837738825165564	32.92506174537468
>500	0.005835657160042102	0.606022934444147
>1k	0.0016673306171548863	0.5418219167039282
>5k	1.6673306171548864E-4	0.18373906253091357
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6571	0.17881177115358762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.721226162739121E-5	0.0	0.0	0.009823626447488227	0.0
2	2.721226162739121E-5	0.0	0.0	0.040355783993421164	0.0
3	2.721226162739121E-5	0.0	0.0	0.06335014506856673	0.0
4	2.721226162739121E-5	0.0	0.0	0.10844086258515397	0.0
5	2.721226162739121E-5	0.0	0.0	0.19571058562419757	0.0
6	2.721226162739121E-5	0.0	0.0	0.3562901414874331	0.0
7	2.721226162739121E-5	0.0	0.0	0.4257358331605355	0.0
8	2.721226162739121E-5	0.0	0.0	0.5943974307271062	0.0
9	2.721226162739121E-5	0.0	0.0	0.6781295597545889	0.0
10	2.721226162739121E-5	0.0	0.0	0.8247220131413454	0.0
11	2.721226162739121E-5	0.0	0.0	1.0155071794109851	0.0
12	2.721226162739121E-5	0.0	0.0	1.1633241845709743	0.0
13	2.721226162739121E-5	0.0	0.0	1.2440085402961891	0.0
14	2.721226162739121E-5	0.0	0.0	1.2860242722488813	0.0
15	2.721226162739121E-5	0.0	0.0	1.3368295647072206	0.0
16	2.721226162739121E-5	0.0	0.0	1.4281811269903728	0.0
17	2.721226162739121E-5	0.0	0.0	1.535043678401138	0.0
18	2.721226162739121E-5	0.0	0.0	1.6587506197592585	0.0
19	2.721226162739121E-5	0.0	0.0	1.7349993768392087	0.0
20	2.721226162739121E-5	0.0	0.0	1.8076016908610886	0.0
21	2.721226162739121E-5	0.0	0.0	1.9033888517895055	0.0
22	2.721226162739121E-5	0.0	0.0	2.013925058519969	0.0
23	2.721226162739121E-5	0.0	0.0	2.136244174535092	0.0
24	2.721226162739121E-5	0.0	0.0	2.2287114395449676	0.0
25	2.721226162739121E-5	0.0	0.0	2.312171445956176	0.0
26	2.721226162739121E-5	0.0	0.0	2.4002303245824144	0.0
27	2.721226162739121E-5	0.0	0.0	2.490575033185353	0.0
28	2.721226162739121E-5	0.0	0.0	2.5881037788579233	0.0
29	2.721226162739121E-5	0.0	0.0	2.687700656414175	0.0
30	2.721226162739121E-5	0.0	0.0	2.811434810033923	0.0
31	2.721226162739121E-5	0.0	0.0	2.9255630352992017	0.0
32	2.721226162739121E-5	0.0	0.0	3.040997449122595	0.0
33	2.721226162739121E-5	0.0	0.0	3.1605409144517247	0.0
34	2.721226162739121E-5	0.0	0.0	3.272437734263557	0.0
35	2.721226162739121E-5	0.0	0.0	3.4050430851738347	0.0
36	2.721226162739121E-5	0.0	0.0	3.5254573428750406	0.0
37	2.721226162739121E-5	0.0	0.0	3.6604573728085286	0.0
38	2.721226162739121E-5	0.0	0.0	3.8061790338232084	0.0
39	2.721226162739121E-5	0.0	0.0	4.006815038801964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	365	0.0	41.30137	28
CGGTCTA	365	0.0	41.30137	31
CCGTCGA	55	6.184564E-11	40.909092	19
GACCGAT	2030	0.0	39.790638	9
AACACGT	2885	0.0	39.774696	41
GACACGA	3000	0.0	39.675	25
CACGACC	3025	0.0	39.49587	27
TAACGGG	805	0.0	39.40994	3
GGGCGAT	6805	0.0	39.147686	7
GGCGATT	1850	0.0	39.04054	8
GCGAGAC	3045	0.0	39.01478	21
AGGGCGA	3530	0.0	38.88102	6
ACGACCA	3060	0.0	38.82353	28
GCGATAC	250	0.0	38.699997	9
ACACGAC	3070	0.0	38.697067	26
GGACCGA	2220	0.0	38.51351	8
CACGTGA	2990	0.0	38.377926	43
CGAGACA	3105	0.0	38.260868	22
AAACACG	3070	0.0	38.257328	40
TCAAGCG	3115	0.0	37.993576	17
>>END_MODULE