##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547987_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1782252
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.786574513592914	31.0	31.0	31.0	30.0	33.0
2	31.118534303790934	31.0	31.0	33.0	30.0	34.0
3	31.26175815765672	31.0	31.0	34.0	28.0	34.0
4	35.160607478628165	35.0	35.0	37.0	33.0	37.0
5	34.9871740921037	35.0	35.0	37.0	32.0	37.0
6	35.026923802021265	36.0	35.0	37.0	32.0	37.0
7	35.4826326467862	35.0	35.0	37.0	35.0	37.0
8	35.58180324667892	37.0	35.0	37.0	35.0	37.0
9	37.407094507398504	39.0	37.0	39.0	35.0	39.0
10	36.43141261729542	38.0	35.0	39.0	32.0	39.0
11	35.991046159577884	38.0	35.0	39.0	31.0	39.0
12	35.63578158419797	37.0	35.0	39.0	30.0	39.0
13	35.472732251107026	37.0	35.0	39.0	30.0	39.0
14	36.51239274805134	38.0	35.0	40.0	30.0	41.0
15	36.69393273229599	38.0	35.0	40.0	31.0	41.0
16	36.78356932689653	38.0	35.0	40.0	31.0	41.0
17	36.71203118302014	38.0	35.0	40.0	31.0	41.0
18	36.63638868128637	38.0	35.0	40.0	31.0	41.0
19	36.56461207506009	38.0	35.0	40.0	30.0	41.0
20	36.452598173546725	38.0	35.0	40.0	30.0	41.0
21	36.334552016213195	38.0	34.0	40.0	30.0	41.0
22	36.207159256940095	38.0	34.0	40.0	30.0	41.0
23	36.130856354769136	38.0	34.0	40.0	30.0	41.0
24	36.031980326014505	38.0	34.0	40.0	30.0	41.0
25	35.834227707417355	38.0	34.0	40.0	29.0	41.0
26	35.59232196120414	38.0	34.0	40.0	29.0	41.0
27	35.65802086349181	38.0	34.0	40.0	29.0	41.0
28	35.691444307539	38.0	34.0	40.0	29.0	41.0
29	35.656923375594474	38.0	34.0	40.0	29.0	41.0
30	35.59457809557795	38.0	34.0	40.0	29.0	41.0
31	35.49680348233583	38.0	34.0	40.0	28.0	41.0
32	35.355660703424654	38.0	34.0	40.0	27.0	41.0
33	35.20357502754941	38.0	34.0	40.0	27.0	41.0
34	35.06519616754533	38.0	34.0	40.0	26.0	41.0
35	34.913942584999205	38.0	33.0	40.0	25.0	41.0
36	34.830079584705196	38.0	33.0	40.0	25.0	41.0
37	34.75951941700725	38.0	33.0	40.0	24.0	41.0
38	34.68974140581691	37.0	33.0	40.0	24.0	41.0
39	34.66399196073282	37.0	33.0	40.0	24.0	41.0
40	34.50596492527431	37.0	33.0	40.0	24.0	41.0
41	34.449390995212795	37.0	33.0	40.0	23.0	41.0
42	34.3992763088497	37.0	33.0	40.0	23.0	41.0
43	34.35132854388717	37.0	33.0	40.0	23.0	41.0
44	34.20755931260001	37.0	33.0	40.0	23.0	41.0
45	34.06842017851572	37.0	33.0	40.0	23.0	41.0
46	34.01578340212271	37.0	33.0	40.0	23.0	41.0
47	33.972823708431804	36.0	33.0	40.0	23.0	41.0
48	33.87383602318864	36.0	33.0	40.0	23.0	41.0
49	33.719307931762735	36.0	32.0	40.0	22.0	41.0
50	33.59020553771296	36.0	32.0	40.0	22.0	41.0
51	33.2553611947132	35.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	5.0
14	30.0
15	74.0
16	178.0
17	467.0
18	1092.0
19	2033.0
20	3525.0
21	5598.0
22	8505.0
23	12418.0
24	17917.0
25	24800.0
26	31389.0
27	36711.0
28	41609.0
29	49495.0
30	58900.0
31	70646.0
32	84965.0
33	105255.0
34	148320.0
35	173087.0
36	157623.0
37	188393.0
38	253950.0
39	305235.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15597345381012	25.2559121830134	29.35022656728678	12.237887795889694
2	30.811074977051504	27.319930066006375	28.80512968985306	13.063865267089053
3	28.721976465729878	26.052095887674692	31.246310847175373	13.979616799420057
4	24.71395739771929	28.978113083896105	31.236379591662683	15.071549926721922
5	22.720636587867485	33.52477651869657	29.93970549619246	13.814881397243486
6	20.789428206561137	42.95223122207185	26.73875523775538	9.519585333611632
7	90.9493158094366	3.0468194172316823	5.028918469442032	0.9749463038896856
8	91.71890394848764	2.331825129106322	4.612759587308641	1.3365113350973936
9	87.46198629598958	5.116672614198216	5.726238489282099	1.695102600530116
10	41.25286435363798	33.74455464210448	13.6500337774905	11.352547226767035
11	29.183359031158336	27.990317867506953	25.147566112985142	17.67875698834957
