FastQCFastQC Report
Thu 26 May 2016
SRR1547987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547987_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1782252
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79980.4487580880818201No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG351.2124292E-745.0000041
ACGTGCG302.166178E-645.0000041
TATTACG351.2124292E-745.0000041
TTCGGTC207.034333E-445.033
TCGCGAT207.034333E-445.040
CGACGGT1400.041.78571728
CGTTTTT51050.041.6062661
TATACGG1300.041.538462
TACGACG451.929402E-840.01
TAGGGCG6850.039.7445265
ACGGGAC5550.039.729735
GTACGTC403.459645E-739.37515
AGGGCGA15300.038.970596
TAAGGGA25250.038.673274
CGGGATC3550.038.6619726
GCCGATC356.250224E-638.571439
GGGCGAT29200.038.5273977
TGGGCGA8800.038.3522726
ACGGGTC1900.037.8947375
ATCGCCA950.037.89473724
Produced by FastQC (version 0.11.5)