##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547990_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2001663
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.876663054670043	31.0	31.0	31.0	30.0	33.0
2	31.207925609855405	31.0	31.0	33.0	30.0	34.0
3	31.311516973636422	31.0	31.0	34.0	30.0	34.0
4	35.231704337843084	37.0	35.0	37.0	33.0	37.0
5	35.09911958206751	37.0	35.0	37.0	32.0	37.0
6	35.150503356459105	37.0	35.0	37.0	32.0	37.0
7	35.550089100912594	36.0	35.0	37.0	35.0	37.0
8	35.64652241661059	37.0	35.0	37.0	35.0	37.0
9	37.47318055037236	39.0	37.0	39.0	35.0	39.0
10	36.59853232037561	39.0	35.0	39.0	32.0	39.0
11	36.189971039081	38.0	35.0	39.0	31.0	39.0
12	35.94300938769413	37.0	35.0	39.0	31.0	39.0
13	35.82672208058999	38.0	35.0	39.0	30.0	39.0
14	36.87352116714951	39.0	35.0	40.0	31.0	41.0
15	37.00871974952827	39.0	35.0	41.0	31.0	41.0
16	37.072509208593054	39.0	35.0	41.0	31.0	41.0
17	36.9894667583904	39.0	35.0	41.0	31.0	41.0
18	36.883940503471365	38.0	35.0	40.0	31.0	41.0
19	36.827174204648834	38.0	35.0	40.0	31.0	41.0
20	36.69137012574045	38.0	35.0	40.0	31.0	41.0
21	36.58657526266909	38.0	35.0	40.0	30.0	41.0
22	36.483227196586036	38.0	35.0	40.0	30.0	41.0
23	36.38543151369636	38.0	35.0	40.0	30.0	41.0
24	36.295122105968886	38.0	35.0	40.0	30.0	41.0
25	36.12379606357314	38.0	34.0	40.0	30.0	41.0
26	35.92291809360517	38.0	34.0	40.0	29.0	41.0
27	35.99071522029433	38.0	34.0	40.0	30.0	41.0
28	35.989685576443186	38.0	34.0	40.0	30.0	41.0
29	35.95421806767673	38.0	34.0	40.0	30.0	41.0
30	35.86198625842612	38.0	34.0	40.0	29.0	41.0
31	35.77407285841822	38.0	34.0	40.0	29.0	41.0
32	35.625502894343356	38.0	34.0	40.0	28.0	41.0
33	35.480402045699	38.0	34.0	40.0	27.0	41.0
34	35.301025697132836	38.0	34.0	40.0	26.0	41.0
35	35.146922334079214	38.0	34.0	40.0	25.0	41.0
36	35.06465673792242	38.0	34.0	40.0	25.0	41.0
37	35.01832626171338	38.0	34.0	40.0	25.0	41.0
38	34.92917039481671	38.0	34.0	40.0	24.0	41.0
39	34.89978882559152	38.0	34.0	40.0	24.0	41.0
40	34.73699718683914	38.0	33.0	40.0	24.0	41.0
41	34.65975791129676	38.0	33.0	40.0	24.0	41.0
42	34.62150571799549	38.0	33.0	40.0	24.0	41.0
43	34.5873686030066	38.0	33.0	40.0	23.0	41.0
44	34.447433958663375	37.0	33.0	40.0	23.0	41.0
45	34.32613032263673	37.0	33.0	40.0	23.0	41.0
46	34.25761629205316	37.0	33.0	40.0	23.0	41.0
47	34.213928118769246	37.0	33.0	40.0	23.0	41.0
48	34.12514743990372	37.0	33.0	40.0	23.0	41.0
49	33.98841263489408	37.0	33.0	40.0	23.0	41.0
50	33.88925208688975	37.0	33.0	40.0	23.0	41.0
51	33.55957371445643	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	3.0
13	6.0
14	20.0
15	70.0
16	197.0
17	539.0
18	1132.0
19	2088.0
20	3609.0
21	5699.0
22	8941.0
23	12941.0
24	18764.0
25	26790.0
26	34390.0
27	39462.0
28	44798.0
29	51516.0
30	60796.0
31	72775.0
32	88913.0
33	110047.0
34	153356.0
35	180357.0
36	176895.0
37	219822.0
38	304464.0
39	383222.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.72259116544593	25.410671027041015	28.028144597766957	13.838593209746097
