##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547985_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2312863
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.825426322268115	31.0	31.0	31.0	30.0	33.0
2	31.171375909424814	31.0	31.0	33.0	30.0	34.0
3	31.38166376477984	31.0	31.0	34.0	30.0	34.0
4	35.24843538073807	37.0	35.0	37.0	33.0	37.0
5	35.05995167028916	35.0	35.0	37.0	32.0	37.0
6	35.0863112082298	37.0	35.0	37.0	32.0	37.0
7	35.46653390192156	35.0	35.0	37.0	35.0	37.0
8	35.53364552937204	37.0	35.0	37.0	35.0	37.0
9	37.337033365140954	39.0	37.0	39.0	35.0	39.0
10	36.455611508334044	38.0	35.0	39.0	32.0	39.0
11	36.08910601276427	38.0	35.0	39.0	31.0	39.0
12	35.5755377642342	37.0	35.0	39.0	30.0	39.0
13	35.31929042057398	37.0	35.0	39.0	30.0	39.0
14	36.25651930097027	38.0	35.0	40.0	30.0	41.0
15	36.452339373322154	38.0	35.0	40.0	30.0	41.0
16	36.5958580339605	38.0	35.0	40.0	31.0	41.0
17	36.544074594993305	38.0	35.0	40.0	31.0	41.0
18	36.44605019839048	38.0	35.0	40.0	30.0	41.0
19	36.41752364926068	38.0	35.0	40.0	30.0	41.0
20	36.293469608878695	38.0	34.0	40.0	30.0	41.0
21	36.15580602915088	38.0	34.0	40.0	30.0	41.0
22	36.041064689088806	38.0	34.0	40.0	30.0	41.0
23	35.94847727686422	38.0	34.0	40.0	29.0	41.0
24	35.81181808001598	38.0	34.0	40.0	29.0	41.0
25	35.62666098251388	38.0	34.0	40.0	29.0	41.0
26	35.38904984860755	37.0	34.0	40.0	27.0	41.0
27	35.45618352665073	38.0	34.0	40.0	27.0	41.0
28	35.47563604069934	38.0	34.0	40.0	28.0	41.0
29	35.45172195672636	38.0	34.0	40.0	28.0	41.0
30	35.370972253868906	37.0	34.0	40.0	27.0	41.0
31	35.27757156390154	37.0	34.0	40.0	27.0	41.0
32	35.152763479721884	37.0	34.0	40.0	27.0	41.0
33	35.04954033161497	37.0	33.0	40.0	26.0	41.0
34	34.900171778440836	37.0	33.0	40.0	25.0	41.0
35	34.784976023223166	37.0	33.0	40.0	25.0	41.0
36	34.67705999015074	37.0	33.0	40.0	24.0	41.0
37	34.63184762781021	37.0	33.0	40.0	24.0	41.0
38	34.50990179703683	37.0	33.0	40.0	24.0	41.0
39	34.46262619100224	37.0	33.0	40.0	24.0	41.0
40	34.206816400279656	37.0	33.0	40.0	23.0	41.0
41	34.1181168102045	36.0	33.0	40.0	23.0	41.0
42	34.076876581103164	36.0	33.0	40.0	23.0	41.0
43	34.02087542582505	36.0	33.0	40.0	23.0	41.0
44	33.83044521011404	36.0	32.0	40.0	23.0	41.0
45	33.686836185282054	36.0	32.0	40.0	23.0	41.0
46	33.592984539075594	35.0	32.0	40.0	23.0	41.0
47	33.517745754936634	35.0	32.0	40.0	23.0	41.0
48	33.41153107641914	35.0	32.0	39.0	22.0	41.0
49	33.284029361012735	35.0	32.0	39.0	22.0	41.0
50	33.14917528621453	35.0	31.0	39.0	21.0	41.0
51	32.81812454953017	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	5.0
12	4.0
13	7.0
14	34.0
15	128.0
16	417.0
17	876.0
18	1833.0
19	3356.0
20	5484.0
21	8712.0
22	12434.0
23	17674.0
24	24573.0
25	32714.0
26	41239.0
27	48522.0
28	57386.0
29	67290.0
30	80328.0
31	95906.0
32	114637.0
33	142268.0
34	203135.0
35	229650.0
36	199942.0
37	246082.0
38	319055.0
39	359138.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.53911321163424	24.301266439041136	28.48019964866055	11.67942070066407
2	30.60652533245592	26.75545417086961	28.509384256655064	14.128636240019404
3	30.829841629184262	26.278512821554926	28.907548782612718	13.984096766648088
4	26.519599301817703	28.74450410595007	27.77600748509531	16.959889107136913
5	23.368785786274415	33.49454766667978	28.155191206742465	14.981475340303337
6	21.67482466536064	43.10769812133274	24.924519956434946	10.292957256871677
7	90.30405173155522	3.3295530258385386	4.69344703944851	1.6729482031577314
8	90.53099989061177	2.6620253772056537	4.7437310381116395	2.0632436940709415
9	85.64990663087264	5.284229978169913	6.397568727590004	2.668294663367437
10	42.235748507369436	33.219996169249974	12.384996430830533	12.159258892550055
