##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547989_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1969702
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.865999019140965	31.0	31.0	31.0	30.0	33.0
2	31.217569459745686	31.0	31.0	33.0	30.0	34.0
3	31.41322748314212	31.0	31.0	34.0	30.0	34.0
4	35.31556499409555	37.0	35.0	37.0	33.0	37.0
5	35.13878495325689	37.0	35.0	37.0	32.0	37.0
6	35.17581288946247	37.0	35.0	37.0	32.0	37.0
7	35.46539730375458	35.0	35.0	37.0	35.0	37.0
8	35.52715639218521	37.0	35.0	37.0	35.0	37.0
9	37.363773809439195	39.0	37.0	39.0	35.0	39.0
10	36.528716018971394	38.0	35.0	39.0	32.0	39.0
11	36.1289717937028	38.0	35.0	39.0	31.0	39.0
12	35.63936778253766	37.0	35.0	39.0	30.0	39.0
13	35.449866528033176	37.0	35.0	39.0	30.0	39.0
14	36.41839323918034	38.0	35.0	40.0	30.0	41.0
15	36.62512451122048	38.0	35.0	40.0	30.0	41.0
16	36.7541196587098	38.0	35.0	40.0	31.0	41.0
17	36.69299772249813	38.0	35.0	40.0	31.0	41.0
18	36.62922919304545	38.0	35.0	40.0	31.0	41.0
19	36.568045318530416	38.0	35.0	40.0	30.0	41.0
20	36.420292511252974	38.0	35.0	40.0	30.0	41.0
21	36.2890274772529	38.0	34.0	40.0	30.0	41.0
22	36.19938549080013	38.0	34.0	40.0	30.0	41.0
23	36.09720505944554	38.0	34.0	40.0	30.0	41.0
24	35.96377472328302	38.0	34.0	40.0	29.0	41.0
25	35.771468983633056	38.0	34.0	40.0	29.0	41.0
26	35.54111281808111	38.0	34.0	40.0	28.0	41.0
27	35.617488838413124	38.0	34.0	40.0	29.0	41.0
28	35.62061976887875	38.0	34.0	40.0	29.0	41.0
29	35.582615035167755	38.0	34.0	40.0	28.0	41.0
30	35.508231702054424	38.0	34.0	40.0	28.0	41.0
31	35.40089109926273	38.0	34.0	40.0	27.0	41.0
32	35.274344037829074	38.0	34.0	40.0	27.0	41.0
33	35.1406882868576	38.0	34.0	40.0	26.0	41.0
34	34.97347314466858	37.0	33.0	40.0	25.0	41.0
35	34.842746770831326	37.0	33.0	40.0	25.0	41.0
36	34.753875967024456	37.0	33.0	40.0	24.0	41.0
37	34.70608142754589	37.0	33.0	40.0	24.0	41.0
38	34.624402574602655	37.0	33.0	40.0	24.0	41.0
39	34.561464627644185	37.0	33.0	40.0	24.0	41.0
40	34.36126632353523	37.0	33.0	40.0	23.0	41.0
41	34.25238741697983	37.0	33.0	40.0	23.0	41.0
42	34.228629508423104	37.0	33.0	40.0	23.0	41.0
43	34.18083243048949	36.0	33.0	40.0	23.0	41.0
44	34.02355077062418	36.0	33.0	40.0	23.0	41.0
45	33.893269641803684	36.0	33.0	40.0	23.0	41.0
46	33.83475825277123	36.0	32.0	40.0	23.0	41.0
47	33.744390268172545	36.0	32.0	40.0	23.0	41.0
48	33.64537021336223	35.0	32.0	40.0	23.0	41.0
49	33.48722700185104	35.0	32.0	39.0	22.0	41.0
50	33.37313055477428	35.0	32.0	39.0	22.0	41.0
51	33.04398990304117	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	4.0
12	4.0
13	21.0
14	39.0
15	115.0
16	331.0
17	748.0
18	1536.0
19	2825.0
20	4645.0
21	7082.0
22	10326.0
23	14989.0
24	20369.0
25	27238.0
26	33862.0
27	40183.0
28	46177.0
29	54152.0
30	64457.0
31	77906.0
32	94426.0
33	117816.0
34	169420.0
35	187501.0
36	175602.0
37	214401.0
38	279916.0
39	323571.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.85466430962653	24.67378314079998	28.886755458439907	12.58479709113358
