FastQCFastQC Report
Thu 26 May 2016
SRR1547986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547986_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2400072
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.2540757110620015No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29310.12212133635990921No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG207.034823E-445.01
TACGTTA351.2126657E-745.033
CGGTCTA3450.044.34782831
TACGGGA8700.041.896554
TAATGCG1250.041.3999981
TACGCCT501.0822987E-940.524
CTCACGA3900.040.38461724
TAACGCC1400.040.1785712
CGCGTAC451.9297659E-840.032
CGACGGT4000.039.937528
CTACGGG4650.039.677423
ATCGCCT403.460318E-739.37514
AGGGCGA20950.039.3078736
ACGGGAT11050.039.2986455
ACGTAGG2750.039.2727282
TTGCGAA356.2511335E-638.57142627
AATACGG2400.038.4375042
GTTAGCG1350.038.3333321
TAAGGGA26600.037.9793244
GGGCGAT36200.037.976527
Produced by FastQC (version 0.11.5)