12	28.368378882447598	25.289072476843906	28.37903955220698	17.963509088501514
13	23.024746219950938	31.154685196032883	28.290331558051275	17.530237025964905
14	19.158401842163734	34.799848730707	27.89252024966166	18.149229177467607
15	18.420964038755464	29.77614837856824	35.16478028920714	16.63810729346916
16	20.705994438496912	27.27835345394479	34.44271629376766	17.57293581379064
17	20.687422429600304	27.730983048412906	28.243480719898194	23.338113802088596
18	21.863687065577707	28.58838144100834	30.599208192780818	18.948723300633134
19	24.876448448367572	30.33987337368678	26.245446771836978	18.538231406108675
20	26.392900667245712	28.7476181819406	26.766472979129773	18.093008171683913
21	23.734410173196608	29.259386439179195	28.448333905642976	18.557869481981225
22	23.23666911300983	27.036019597677548	27.70186258733333	22.025448701979293
23	20.354304554013687	31.953954884045576	27.24581035678456	20.445930205156174
24	20.220148441410082	29.664211346094717	32.01769446744905	18.097945745046154
25	20.61717422676479	29.889039260441287	30.064603658741863	19.429182854052065
26	20.210553838626634	34.15145557418368	26.86679549244439	18.7711950947453
27	19.374140132820724	33.5835785287378	28.397499343527176	18.6447819949143
28	18.96645367770663	31.168544066720084	31.691253537659097	18.17374871791419
29	19.086582593258417	29.752526578732976	31.019799669182586	20.141091158826026
30	20.145411535517983	31.071812515850734	29.83882189499577	18.94395405363551
31	23.43978292632018	30.44075697488346	27.14952767622087	18.969932422575482
32	23.530454728063148	31.35645239842626	26.87397741733492	18.239115456175668
33	23.215011120761822	31.19233419291997	26.051927561310073	19.540727125008136
34	20.249886099159937	31.348765494441867	27.818119996498815	20.583228409899384
35	20.697129249960163	30.577550200532812	28.25964005090189	20.465680498605135
36	23.801067413586853	30.64555405184003	26.554830630011917	18.998547904561196
37	20.57879581563101	33.355440195887006	27.298159856181954	18.767604132300033
38	20.829882642858585	33.249562912539865	26.11106622408055	19.809488220521004
39	20.625253892266638	32.409221591559444	26.730282810736078	20.23524170543784
40	22.37859741495591	29.963944492698	27.063344577534494	20.5941135148116
41	19.2105830151965	29.50076644604691	28.426395369453928	22.86225516930266
42	21.431481070017032	30.40619396134778	26.27080794410667	21.891517024528518
43	20.983732940122945	30.57878459387337	26.96874516061702	21.468737305386668
44	20.134077560300113	32.30311987305948	27.6577049710142	19.90509759562621
45	20.03044462848127	33.47865509479019	25.579715999757607	20.911184276970936
46	21.163112736021617	32.64605678658236	26.255546353714287	19.935284123681722
47	21.296876087107773	30.947194897242365	27.07630570761037	20.679623308039492
48	21.33587169491183	30.102701525934606	28.27417222704758	20.28725455210599
49	20.83678402380808	29.831864405258067	28.646959015896744	20.68439255503711
50	19.899318390440857	32.16716827923324	27.714992043773833	20.218521286552072
51	19.439899632599655	32.26075773796298	26.727533480113923	21.57180914932344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	936.0
1	1286.5
2	1637.0
3	7383.5
4	13130.0
5	10335.5
6	7541.0
7	7402.5
8	7264.0
9	8330.0
10	9396.0
11	10499.5
12	11603.0
13	11718.0
14	11833.0
15	11533.5
16	11234.0
17	11134.0
18	11034.0
19	10643.5
20	10253.0
21	10805.0
22	11357.0
23	12239.0
24	13121.0
25	14570.0
26	19731.0
27	23443.0
28	27307.0
29	31171.0
30	35522.5
31	39874.0
32	47434.0
33	54994.0
34	61545.0
35	68096.0
36	73274.5
37	78453.0
38	85326.0
39	92199.0
40	104507.5
41	116816.0
42	129632.5
43	142449.0
44	140826.5
45	139204.0
46	143487.5
47	147771.0
48	145902.5
49	144034.0
50	136464.5
51	128895.0
52	119085.5
53	109276.0
54	97839.0
