FastQCFastQC Report
Thu 26 May 2016
SRR1547988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547988_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2347037
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131250.5592157260409615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG351.2126475E-745.0000041
TAGGACG800.042.18751
CGTTTTT83500.041.4700621
TTTCGCG603.6379788E-1241.2499961
TATAGCG1500.040.51
TACGGGA13800.040.2717364
TAGGGAC32650.039.900465
GCGCGAC4250.039.7058839
CCGAACG403.4602817E-739.3751
CGTAAGG3100.039.1935462
AGGGCGA18250.038.589046
CGCGAAA356.2510717E-638.5714323
GGGCGAT35350.038.507787
CGGGGTA4800.038.4374966
ATAGGGA41250.038.2363624
CGTTAGG4650.038.2258032
TTAGGGA35350.037.9349374
AGGGATC35350.037.9349376
GAGGGAT43950.037.883965
CGAGGGA16850.037.7893184
Produced by FastQC (version 0.11.5)