11	34.34245781094686	26.429105398806584	22.438596665690962	16.78984012455558
12	32.51048592156129	22.85530963139624	26.029557306247714	18.604647140794764
13	25.13931867127452	29.101853417171704	27.26573082798246	18.493097083571314
14	21.01680903711115	31.570871253507015	28.153764403684956	19.258555305696877
15	19.41450920352827	26.617529875310385	34.78359937445495	19.1843615467064
16	23.222646564020437	24.070556708287523	33.39791418687575	19.308882540816295
17	23.02981196897525	23.65929153607455	26.784509069495254	26.526387425454946
18	23.975350031540994	25.83698212994025	29.638288130338893	20.549379708179863
19	26.567894423491577	27.82983687317407	25.530176236119477	20.072092467214876
20	28.930637050270597	26.396159219115013	25.286927933042296	19.3862757975721
21	26.648746596750435	26.840284098106977	27.358516263176853	19.152453041965735
22	26.33506610637984	23.431954248911413	26.74122937675081	23.491750267957936
23	23.015846593594173	29.04988319671334	25.91260269198824	22.02166751770425
24	22.271617471506094	27.160363584008216	30.643449266126012	19.924569678359678
25	23.02583421499674	27.74249923147199	27.655550717876505	21.57611583565477
26	22.490956014255925	29.29274237168393	25.430689150200426	22.785612463859728
27	21.69825882466882	28.705721004659594	27.80437060042035	21.79164957025124
28	19.80099988628812	27.80769980755453	30.722009907201592	21.669290398955752
29	20.794443942421147	27.21873279999723	29.813914615781396	22.172908641800227
30	21.73678250722157	27.488485050779055	29.4129829566213	21.361749485378077
31	26.588820868335045	26.34613463918961	25.31823112739492	21.746813365080424
32	25.806457191800813	27.108825728112734	26.277258964322574	20.807458115763882
33	25.184673713920798	26.716800778947995	25.430645913744133	22.66787959338707
34	21.239044422432286	28.007971073081283	27.56899133238761	23.183993172098823
35	21.989240175488128	27.53630457143376	28.383479695943947	22.090975557134165
36	26.52379323807765	27.472790217146454	25.56995377590458	20.43346276887131
37	22.225354463277764	29.142798341276592	26.078068610203026	22.553778585242622
38	22.438207537584372	30.313944232753947	24.95871999335888	22.289128236302798
39	22.013366118096922	27.98298040134673	25.754962572361613	24.248690908194735
40	25.096557816005532	25.404963458708966	27.227942165186608	22.27053656009889
41	20.70049112290698	24.778034842530662	28.517599183349812	26.003874851212544
42	22.53544632777644	25.641293928780044	26.385177159217815	25.438082584225697
43	22.330548761426854	25.31390748176611	27.06667018323178	25.28887357357526
44	22.027417966390573	26.710185601135912	28.414566708015133	22.84782972445839
45	22.311395011291204	28.456549307070933	25.232493234575504	23.999562447062363
46	22.765507511685733	27.174847796864753	27.12305052223154	22.936594169217976
47	22.57669390707534	27.041549802128355	27.975284312127435	22.40647197866886
48	22.618503560305996	26.824805446755818	28.545356988286812	22.011334004651378
49	23.17015750608661	25.5662354406638	28.173480227752357	23.090126825497233
50	21.40239175428895	27.62640934633828	28.544794914355066	22.426403985017703
51	20.879749470677684	27.696279459699944	27.323840625233746	24.100130444388622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1133.0
1	1984.5
2	2836.0
3	6688.0
4	10540.0
5	8386.0
6	6232.0
7	6174.5
8	6117.0
9	6562.0
10	7007.0
11	7242.0
12	7477.0
13	7872.0
14	8267.0
15	8226.0
16	8185.0
17	8445.0
18	8705.0
19	9168.0
20	9631.0
21	10285.0
22	10939.0
23	11694.5
24	12450.0
25	14383.5
26	19942.5
27	23568.0
28	26870.0
29	30172.0
30	35912.5
31	41653.0
32	48935.0
33	56217.0
34	60235.5
35	64254.0
36	70710.0
37	77166.0
38	82518.5
39	87871.0
40	101738.0
41	115605.0
42	134113.5
43	152622.0
44	155919.0
45	159216.0
46	163351.0