55	86402.0
56	78264.5
57	70127.0
58	63221.5
59	56316.0
60	49885.0
61	43454.0
62	36548.0
63	29642.0
64	23372.0
65	17102.0
66	13470.5
67	9839.0
68	8087.0
69	6335.0
70	5068.0
71	3801.0
72	3177.0
73	2553.0
74	2129.5
75	1294.5
76	883.0
77	629.5
78	376.0
79	391.0
80	406.0
81	268.5
82	131.0
83	97.5
84	64.0
85	58.0
86	52.0
87	32.5
88	13.0
89	9.5
90	6.0
91	5.5
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1782252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.626463515003397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74333005817824	19.145432812125936
2	7.661012411021987	3.7732728525604227
3	2.8354152855910217	2.0947875324147067
4	1.5825868261911817	1.5589406693808863
5	0.9988024455189191	1.2298485991633912
6	0.6928310469144802	1.0237187099340632
7	0.5810057002660053	1.0015680975726846
8	0.47398292467357406	0.9338018560966699
9	0.3946884698614539	0.874780308255205
>10	5.204832530968208	30.300956180945978
>50	1.4484449646446629	25.176786727097166
>100	0.3802763174799409	11.89766898817914
>500	0.002093264017749983	0.35471914436076823
>1k	4.651697817222184E-4	0.1756126104650598
>5k	2.325848908611092E-4	0.4581049114479936
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7998	0.4487580880818201	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024968410752239302	0.0
2	0.0	0.0	0.0	0.08371431200526076	0.0
3	0.0	0.0	0.0	0.13449276533284854	0.0
4	0.0	0.0	0.0	0.22982159649701614	0.0
5	0.0	0.0	0.0	0.4179543633560237	0.0
6	0.0	0.0	0.0	0.7137037859965931	0.0
7	0.0	0.0	0.0	0.8639070120274799	0.0
8	0.0	0.0	0.0	1.2279408299163082	0.0
9	0.0	0.0	0.0	1.372140415609016	0.0
10	0.0	0.0	0.0	1.6066751503154435	0.0
11	0.0	0.0	0.0	1.8999557862748926	0.0
12	0.0	0.0	0.0	2.1205194327177077	0.0
13	0.0	0.0	0.0	2.235430230966216	0.0
14	0.0	0.0	0.0	2.2966449189003577	0.0
15	0.0	0.0	0.0	2.3705963017575518	0.0
16	0.0	0.0	0.0	2.5223705738582423	0.0
17	0.0	0.0	0.0	2.711344972540359	0.0
18	0.0	0.0	0.0	2.9249511292454713	0.0
19	0.0	0.0	0.0	3.0571434342618216	0.0
20	0.0	0.0	0.0	3.1824343583286763	0.0
21	0.0	0.0	0.0	3.358110974205668	0.0
22	0.0	0.0	0.0	3.5625152896447867	0.0
23	0.0	0.0	0.0	3.7932907355413263	0.0
24	0.0	0.0	0.0	3.9750832093329116	5.610878820727933E-5
25	0.0	0.0	0.0	4.13185116358405	5.610878820727933E-5
26	0.0	0.0	0.0	4.279585602933817	5.610878820727933E-5
27	0.0	0.0	0.0	4.431640418975543	5.610878820727933E-5
28	0.0	0.0	0.0	4.601145068149735	5.610878820727933E-5
29	0.0	0.0	0.0	4.786809048327622	5.610878820727933E-5
30	0.0	0.0	0.0	5.001705707161501	5.610878820727933E-5
31	0.0	0.0	0.0	5.206053913812412	5.610878820727933E-5
32	0.0	0.0	0.0	5.40759668105296	5.610878820727933E-5
33	0.0	0.0	0.0	5.618790159865159	5.610878820727933E-5
34	0.0	0.0	0.0	5.837502216297135	5.610878820727933E-5
35	5.610878820727933E-5	0.0	0.0	6.069175402804991	5.610878820727933E-5
36	5.610878820727933E-5	0.0	0.0	6.300287501430774	5.610878820727933E-5
37	5.610878820727933E-5	0.0	0.0	6.538244872217846	5.610878820727933E-5
38	5.610878820727933E-5	0.0	0.0	6.779624879085562	5.610878820727933E-5
39	5.610878820727933E-5	0.0	0.0	7.050518108550306	5.610878820727933E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	35	1.2124292E-7	45.000004	1
ACGTGCG	30	2.166178E-6	45.000004	1
TATTACG	35	1.2124292E-7	45.000004	1
TTCGGTC	20	7.034333E-4	45.0	33
TCGCGAT	20	7.034333E-4	45.0	40
CGACGGT	140	0.0	41.785717	28
CGTTTTT	5105	0.0	41.606266	1
TATACGG	130	0.0	41.53846	2
TACGACG	45	1.929402E-8	40.0	1
TAGGGCG	685	0.0	39.744526	5
ACGGGAC	555	0.0	39.72973	5
GTACGTC	40	3.459645E-7	39.375	15
AGGGCGA	1530	0.0	38.97059	6
TAAGGGA	2525	0.0	38.67327	4
CGGGATC	355	0.0	38.661972	6
GCCGATC	35	6.250224E-6	38.57143	9
GGGCGAT	2920	0.0	38.527397	7
TGGGCGA	880	0.0	38.352272	6
ACGGGTC	190	0.0	37.894737	5
ATCGCCA	95	0.0	37.894737	24
>>END_MODULE