2	32.14432199626011	26.33829970379629	28.633041625888072	12.884336674055522
3	28.319702167647602	26.402746116604042	31.952681345461247	13.324870370287107
4	24.842443508222914	28.956922319091678	31.268300408210575	14.932333764474839
5	22.640224653200864	32.87601359469601	30.278323573948263	14.205438178154864
6	21.32781592106164	42.19426546826314	26.67926619016288	9.798652420512344
7	90.38969097195681	2.6270156364982515	5.990718717386493	0.9925746741584373
8	90.89691921167548	2.1470147572293636	5.696563307609723	1.259502723485422
9	86.39691096852967	5.193481620032943	6.753984062252237	1.6556233491851524
10	36.83532143023076	41.13264820301919	12.227133138795091	9.804897227954955
11	25.236565795541004	27.162414452382844	29.945700150325006	17.655319601751142
12	26.388208204877646	25.3314868686687	30.336125511637075	17.94417941481658
13	22.951166105383376	28.414023739260802	30.85739207848674	17.777418076869083
14	18.803714711217623	31.64398802395808	30.841305454514572	18.710991810309725
15	18.217402230045717	28.915456797672732	35.50098093435309	17.366160037928463
16	22.19114806038779	27.669892484399224	33.378146071541515	16.760813383671476
17	21.56456906082592	26.642346888562162	30.062952654867477	21.73013139574444
18	22.20963269041792	27.29300586562273	31.695495195744737	18.801866248214612
19	23.868453381013687	29.00213472497618	28.524781644062962	18.604630249947167
20	25.543410654041164	28.696588786424087	28.70003592013241	17.059964639402338
21	24.168653764394907	27.64206562243495	30.56088862111155	17.628391992058603
22	23.205054996770187	24.769204406535966	30.963154137334808	21.062586459359043
23	20.098438148679374	29.553526242929006	30.515576298307955	19.832459310083667
24	19.836156236089693	28.740202521603287	33.16232552632486	18.26131571598216
25	20.951328969961477	29.751861327306344	30.315592584765767	18.981217117966413
26	20.453642796015114	31.819042466189362	28.219085830132247	19.508228907663277
27	19.457770863526978	30.655459985022453	30.94931564404198	18.937453507408588
28	18.580850023205706	30.087232466204348	32.61677914813833	18.715138362451622
29	19.322383438171162	28.51124290152738	32.45416436233272	19.712209297968737
30	20.834725925393034	28.30906101576539	32.00733590019899	18.848877158642587
31	22.89985876743488	28.754240848734273	29.12178523557662	19.224115148254224
32	22.05960743641662	28.956822402172595	29.60423407936301	19.379336082047775
33	21.706850753598385	28.987546854790242	29.103200688627407	20.20240170298397
34	19.76776310497821	29.756157754826862	30.418057385284136	20.05802175491079
35	19.98458281938568	29.62226908325727	30.314793249413114	20.078354847943935
36	22.57987483407547	29.847681652705777	28.017853155101534	19.554590358117224
37	20.447497905491584	30.936576236859054	28.70717997984676	19.90874587780261
38	20.346931526435768	31.698742495614894	28.240967635411156	19.71335834253818
