##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547988_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2347037
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.856532726156427	31.0	31.0	31.0	30.0	33.0
2	31.192249206126704	31.0	31.0	33.0	30.0	34.0
3	31.30198970020498	31.0	31.0	34.0	30.0	34.0
4	35.20882201686637	37.0	35.0	37.0	33.0	37.0
5	35.061607039002794	35.0	35.0	37.0	32.0	37.0
6	35.111158452124954	37.0	35.0	37.0	32.0	37.0
7	35.54992784519375	35.0	35.0	37.0	35.0	37.0
8	35.64339377691958	37.0	35.0	37.0	35.0	37.0
9	37.462117981097016	39.0	37.0	39.0	35.0	39.0
10	36.573565734157576	39.0	35.0	39.0	32.0	39.0
11	36.137296514711956	38.0	35.0	39.0	31.0	39.0
12	35.90545781766542	37.0	35.0	39.0	31.0	39.0
13	35.789411500543025	38.0	35.0	39.0	30.0	39.0
14	36.83982655578075	39.0	35.0	40.0	31.0	41.0
15	36.97727560323932	39.0	35.0	41.0	31.0	41.0
16	37.01976832917419	39.0	35.0	41.0	31.0	41.0
17	36.935334636820805	38.0	35.0	40.0	31.0	41.0
18	36.82866184043967	38.0	35.0	40.0	31.0	41.0
19	36.78084410258551	38.0	35.0	40.0	31.0	41.0
20	36.65200932068817	38.0	35.0	40.0	31.0	41.0
21	36.544247917693674	38.0	35.0	40.0	30.0	41.0
22	36.42452803257895	38.0	35.0	40.0	30.0	41.0
23	36.32749547621107	38.0	35.0	40.0	30.0	41.0
24	36.23481606808925	38.0	34.0	40.0	30.0	41.0
25	36.05963178254114	38.0	34.0	40.0	30.0	41.0
26	35.84439955569512	38.0	34.0	40.0	29.0	41.0
27	35.909495248690156	38.0	34.0	40.0	29.0	41.0
28	35.92914172209471	38.0	34.0	40.0	29.0	41.0
29	35.90617659627863	38.0	34.0	40.0	29.0	41.0
30	35.811904541769046	38.0	34.0	40.0	29.0	41.0
31	35.73771269903287	38.0	34.0	40.0	29.0	41.0
32	35.58477859530974	38.0	34.0	40.0	28.0	41.0
33	35.41809609307395	38.0	34.0	40.0	27.0	41.0
34	35.254726278281936	38.0	34.0	40.0	26.0	41.0
35	35.10007000315717	38.0	34.0	40.0	25.0	41.0
36	35.04236320092099	38.0	34.0	40.0	25.0	41.0
37	35.00398161596941	38.0	34.0	40.0	25.0	41.0
38	34.92010309168539	38.0	34.0	40.0	24.0	41.0
39	34.873612985223495	38.0	34.0	40.0	24.0	41.0
40	34.721277508620446	38.0	33.0	40.0	24.0	41.0
41	34.651155052093344	38.0	33.0	40.0	24.0	41.0
42	34.61540231364056	38.0	33.0	40.0	24.0	41.0
43	34.57763725071228	38.0	33.0	40.0	24.0	41.0
44	34.433655711435314	37.0	33.0	40.0	23.0	41.0
45	34.30437185268064	37.0	33.0	40.0	23.0	41.0
46	34.23282078637874	37.0	33.0	40.0	23.0	41.0
47	34.19345412961108	37.0	33.0	40.0	23.0	41.0
48	34.085293925915956	37.0	33.0	40.0	23.0	41.0
49	33.9523407598602	37.0	33.0	40.0	23.0	41.0
50	33.86332980690121	37.0	33.0	40.0	22.0	41.0
51	33.53439847774023	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	6.0
14	26.0
15	82.0
16	264.0
17	595.0
18	1341.0
19	2468.0
20	4316.0
21	6776.0
22	10401.0
23	15165.0
24	22303.0
25	31258.0
26	40456.0
27	46926.0
28	53110.0
29	61673.0
30	72838.0
31	87511.0
32	106318.0
33	130387.0
34	181199.0
35	209640.0
36	208123.0
37	256076.0
38	353629.0
39	444115.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.66000365567309	25.221204437765575	29.131070366594138	13.987721539967202
2	30.928996858592345	26.936558733415794	29.01049280433159	13.123951603660275
3	28.283107594809962	27.03587544636067	31.641725290227633	13.039291668601729
4	25.10757180223405	28.935547245313987	31.442793615950666	14.5140873365013
5	23.108966752548	33.364748830120696	29.65040602257229	13.875878394759008
6	21.198856260041918	42.152424525050094	26.38266887143236	10.266050343475625
7	90.69720673342601	2.534983470648311	5.805830926397837	0.9619788695278344
8	91.14398281748434	2.1196086810732	5.571791156253608	1.1646173451888489
9	86.39177822931636	5.149045370822872	6.631808531352509	1.827367868508251
10	37.19025307227794	42.04164655265341	11.632496632988742	9.135603742079908