2	31.244421745015238	26.600521297130225	28.95808604550333	13.196970912351208
3	29.708148745343205	26.217265352830022	30.250971974440805	13.823613927385969
4	26.934683520654396	28.811667957894137	29.148114790968382	15.105533730483089
5	23.385111047254863	33.059975569908545	28.666925250621667	14.887988132214925
6	22.06734825877214	43.266037197505	24.51619585094598	10.150418692776878
7	90.95416464013338	3.091025952149107	4.6517696585574875	1.3030397491600252
8	91.14531030582292	2.7882898022137357	4.497685436680269	1.5687144552830834
9	86.81165983483797	5.020810254546119	5.690251621818935	2.4772782887969855
10	44.23669164167981	32.368500412752795	12.283939397939383	11.110868547628016
11	34.07957142755605	24.75460754977149	23.599255115748473	17.56656590692399
12	32.61772593011531	22.17492798403007	26.99169722120402	18.21564886465059
13	25.977584426476696	27.377136236852074	27.092169272306165	19.553110064365068
14	20.8701113163311	29.289912890376314	29.00926129942499	20.8307144938676
15	19.300635324531328	26.9032574470656	34.032254625318956	19.76385260308412
16	23.770854677509593	23.897269739280357	32.37804500376199	19.95383057944806
17	23.69972716685062	24.47547903185355	27.06810471837872	24.75668908291711
18	24.494568213871943	25.648854496771595	29.248739149373865	20.607838139982597
19	27.024697136927312	27.744704528908432	24.205590490338132	21.02500784382612
20	28.57158087873191	26.308243582024083	26.013224335457853	19.106951203786156
21	26.461617036485723	26.215996125302205	27.694747733413482	19.627639104798593
22	26.959712687502986	23.083339510240634	26.790651580797505	23.166296221458882
23	24.031503242622488	27.32687482675044	26.23325761968054	22.40836431094653
24	22.861529307479	26.527718406134532	30.604477225488928	20.006275060897536
25	23.924177362870118	27.50766359581297	27.030840198161954	21.537318843154953
26	23.226812989985287	28.900412346639236	25.14405732440745	22.72871733896803
27	21.743035240863847	28.979916758981815	27.90538873393031	21.37165926622403
28	21.023383232590515	27.406226931789686	29.910463613277543	21.659926222342264
29	21.861022631849895	26.185179280926757	29.263309881393223	22.69048820583012
30	22.83299707265363	27.604175657028325	28.449785805162403	21.113041465155643
31	26.15167167419234	27.506851290195165	25.597933088355497	20.743543947256995
32	27.097093875114105	26.656265770152032	25.199243337317018	21.04739701741685
33	26.085671842745757	27.084300061633687	24.990074640732455	21.8399534548881
34	22.44872574633117	26.984081856037108	27.3927731199948	23.174419277636922
35	23.28509591806273	26.985198776261587	27.744146068796194	21.98555923687949
36	27.276867262154376	27.0958754166874	24.636112467774314	20.991144853383915
37	22.814466350747473	29.0673919201991	26.394449515713546	21.723692213339884
38	23.39475717646629	28.32007075181931	26.599150531400177	21.686021540314222
39	22.403490477239703	28.2710785692455	26.171979314637444	23.153451638877353
