##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547993_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1996039
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89857162109558	31.0	31.0	31.0	30.0	33.0
2	31.249159460311148	31.0	31.0	33.0	30.0	34.0
3	31.405190479745134	31.0	31.0	34.0	30.0	34.0
4	35.313123140379524	37.0	35.0	37.0	33.0	37.0
5	35.162066973641295	37.0	35.0	37.0	32.0	37.0
6	35.20219144014721	37.0	35.0	37.0	32.0	37.0
7	35.5186551966169	35.0	35.0	37.0	35.0	37.0
8	35.58288189759819	37.0	35.0	37.0	35.0	37.0
9	37.38945030633169	39.0	37.0	39.0	35.0	39.0
10	36.569770430337286	38.0	35.0	39.0	32.0	39.0
11	36.25014290802935	38.0	35.0	39.0	31.0	39.0
12	35.78405832751765	37.0	35.0	39.0	30.0	39.0
13	35.589822142753725	37.0	35.0	39.0	30.0	39.0
14	36.569466829054946	38.0	35.0	40.0	30.0	41.0
15	36.73067109410187	38.0	35.0	40.0	31.0	41.0
16	36.813128901789995	38.0	35.0	40.0	31.0	41.0
17	36.729066416036964	38.0	35.0	40.0	31.0	41.0
18	36.62605189577959	38.0	35.0	40.0	31.0	41.0
19	36.572603040321354	38.0	35.0	40.0	30.0	41.0
20	36.46383161852048	38.0	35.0	40.0	30.0	41.0
21	36.346316880581995	38.0	35.0	40.0	30.0	41.0
22	36.21662302189486	38.0	34.0	40.0	30.0	41.0
23	36.12737175977023	38.0	34.0	40.0	30.0	41.0
24	35.99864832300371	38.0	34.0	40.0	30.0	41.0
25	35.81703714205985	38.0	34.0	40.0	29.0	41.0
26	35.59826085562457	38.0	34.0	40.0	29.0	41.0
27	35.70487550593951	38.0	34.0	40.0	29.0	41.0
28	35.723086071965525	38.0	34.0	40.0	29.0	41.0
29	35.6896979467836	38.0	34.0	40.0	29.0	41.0
30	35.593562550631525	38.0	34.0	40.0	29.0	41.0
31	35.48804306929875	38.0	34.0	40.0	28.0	41.0
32	35.357011060405135	38.0	34.0	40.0	27.0	41.0
33	35.20103865706031	38.0	34.0	40.0	27.0	41.0
34	35.01279684414984	38.0	34.0	40.0	25.0	41.0
35	34.84642734936542	38.0	33.0	40.0	25.0	41.0
36	34.78120116891504	37.0	33.0	40.0	24.0	41.0
37	34.73252827224318	37.0	33.0	40.0	24.0	41.0
38	34.63666240990281	37.0	33.0	40.0	24.0	41.0
39	34.58912676555919	37.0	33.0	40.0	24.0	41.0
40	34.38824241410113	37.0	33.0	40.0	23.0	41.0
41	34.29224378882377	37.0	33.0	40.0	23.0	41.0
42	34.24677123042185	37.0	33.0	40.0	23.0	41.0
43	34.194712127368255	37.0	33.0	40.0	23.0	41.0
44	34.041845875756934	36.0	33.0	40.0	23.0	41.0
45	33.90889807263285	36.0	33.0	40.0	23.0	41.0
46	33.82634106848614	36.0	33.0	40.0	23.0	41.0
47	33.75627079430813	36.0	32.0	40.0	23.0	41.0
48	33.66015142990693	36.0	32.0	40.0	23.0	41.0
49	33.510430908414115	36.0	32.0	40.0	22.0	41.0
50	33.40834622970794	35.0	32.0	39.0	22.0	41.0
51	33.09362943309224	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	4.0
13	12.0
14	35.0
15	111.0
16	285.0
17	633.0
18	1397.0
19	2559.0
20	4228.0
21	6779.0
22	9799.0
23	13793.0
24	19801.0
25	27362.0
26	34951.0
27	41764.0
28	47191.0
29	54433.0
30	64369.0
31	77912.0
32	94622.0
33	118583.0
34	170355.0
35	188598.0
36	177331.0
37	219429.0
38	289430.0
39	330236.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.17913076848699	25.510222996644856	28.339376134434247	13.97127010043391
2	32.210843575701674	26.54822876707319	28.780098986041857	12.460828671183279
3	29.956779401604877	26.80353439987896	29.89585874825091	13.34382745026525
4	26.660952015466634	29.05093537751517	29.27042006694258	15.01769254007562
5	24.258143252711996	32.86624159147191	28.142987186122113	14.73262796969398
6	22.736329300179005	41.95178551120494	25.2287154709903	10.083169717625758
7	90.39587903843562	3.0274458565188356	5.335617189844487	1.2410579152010557
8	90.38265284395746	2.8358664334714905	5.323693575125536	1.4577871474455157
9	84.98285855136098	5.745829615553604	6.802472296382986	2.4688395367024394
10	39.2726795418326	36.65810136976281	13.244380495571479	10.824838592833105