39	20.411228063864897	29.992661102293443	28.50669668170916	21.089414152132502
40	21.950448202319773	28.966314509485365	28.981901548862123	20.101335739332747
41	19.364748211861837	29.521802621120543	29.18962882363315	21.923820343384477
42	20.90436801799304	30.10177037793075	27.683481185394342	21.310380418681866
43	20.496357278922574	29.62566625850605	28.363815487422205	21.514160975149164
44	19.900602648897443	30.290963064212107	29.26316767607734	20.54526661081311
45	20.244716518215103	30.84605150817096	28.01760336280383	20.891628610810113
46	20.91331058225086	30.758274494757607	28.09618801966165	20.23222690332988
47	20.13800524863576	30.35041363106577	28.91715538529713	20.594425735001344
48	20.673460017995037	29.64804764838037	29.71174468429501	19.966747649329584
49	20.896424622926038	29.03390830524419	29.312226883346497	20.757440188483276
50	19.57612245417935	30.65191293439505	29.398804893730862	20.373159717694737
51	19.608795286719094	30.751430185800505	28.81933672151606	20.82043780596434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	943.0
1	1434.5
2	1926.0
3	11416.0
4	20906.0
5	16166.5
6	11427.0
7	10774.0
8	10121.0
9	10778.5
10	11436.0
11	12154.0
12	12872.0
13	13037.0
14	13202.0
15	13055.5
16	12909.0
17	12646.0
18	12383.0
19	12135.0
20	11887.0
21	12553.5
22	13220.0
23	14605.0
24	15990.0
25	18998.0
26	26125.5
27	30245.0
28	35309.0
29	40373.0
30	45664.0
31	50955.0
32	57654.0
33	64353.0
34	73719.5
35	83086.0
36	88137.0
37	93188.0
38	102124.0
39	111060.0
40	119872.5
41	128685.0
42	135719.5
43	142754.0
44	146059.5
45	149365.0
46	150496.5
47	151628.0
48	151765.5
49	151903.0
50	143760.0
51	135617.0
52	128096.5
53	120576.0
54	109245.0
55	97914.0
56	87433.5
57	76953.0
58	69819.5
59	62686.0
60	55148.5
61	47611.0
62	40160.0
63	32709.0
64	26928.5
65	21148.0
66	17421.0
67	13694.0
68	11148.5
69	8603.0
70	6909.0
71	5215.0
72	4728.5
73	4242.0
74	3693.5
75	2191.0
76	1237.0
77	996.0
78	755.0
79	557.0
80	359.0
81	277.5
82	196.0
83	129.0
84	62.0
85	55.5
86	49.0
87	38.0
88	27.0
89	18.5
90	10.0
91	6.5
92	3.0
93	7.0
94	11.0
95	8.0
96	5.0
97	9.0
98	13.0
99	6.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2001663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.926724475722782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89197732860204	17.17027729654936
2	6.923019672932618	3.1744432916266914
3	2.8953999964645583	1.9914611389785495
4	1.7497484783726793	1.604640050618624
5	1.1880246430102082	1.361875683033198
6	0.9128679316999807	1.255744293168503
7	0.7476381824004955	1.1998626230798433
8	0.6452055208851605	1.1833959366039422
9	0.5577106979101051	1.150783155733324
>10	7.9171944763350055	42.951730554106895
>50	1.4507729890245427	22.0614291664278
>100	0.11731177325358277	3.7189376400096723
>500	0.0022345065064781895	0.33740022027716776
>1k	6.703519519434568E-4	0.20968404214917213
>5k	0.0	0.0
>10k+	2.2345065064781892E-4	0.628334907637331
