FastQCFastQC Report
Thu 26 May 2016
SRR1547985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547985_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2312863
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55030.2379302189537383No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30460.13169824585373194No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA29110.1258613242548305No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24470.10579960853712476No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG253.8920363E-545.09
TATACGG1350.043.3333322
CGACGGT3000.041.2528
ACGCTAG900.040.01
TTACGAG900.040.01
CGTAAGG3400.039.7058832
TCACGAC3350.038.95522325
TAAGGGA29050.038.881244
ACGGGAT7900.038.7341775
CCACGTA356.2510335E-638.57142633
TAGGGAT30600.038.3088265
CACGACG3350.038.28358526
TACGGGA7650.037.9411774
TAGTAAG3500.037.928571
TAGGGAC20800.037.860585
TTAGGGA27600.037.7445684
TGTTACG601.5643309E-1037.5000041
ACGCTAA301.1402791E-437.5000047
ATTGGGA23650.037.293874
GCGTAAG1750.037.2857131