FastQCFastQC Report
Thu 26 May 2016
SRR1547989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547989_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1969702
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63200.3208607190326252No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24050.12209968817618097No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20260.102858198854446No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20140.10224896964109292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAA253.8918224E-545.00000441
TAACGCG556.184564E-1140.9090921
CACGACG2450.040.40816526
ACGGGAT7500.040.25
TAGCCCG451.929584E-840.01
TCACGAC2500.039.60000225
CTCACGA2500.039.60000224
CGTTTTT41650.039.3277321
GTAGGGA27650.038.97834
ACGGGTA2550.038.823535
CGGGTAC1450.038.7931066
TACGGGA6200.038.467744
TAGGGAT31650.038.3175355
AGGGATT23500.037.5319146
CTAACGG900.037.52
CGGGTAT2400.037.4999966
TAAGGGA24050.037.422044
CGAATAT2650.037.3584914
TTAGGGA24950.037.3346674
CGGGATA3500.037.2857136
Produced by FastQC (version 0.11.5)