11	24.589514353629703	27.449205104137686	30.08337746699349	17.87790307523912
12	26.286803318396768	25.07003511235656	30.242386464295194	18.400775104951478
13	23.517907898341612	27.357600242348116	30.50041392615455	18.624077933155718
14	18.26715982747609	31.776789202726675	30.661553269079267	19.29449770071797
15	18.508613200388403	29.6323832986016	34.64244492097909	17.21655858003091
16	22.568285033427255	27.646517715741165	33.41498237991135	16.370214870920226
17	22.198755281659384	26.899831574875044	29.76361258897921	21.137800554486358
18	21.961051317043577	27.98626523569931	31.447736017796057	18.60494742946106
19	24.122329558502912	28.93605852826351	28.381486955680717	18.560124957552866
20	25.81821249515879	28.94594333195429	28.27220022522014	16.96364394766678
21	24.84272723438105	27.778258289068305	30.081502762845236	17.297511713705408
22	23.389533271098838	25.14617366492305	30.348349855583873	21.115943208394246
23	20.542880235803697	29.202692586439838	30.3729766509859	19.88145052677056
24	20.778326034059113	28.523197546523555	32.43391561360132	18.264560805816014
25	21.082326354463095	29.850019407448624	29.96305554620571	19.104598691882575
26	20.648204523405468	32.22492870798372	27.880046202935873	19.246820565674934
27	19.49202334688375	31.127885925956853	30.58865284185976	18.791437885299636
28	18.898210807925057	30.179796909891067	32.35368679743864	18.568305484745235
29	19.683584025305098	28.972444831504575	31.680412366741557	19.663558776448774
30	20.481568888773378	28.822724141119206	31.282463804362692	19.413243165744724
31	22.648172994290245	28.99920197252962	28.722384862275284	19.630240170904848
32	22.80522207361878	29.4870937271121	28.830265564624675	18.877418634644446
33	22.034122171912927	29.334177518292215	28.646587165008476	19.985113144786386
34	20.520895068974202	29.429233539991063	29.884062330504374	20.165809060530364
35	20.22533091723735	28.946071152691673	30.009795329174615	20.818802600896362
36	22.276086827774765	29.615979637304395	28.598867423052983	19.509066111867856
37	20.437385520552084	30.727508769567756	29.008405065621034	19.826700644259123
38	20.83316113039547	30.876334714791458	27.924570426456846	20.365933728356218
39	20.68250308793598	29.85070111804799	28.590942537335373	20.875853256680657
40	22.027305065919283	28.71637728761839	29.008490279445958	20.24782736701637
41	19.52091083353181	29.08658874998562	29.562252320692007	21.830248095790562
42	20.79566704743044	29.827352530019763	28.328185708192926	21.048794714356866
43	20.721701447399425	28.942364351307624	28.66580288252806	21.67013131876489
44	20.615013738598922	29.869916835567565	29.080325533853962	20.434743891979547
45	20.608367060255123	30.194368473952476	28.166492475406223	21.030771990386178
46	21.19306171994732	30.5344568492103	27.982941896527407	20.28953953431497
47	20.68271612249828	29.554412648799318	28.79907730470376	20.96379392399864
48	20.442498350047313	29.18198562698415	29.945331070622235	20.4301849523463
49	21.020801972870476	28.458818501796095	29.717085840572604	20.803293684760828
50	19.57595896443047	30.121297619083126	29.457183674564995	20.84555974192141
51	19.442514114604926	29.99564983423781	29.025746078992366	21.536089972164906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1196.0
1	1760.0
2	2324.0
3	12774.5
4	23225.0
5	18015.5
6	12806.0
7	12155.5
8	11505.0
9	12255.5
10	13006.0
11	13514.0
12	14022.0
13	14152.0
14	14282.0
15	14277.0
16	14272.0
17	13593.0
18	12914.0
19	13090.0
20	13266.0
21	14318.0
22	15370.0
23	17363.0
24	19356.0
25	22392.0
26	29793.5
27	34159.0
28	39745.5
29	45332.0
30	52324.5
31	59317.0
32	68279.0
33	77241.0
34	87724.0
