FastQCFastQC Report
Thu 26 May 2016
SRR1547990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547990_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2001663
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122650.6127405062690373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71750.041.64461
TAGTACG603.6379788E-1241.2500041
TACGGGA10850.041.0599064
CGCGTCA501.0822987E-940.539
AGGGCGA17850.039.957986
CGTAAGG4300.039.2441862
TAACGCG700.038.5714261
CGGTCTA700.038.57142631
CGCGTAA700.038.57142631
ACGGGAT11750.038.489365
GGGCGAT33050.038.396377
AGGGATC28650.038.3246086
ACGGGAC8750.038.0571445
AGGGATT46800.038.0288436
TAGGGAC28450.037.9613345
AAGGGAT44100.037.908165
TAGGGAT38100.037.8543325
TAAGGGA37750.037.7880824
TTAGGGA28200.037.6595734
GTAGGGA33400.037.5898174
Produced by FastQC (version 0.11.5)