FastQCFastQC Report
Thu 26 May 2016
SRR1547993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547993_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1996039
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82730.41447085953731366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG501.0822987E-940.5000041
CGGTCTA900.040.031
GGGCGAT34000.038.9779437
CGTTTTT57600.038.6328121
CACGACC10250.038.41463527
ACGGGAT6500.038.076925
AGGGCGA18100.038.0386776
CGTTAGG2900.038.017242
TACGGGA7900.037.879754
TAATACG601.5643309E-1037.5000041
TAAGGGA26450.037.3440444
GTAGGGA28650.037.2251324
GGCGATA8650.037.1965338
ACACGAC10550.037.10900526
TCGTTAG850.037.0588231
TTACGCG850.037.0588231
ACGACCA10750.036.8372128
GCGATCG1100.036.818189
TAGGGAC20650.036.7191285
TAGGGAG29450.036.6723255
Produced by FastQC (version 0.11.5)