35	98207.0
36	103361.5
37	108516.0
38	117257.5
39	125999.0
40	136942.5
41	147886.0
42	157036.0
43	166186.0
44	170191.0
45	174196.0
46	179833.0
47	185470.0
48	185897.0
49	186324.0
50	173252.5
51	160181.0
52	147451.0
53	134721.0
54	123602.0
55	112483.0
56	101724.5
57	90966.0
58	83980.0
59	76994.0
60	67880.5
61	58767.0
62	51450.0
63	44133.0
64	35819.5
65	27506.0
66	21119.0
67	14732.0
68	11481.0
69	8230.0
70	7081.0
71	5932.0
72	5020.0
73	4108.0
74	3606.5
75	2475.0
76	1845.0
77	1283.5
78	722.0
79	579.0
80	436.0
81	323.0
82	210.0
83	149.0
84	88.0
85	60.0
86	32.0
87	26.0
88	20.0
89	16.0
90	12.0
91	9.5
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2347037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.063024550202993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.753998519179	17.471163276238666
2	6.936210635250129	3.1993999233230563
3	2.829421282425983	1.9576503749837184
4	1.654750618000466	1.526542165096333
5	1.1117268591138056	1.2819891922430882
6	0.8279560100122145	1.145710187123989
7	0.7115142273142641	1.1486769064657307
8	0.5778915277270689	1.0662341193058962
9	0.499142936539054	1.0360571219514554
>10	7.404458159985318	40.74025728346525
>50	1.5496447242079814	24.06721436737202
>100	0.14119695587508974	4.356155382764233
>500	0.0011386605652164137	0.17078375422647185
>1k	7.591070434776092E-4	0.257707520472954
>5k	0.0	0.0
>10k+	1.897767608694023E-4	0.5744584249673099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13125	0.5592157260409615	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020749566368148436	0.0
2	0.0	0.0	0.0	0.07319867560673309	0.0
3	0.0	0.0	0.0	0.12172794889897347	0.0
4	0.0	0.0	0.0	0.20728262911918305	0.0
5	0.0	0.0	0.0	0.39155752550982365	0.0
6	0.0	0.0	0.0	0.7046757251803017	0.0
7	0.0	0.0	0.0	0.8637699363069266	0.0
8	0.0	0.0	0.0	1.162870461777978	0.0
9	0.0	0.0	0.0	1.3063705429441461	0.0
10	0.0	0.0	0.0	1.5253274660774414	0.0
11	0.0	0.0	0.0	1.7921745588160731	0.0
12	0.0	0.0	0.0	1.9951538897767696	0.0
13	0.0	0.0	0.0	2.1137289271536837	0.0
14	0.0	0.0	0.0	2.1824112700396285	0.0
15	0.0	0.0	0.0	2.2578255050942957	0.0
16	0.0	0.0	0.0	2.3799369162054114	0.0
17	0.0	0.0	0.0	2.5331087665000593	0.0
18	0.0	0.0	0.0	2.717085414503478	0.0
19	0.0	0.0	0.0	2.842392344049114	0.0
20	0.0	0.0	0.0	2.9654411072343554	0.0
21	0.0	0.0	0.0	3.117590391629957	0.0
22	0.0	0.0	0.0	3.2980306658991743	0.0
23	0.0	0.0	0.0	3.4908269447818676	0.0
24	0.0	0.0	0.0	3.642464946227946	0.0
25	0.0	0.0	0.0	3.77237342231929	0.0
26	0.0	0.0	0.0	3.899427235275797	0.0
27	0.0	0.0	0.0	4.036664100310306	0.0
28	0.0	0.0	0.0	4.182379740924408	0.0
29	0.0	0.0	0.0	4.3487171271692775	0.0
30	0.0	0.0	0.0	4.539340453516497	0.0
31	0.0	0.0	0.0	4.709043785845728	0.0
32	0.0	0.0	0.0	4.8907622674887525	0.0
33	0.0	0.0	0.0	5.066174926087658	0.0
34	0.0	0.0	0.0	5.24414399943418	0.0
35	0.0	0.0	0.0	5.440647079700916	0.0
36	0.0	0.0	0.0	5.6257741143407625	0.0
37	0.0	0.0	0.0	5.815289660964016	0.0
38	0.0	0.0	0.0	6.018439419574553	0.0
39	0.0	0.0	0.0	6.258188515988457	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	35	1.2126475E-7	45.000004	1
TAGGACG	80	0.0	42.1875	1
CGTTTTT	8350	0.0	41.470062	1
TTTCGCG	60	3.6379788E-12	41.249996	1
TATAGCG	150	0.0	40.5	1
TACGGGA	1380	0.0	40.271736	4
TAGGGAC	3265	0.0	39.90046	5
GCGCGAC	425	0.0	39.705883	9
CCGAACG	40	3.4602817E-7	39.375	1
CGTAAGG	310	0.0	39.193546	2
AGGGCGA	1825	0.0	38.58904	6
CGCGAAA	35	6.2510717E-6	38.57143	23
GGGCGAT	3535	0.0	38.50778	7
CGGGGTA	480	0.0	38.437496	6
ATAGGGA	4125	0.0	38.236362	4
CGTTAGG	465	0.0	38.225803	2
TTAGGGA	3535	0.0	37.934937	4
AGGGATC	3535	0.0	37.934937	6
GAGGGAT	4395	0.0	37.88396	5
CGAGGGA	1685	0.0	37.789318	4
>>END_MODULE