FastQCFastQC Report
Thu 26 May 2016
SRR1547992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547992_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2341721
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69110.29512482486171493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA11300.039.823014
AGGGCGA21500.039.6627926
CGTTTTT44300.039.260721
GGGCGAT42400.039.1627357
AAGGGAT41350.038.57925
GGCGATA9000.038.258
TACGTAG659.094947E-1238.0769271
TAGGGAC32500.038.0769235
TAAGGGA33650.037.711744
ACGGGCG3650.036.9862985
GTAGGGA40900.036.9682164
CTAGCGG4200.036.9642872
GCGATAC1400.036.9642839
ACGGGAT12800.036.738285
ATAGGGA35100.036.6666684
TCGATAG1600.036.56251
TAGGGAT38400.036.56255
ACACGAC5300.036.50943426
AAGGGAC29300.036.4761125
AACGGGA10250.036.4390264
Produced by FastQC (version 0.11.5)