40	25.438061188951426	25.75095115910935	26.965398826827613	21.845588825111616
41	21.664038519532397	25.518073292305132	28.76805730003828	24.049830888124195
42	22.889706158596578	26.430850961211394	26.104202564651914	24.575240315540118
43	22.695108194031384	25.927729169183966	27.07830930770238	24.298853329082267
44	21.83645038691132	27.1428368352167	27.786487499124235	23.23422527874775
45	21.71237070379174	28.608540784341997	26.03495351073411	23.644135001132152
46	23.22650837537861	28.06708832097444	26.165176255088333	22.541227048558614
47	22.534576296312846	27.201982838013063	27.909754876626007	22.35368598904809
48	22.762224945702446	26.796337720122132	28.377287528773387	22.064149805402035
49	22.62047761539563	26.66195190947666	27.885436477193	22.832133997934715
50	21.628957070663482	27.715969217678616	28.60843924613977	22.04663446551813
51	21.08374769381358	28.38825365461374	27.47618675312306	23.051811898449614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	950.0
1	1680.5
2	2411.0
3	7181.5
4	11952.0
5	9282.0
6	6612.0
7	6488.5
8	6365.0
9	6711.5
10	7058.0
11	7384.0
12	7710.0
13	7761.0
14	7812.0
15	7825.0
16	7838.0
17	7642.5
18	7447.0
19	7364.5
20	7282.0
21	7992.5
22	8703.0
23	9709.0
24	10715.0
25	12189.5
26	17141.5
27	20619.0
28	23025.5
29	25432.0
30	32144.5
31	38857.0
32	42305.0
33	45753.0
34	54162.5
35	62572.0
36	66257.5
37	69943.0
38	74457.5
39	78972.0
40	89318.0
41	99664.0
42	110284.5
43	120905.0
44	124444.0
45	127983.0
46	128088.5
47	128194.0
48	129533.0
49	130872.0
50	129427.5
51	127983.0
52	123266.5
53	118550.0
54	113038.5
55	107527.0
56	106116.0
57	104705.0
58	103058.0
59	101411.0
60	96102.5
61	90794.0
62	81134.5
63	71475.0
64	65750.0
65	60025.0
66	52271.0
67	44517.0
68	38388.5
69	32260.0
70	26949.0
71	21638.0
72	17774.5
73	13911.0
74	11182.5
75	6540.5
76	4627.0
77	3791.0
78	2955.0
79	2179.0
80	1403.0
81	977.5
82	552.0
83	434.0
84	316.0
85	232.0
86	148.0
87	126.0
88	104.0
89	70.5
90	37.0
91	27.5
92	18.0
93	12.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1969702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.17658047479226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68705359774818	15.674590887644657
2	6.111584714500476	2.466217616412583
3	2.1973103328326262	1.3300262627547015
4	1.3272767606607216	1.0711962549517056
5	0.8935143249512634	0.9014031841379425
6	0.6936519002982231	0.8397314032715807
7	0.5906980110515991	0.8342786169397622
8	0.4895574112472459	0.7902075604048822
9	0.41502635581869035	0.7536431400602016
>10	7.0578251230177464	36.11159047978225
>50	2.288092218494025	31.672632069398777
>100	0.2440331280102251	6.305417569956062
>500	0.0025741890405189486	0.3710474461895156
>1k	0.0015445134243113694	0.5497672477486034
>5k	2.5741890405189485E-4	0.3282502603468279
