FastQCFastQC Report
Thu 26 May 2016
SRR1547991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547991_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences928016
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60820.655376631437389No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14930.1608808468819503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG1250.045.00000426
TCGTTAG453.8380676E-1045.01
CGCATCG750.045.021
TGAACGA207.032585E-445.028
GCCGGAT253.8902235E-545.019
CGACCCA351.2115925E-745.020
CTTATCG351.2115925E-745.024
CTACGTG207.032585E-445.01
TGCGATC253.8902235E-545.022
CTAAACG207.032585E-445.01
ACGTCCC207.032585E-445.039
GTCCACC207.032585E-445.09
CGTCCCG253.8902235E-545.040
TATCGTG406.8121153E-945.01
AAGTGCG351.2115925E-745.01
TCGGTAC207.032585E-445.036
ATACCGG850.045.02
TGGTACG207.032585E-445.01
TACTCGC302.1650485E-644.99999645
TCCAACG302.1650485E-644.9999961