11	33.9144175038664	25.907509823204855	23.54192478203081	16.636147890897924
12	31.495126097235577	22.556272698078544	27.370607488130243	18.57799371655564
13	25.870336200845774	27.258735926502435	27.92786112896592	18.94306674368587
14	21.587954944768114	28.802543437277528	29.58609526166573	20.02340635628863
15	21.353741084217294	25.685169478151483	34.28590323134969	18.67518620628154
16	25.44945264095541	24.979822538537576	31.434005046995573	18.13671977351144
17	25.287732353926952	24.209847603178094	27.514141757751226	22.988278285143725
18	25.912619943798692	24.98277839260656	29.38439579587373	19.72020586772102
19	27.30031828035424	26.218776286435286	26.590262013918565	19.890643419291905
20	29.24737442504881	26.907540383729977	25.795187368583477	18.049897822637735
21	27.937029286501918	25.544290467270432	28.043289735320805	18.47539051090685
22	26.71711324277732	23.277250594803007	28.018390422231228	21.987245740188442
23	24.564900786006685	26.49076496000329	27.82871476960119	21.11561948438883
24	24.125079720386225	25.665229987991218	30.454815762617866	19.75487452900469
25	24.06020122853311	26.53570396169614	28.24308543069549	21.161009379075256
26	22.533577750735333	28.978491903214316	26.641964410515023	21.84596593553533
27	21.732090405047195	27.859876485379292	28.827342551924083	21.580690557649422
28	20.542835084885617	28.176102771539036	29.892251604302324	21.38881053927303
29	22.044759646479854	26.928231362212863	29.850619151229008	21.176389840078276
30	23.758854411161305	26.603388009953715	29.087658106880674	20.550099472004305
31	25.6859209664741	26.738706007247355	27.15052160804473	20.42485141823381
32	26.4969772634703	26.510554152499022	26.8068409484985	20.18562763553217
33	25.851448794337184	26.742012555866896	26.218525790327746	21.188012859468177
34	22.372057860592903	27.86689037639044	28.596335041549793	21.164716721466863
35	22.65421667612707	28.420586972499034	28.340328019642904	20.584868331730995
36	25.49684650450217	28.679750245360935	25.824846107716333	19.998557142420566
37	23.165930124611794	29.652126035613534	26.18616169323345	20.995782146541224
38	23.443780407096256	29.430537178882776	25.62685398431594	21.49882842970503
39	23.894923896777566	27.9472996269111	25.88220971634322	22.275566759968115
40	24.83403380394872	27.27982769875739	26.104399763732072	21.78173873356182
41	21.591812584824243	26.550633529705582	27.44545572506349	24.412098160406686
42	22.790787153958412	26.932890589813123	26.264416677229253	24.011905578999208
43	22.63562986494753	26.475184102114234	27.1307825147705	23.758403518167732
44	22.633976590637758	27.44380245075372	27.29230240491293	22.629918553695592
45	22.08408753536379	28.190230752004346	26.199588284597645	23.526093428034223
46	23.020742580681038	27.90441469330008	26.59832798858138	22.476514737437494
47	22.138294893035656	27.76604064349444	28.313074043142443	21.78259042032746
48	23.283412799048516	26.85889403964552	28.207665281089195	21.65002788021677
49	23.05541124196471	26.717914830321455	28.22008988802323	22.00658403969061
50	22.002876697299	28.203907839476084	27.817592742426378	21.97562272079854
51	21.93819860233192	28.21843661371346	27.03163615540578	22.811728628548842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	868.0
1	1516.0
2	2164.0
3	9076.0
4	15988.0
5	12361.5
6	8735.0
7	8185.5
8	7636.0
9	8130.0
10	8624.0
11	8928.0
12	9232.0
13	9099.0
14	8966.0
15	8781.5
16	8597.0
17	8497.5
18	8398.0
19	8611.5
20	8825.0
21	9473.0
22	10121.0
23	11076.5
24	12032.0
25	13615.0
26	17540.5
27	19883.0
28	23707.0
29	27531.0
30	31249.0
31	34967.0
32	40520.0
33	46073.0
34	53806.0
35	61539.0
36	64365.5
37	67192.0
38	74168.0
39	81144.0
40	92246.0
41	103348.0