47	167486.0
48	174862.5
49	182239.0
50	176465.0
51	170691.0
52	160673.5
53	150656.0
54	141827.5
55	132999.0
56	128046.5
57	123094.0
58	114587.0
59	106080.0
60	97822.0
61	89564.0
62	84385.5
63	79207.0
64	70949.0
65	62691.0
66	53759.5
67	44828.0
68	36725.5
69	28623.0
70	24044.0
71	19465.0
72	16365.0
73	13265.0
74	10919.5
75	6532.5
76	4491.0
77	3468.0
78	2445.0
79	1853.0
80	1261.0
81	938.0
82	615.0
83	430.5
84	246.0
85	168.5
86	91.0
87	66.0
88	41.0
89	31.5
90	22.0
91	14.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2312863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.376905208222944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67358314794308	16.235010514051055
2	7.065272735937494	2.8793678562088063
3	2.365189447213239	1.4458572349606018
4	1.1802539338596532	0.9619969012756154
5	0.7606740075664242	0.7750091073270046
6	0.5744797638957522	0.7023671815767624
7	0.3976911286159018	0.5672600100970202
8	0.3286485116070424	0.5357471654272209
9	0.2805765845856028	0.5145554220972998
>10	4.532228223127523	23.64697605716771
>50	2.1300084498768155	31.069501463805988
>100	0.7027052848882336	18.65257910356
>500	0.006082146618226664	0.83381916224061
>1k	0.002389414742874761	0.9363756437626131
>5k	2.1721952207952376E-4	0.24357717644177074
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5503	0.2379302189537383	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3046	0.13169824585373194	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2911	0.1258613242548305	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2447	0.10579960853712476	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026374238335776914	0.0
2	0.0	0.0	0.0	0.10683728348804059	0.0
3	0.0	0.0	0.0	0.16153140069256156	0.0
4	0.0	0.0	0.0	0.32414371279232707	0.0
5	0.0	0.0	0.0	0.5733586468372749	0.0
6	0.0	0.0	0.0	0.9445436240711188	0.0
7	0.0	0.0	0.0	1.1024863988917633	0.0
8	0.0	0.0	0.0	1.5024668560135208	0.0
9	0.0	0.0	0.0	1.6397426047284254	0.0
10	0.0	0.0	0.0	1.898469559156768	0.0
11	0.0	0.0	0.0	2.2412914210655797	0.0
12	0.0	0.0	0.0	2.5148917164570492	0.0
13	0.0	0.0	0.0	2.656274928519329	0.0
14	0.0	0.0	0.0	2.714903563246072	0.0
15	0.0	0.0	0.0	2.8075592890715964	0.0
16	0.0	0.0	0.0	2.9632537681652567	0.0
17	0.0	0.0	0.0	3.133129804921433	0.0
18	0.0	0.0	0.0	3.340275666998002	0.0
19	0.0	0.0	0.0	3.464061641351001	0.0
20	0.0	0.0	0.0	3.5938142466717657	0.0
21	0.0	0.0	0.0	3.7706513528903356	0.0
22	0.0	0.0	0.0	3.9562222232791133	0.0
23	0.0	0.0	0.0	4.156450252349577	0.0
24	0.0	0.0	0.0	4.316338667703189	0.0
25	0.0	0.0	0.0	4.4687904125752365	0.0
26	0.0	0.0	0.0	4.602218116680495	0.0
27	0.0	0.0	0.0	4.736726732192958	0.0
28	0.0	0.0	0.0	4.878628781730695	0.0
29	0.0	0.0	0.0	5.039381926210069	0.0
30	0.0	0.0	0.0	5.2373184230972605	0.0
31	0.0	0.0	0.0	5.406070311989945	0.0
32	0.0	0.0	0.0	5.578454063210835	0.0
33	0.0	0.0	0.0	5.749583957199367	0.0
34	0.0	0.0	0.0	5.939435236760673	0.0
35	0.0	0.0	0.0	6.156698429608671	0.0
36	0.0	0.0	0.0	6.341880171890856	0.0
37	0.0	0.0	0.0	6.531169377520415	0.0
38	0.0	0.0	0.0	6.751761777502602	0.0
39	0.0	0.0	0.0	7.090562648976615	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	25	3.8920363E-5	45.0	9
TATACGG	135	0.0	43.333332	2
CGACGGT	300	0.0	41.25	28
ACGCTAG	90	0.0	40.0	1
TTACGAG	90	0.0	40.0	1
CGTAAGG	340	0.0	39.705883	2
TCACGAC	335	0.0	38.955223	25
TAAGGGA	2905	0.0	38.88124	4
ACGGGAT	790	0.0	38.734177	5
CCACGTA	35	6.2510335E-6	38.571426	33
TAGGGAT	3060	0.0	38.308826	5
CACGACG	335	0.0	38.283585	26
TACGGGA	765	0.0	37.941177	4
TAGTAAG	350	0.0	37.92857	1
TAGGGAC	2080	0.0	37.86058	5
TTAGGGA	2760	0.0	37.744568	4
TGTTACG	60	1.5643309E-10	37.500004	1
ACGCTAA	30	1.1402791E-4	37.500004	7
ATTGGGA	2365	0.0	37.29387	4
GCGTAAG	175	0.0	37.285713	1
>>END_MODULE