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6320	0.3208607190326252	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2405	0.12209968817618097	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2026	0.102858198854446	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2014	0.10224896964109292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0153820222551432E-4	0.0	0.0	0.012641506177076533	0.0
2	1.0153820222551432E-4	0.0	0.0	0.04157989381134811	0.0
3	1.0153820222551432E-4	0.0	0.0	0.06797982638998183	0.0
4	1.0153820222551432E-4	0.0	0.0	0.12509506514183363	0.0
5	1.0153820222551432E-4	0.0	0.0	0.2198302078182385	0.0
6	1.0153820222551432E-4	0.0	0.0	0.3910743858715684	0.0
7	1.0153820222551432E-4	0.0	0.0	0.47372648248313703	0.0
8	1.0153820222551432E-4	0.0	0.0	0.6623844622181426	0.0
9	1.0153820222551432E-4	0.0	0.0	0.7514842346710314	0.0
10	1.0153820222551432E-4	0.0	0.0	0.8934854104834132	0.0
11	1.0153820222551432E-4	0.0	0.0	1.0804680098816979	0.0
12	1.0153820222551432E-4	0.0	0.0	1.217392275582804	0.0
13	1.0153820222551432E-4	0.0	0.0	1.2902967047807232	0.0
14	1.0153820222551432E-4	0.0	0.0	1.3350242828610623	0.0
15	1.0153820222551432E-4	0.0	0.0	1.3813764721770094	0.0
16	1.0153820222551432E-4	0.0	0.0	1.4637239541819016	0.0
17	1.0153820222551432E-4	0.0	0.0	1.554803721578188	0.0
18	1.0153820222551432E-4	0.0	0.0	1.6603019136904973	0.0
19	1.0153820222551432E-4	0.0	0.0	1.7293986603049598	0.0
20	1.0153820222551432E-4	0.0	0.0	1.8007292473683836	0.0
21	1.0153820222551432E-4	0.0	0.0	1.893941317011406	0.0
22	1.0153820222551432E-4	0.0	0.0	1.9962918248547241	0.0
23	1.0153820222551432E-4	0.0	0.0	2.1051915467415885	0.0
24	1.0153820222551432E-4	0.0	0.0	2.191549787734388	0.0
25	1.0153820222551432E-4	0.0	0.0	2.2744049607504078	0.0
26	1.0153820222551432E-4	0.0	0.0	2.3508632270262204	0.0
27	1.0153820222551432E-4	0.0	0.0	2.4374245444234712	0.0
28	1.0153820222551432E-4	0.0	0.0	2.5193151045183484	0.0
29	1.0153820222551432E-4	0.0	0.0	2.616842547755955	0.0
30	1.0153820222551432E-4	0.0	0.0	2.7428006876167053	0.0
31	1.0153820222551432E-4	0.0	0.0	2.8498219527623974	0.0
32	1.0153820222551432E-4	0.0	0.0	2.9518678459990393	0.0
33	1.0153820222551432E-4	0.0	0.0	3.049141443731082	0.0
34	1.0153820222551432E-4	0.0	0.0	3.163168844830335	0.0
35	1.0153820222551432E-4	0.0	0.0	3.3133438459218705	0.0
36	1.0153820222551432E-4	0.0	0.0	3.4269650942122207	0.0
37	1.0153820222551432E-4	0.0	0.0	3.550943239129574	0.0
38	1.0153820222551432E-4	0.0	0.0	3.7068043795457384	0.0
39	1.0153820222551432E-4	0.0	0.0	3.9824805985880096	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAA	25	3.8918224E-5	45.000004	41
TAACGCG	55	6.184564E-11	40.909092	1
CACGACG	245	0.0	40.408165	26
ACGGGAT	750	0.0	40.2	5
TAGCCCG	45	1.929584E-8	40.0	1
TCACGAC	250	0.0	39.600002	25
CTCACGA	250	0.0	39.600002	24
CGTTTTT	4165	0.0	39.327732	1
GTAGGGA	2765	0.0	38.9783	4
ACGGGTA	255	0.0	38.82353	5
CGGGTAC	145	0.0	38.793106	6
TACGGGA	620	0.0	38.46774	4
TAGGGAT	3165	0.0	38.317535	5
AGGGATT	2350	0.0	37.531914	6
CTAACGG	90	0.0	37.5	2
CGGGTAT	240	0.0	37.499996	6
TAAGGGA	2405	0.0	37.42204	4
CGAATAT	265	0.0	37.35849	14
TTAGGGA	2495	0.0	37.334667	4
CGGGATA	350	0.0	37.285713	6
>>END_MODULE