42	112036.0
43	120724.0
44	126627.5
45	132531.0
46	131797.5
47	131064.0
48	133860.0
49	136656.0
50	135223.5
51	133791.0
52	131104.0
53	128417.0
54	122574.0
55	116731.0
56	111570.0
57	106409.0
58	101320.0
59	96231.0
60	91840.5
61	87450.0
62	78611.5
63	69773.0
64	62765.0
65	55757.0
66	46650.5
67	37544.0
68	31645.5
69	25747.0
70	21882.5
71	18018.0
72	15054.0
73	12090.0
74	10448.0
75	7257.0
76	5708.0
77	4399.5
78	3091.0
79	2260.5
80	1430.0
81	957.0
82	484.0
83	366.5
84	249.0
85	178.5
86	108.0
87	96.0
88	84.0
89	69.0
90	54.0
91	37.0
92	20.0
93	11.5
94	3.0
95	5.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1996039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.113884577777952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28992022523249	16.649526057309007
2	5.891951889782672	2.6058788805694935
3	2.245853440687803	1.4899363129792667
4	1.3642020937494432	1.206712305677528
5	1.0371398990696123	1.146759600951684
6	0.8003642457592263	1.06194975305399
7	0.6766712968830002	1.047468166945619
8	0.6066043259026772	1.0731502437914082
9	0.5620921952609309	1.1187037735263046
>10	10.508404936734745	57.030854704139614
>50	0.966010049285705	13.084939996234956
>100	0.048466436706419376	1.689772509389913
>500	0.0016232754616861708	0.22112506537762994
>1k	4.6379298905319165E-4	0.14897224372031886
>5k	2.3189649452659582E-4	0.4242503863332867
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8273	0.41447085953731366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01477927034491811	0.0
2	0.0	0.0	0.0	0.04899703863501665	0.0
3	0.0	0.0	0.0	0.07920686920445943	0.0
4	0.0	0.0	0.0	0.13211164711711545	0.0
5	0.0	0.0	0.0	0.2359673333036078	0.0
6	0.0	0.0	0.0	0.4122163945694448	0.0
7	0.0	0.0	0.0	0.5014932072970518	0.0
8	0.0	0.0	0.0	0.6622115098953477	0.0
9	0.0	0.0	0.0	0.752590505496135	0.0
10	0.0	0.0	0.0	0.9008842011603981	0.0
11	0.0	0.0	0.0	1.075029095122891	0.0
12	0.0	0.0	0.0	1.2049864757151538	0.0
13	0.0	0.0	0.0	1.2827905667173838	0.0
14	0.0	0.0	0.0	1.3311363154727938	0.0
15	0.0	0.0	0.0	1.384341688714499	0.0
16	0.0	0.0	0.0	1.4725163185689258	0.0
17	0.0	0.0	0.0	1.5773238899640738	0.0
18	0.0	0.0	0.0	1.6939548776351565	0.0
19	0.0	0.0	0.0	1.7658973597209273	0.0
20	0.0	0.0	0.0	1.850765440955813	0.0
21	0.0	0.0	0.0	1.9486092205613217	0.0
22	0.0	0.0	0.0	2.0629356440430273	0.0
23	0.0	0.0	0.0	2.1796668301571263	0.0
24	0.0	0.0	0.0	2.277510609762635	0.0
25	0.0	0.0	0.0	2.362829583991094	0.0
26	0.0	0.0	0.0	2.449150542649718	0.0
27	0.0	0.0	0.0	2.5424352930979803	0.0
28	0.0	0.0	0.0	2.6331649832493254	0.0
29	0.0	0.0	0.0	2.7421307900296537	0.0
30	0.0	0.0	0.0	2.865224577275294	0.0
31	0.0	0.0	0.0	2.976144253694442	0.0
32	0.0	0.0	0.0	3.0903203795116228	0.0
33	0.0	0.0	0.0	3.2059493827525416	0.0
34	0.0	0.0	0.0	3.3303958489789025	0.0
35	5.009922150819699E-5	0.0	0.0	3.46746731902533	0.0
36	5.009922150819699E-5	0.0	0.0	3.5948696393206747	0.0
37	1.0019844301639397E-4	0.0	0.0	3.7273319809883474	0.0
38	1.0019844301639397E-4	0.0	0.0	3.8761266688676925	0.0
39	1.0019844301639397E-4	0.0	0.0	4.101773562540612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	50	1.0822987E-9	40.500004	1
CGGTCTA	90	0.0	40.0	31
GGGCGAT	3400	0.0	38.977943	7
CGTTTTT	5760	0.0	38.632812	1
CACGACC	1025	0.0	38.414635	27
ACGGGAT	650	0.0	38.07692	5
AGGGCGA	1810	0.0	38.038677	6
CGTTAGG	290	0.0	38.01724	2
TACGGGA	790	0.0	37.87975	4
TAATACG	60	1.5643309E-10	37.500004	1
TAAGGGA	2645	0.0	37.344044	4
GTAGGGA	2865	0.0	37.225132	4
GGCGATA	865	0.0	37.196533	8
ACACGAC	1055	0.0	37.109005	26
TCGTTAG	85	0.0	37.058823	1
TTACGCG	85	0.0	37.058823	1
ACGACCA	1075	0.0	36.83721	28
GCGATCG	110	0.0	36.81818	9
TAGGGAC	2065	0.0	36.719128	5
TAGGGAG	2945	0.0	36.672325	5
>>END_MODULE