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12265	0.6127405062690373	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.995845954089175E-5	0.0	0.0	0.019084131544620648	0.0
2	4.995845954089175E-5	0.0	0.0	0.05725239463386195	0.0
3	4.995845954089175E-5	0.0	0.0	0.09292273474605865	0.0
4	4.995845954089175E-5	0.0	0.0	0.1627147027246844	0.0
5	4.995845954089175E-5	0.0	0.0	0.29750262656601034	0.0
6	4.995845954089175E-5	0.0	0.0	0.5506421410597089	0.0
7	4.995845954089175E-5	0.0	0.0	0.6761877498859699	0.0
8	4.995845954089175E-5	0.0	0.0	0.9481116451670436	0.0
9	4.995845954089175E-5	0.0	0.0	1.0865465365548546	0.0
10	4.995845954089175E-5	0.0	0.0	1.2801355672758101	0.0
11	4.995845954089175E-5	0.0	0.0	1.4903607650238826	0.0
12	4.995845954089175E-5	0.0	0.0	1.6488289986875913	0.0
13	4.995845954089175E-5	0.0	0.0	1.7410023565405366	0.0
14	4.995845954089175E-5	0.0	0.0	1.7959566620355174	0.0
15	4.995845954089175E-5	0.0	0.0	1.8485129614725355	0.0
16	4.995845954089175E-5	0.0	0.0	1.9389877317010906	0.0
17	4.995845954089175E-5	0.0	0.0	2.0533926040497326	0.0
18	4.995845954089175E-5	0.0	0.0	2.183434474234674	0.0
19	4.995845954089175E-5	0.0	0.0	2.2700124846190395	0.0
20	4.995845954089175E-5	0.0	0.0	2.360587171766676	0.0
21	4.995845954089175E-5	0.0	0.0	2.473043664193223	0.0
22	4.995845954089175E-5	0.0	0.0	2.6105293448497573	0.0
23	4.995845954089175E-5	0.0	0.0	2.755109126761098	0.0
24	4.995845954089175E-5	0.0	0.0	2.8693641237311174	0.0
25	4.995845954089175E-5	0.0	0.0	2.9704300873823417	0.0
26	4.995845954089175E-5	0.0	0.0	3.0739440155510693	0.0
27	9.99169190817835E-5	0.0	0.0	3.1813547035639864	0.0
28	1.4987537862267526E-4	0.0	0.0	3.3024540094911083	0.0
29	1.4987537862267526E-4	0.0	0.0	3.433045422730999	0.0
30	1.4987537862267526E-4	0.0	0.0	3.590114819527563	0.0
31	1.4987537862267526E-4	0.0	0.0	3.730747883135173	0.0
32	1.4987537862267526E-4	0.0	0.0	3.8726798666908464	0.0
33	1.4987537862267526E-4	0.0	0.0	4.01126463345728	0.0
34	1.4987537862267526E-4	0.0	0.0	4.1520475724435135	0.0
35	1.4987537862267526E-4	0.0	0.0	4.319708162662746	0.0
36	1.4987537862267526E-4	0.0	0.0	4.473830010346397	0.0
37	1.4987537862267526E-4	0.0	0.0	4.6369443807474084	0.0
38	1.4987537862267526E-4	0.0	0.0	4.819542550369368	0.0
39	1.4987537862267526E-4	0.0	0.0	5.063289874469379	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7175	0.0	41.6446	1
TAGTACG	60	3.6379788E-12	41.250004	1
TACGGGA	1085	0.0	41.059906	4
CGCGTCA	50	1.0822987E-9	40.5	39
AGGGCGA	1785	0.0	39.95798	6
CGTAAGG	430	0.0	39.244186	2
TAACGCG	70	0.0	38.571426	1
CGGTCTA	70	0.0	38.571426	31
CGCGTAA	70	0.0	38.571426	31
ACGGGAT	1175	0.0	38.48936	5
GGGCGAT	3305	0.0	38.39637	7
AGGGATC	2865	0.0	38.324608	6
ACGGGAC	875	0.0	38.057144	5
AGGGATT	4680	0.0	38.028843	6
TAGGGAC	2845	0.0	37.961334	5
AAGGGAT	4410	0.0	37.90816	5
TAGGGAT	3810	0.0	37.854332	5
TAAGGGA	3775	0.0	37.788082	4
TTAGGGA	2820	0.0	37.659573	4
GTAGGGA	3340	0.0	37.589817	4
>>END_MODULE