FastQCFastQC Report
Thu 26 May 2016
SRR1547984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547984_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1352087
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.2456942489647486No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23730.17550645779450583No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATT253.8911712E-545.00000429
GTATACG502.1827873E-1145.0000041
TATAACG253.8911712E-545.00000428
ACCGGAT207.033729E-445.045
CGTATTA207.033729E-445.038
CACGATA207.033729E-445.039
TACGGGT551.8189894E-1245.04
CACGGGT302.165787E-644.9999964
TCTAGCG302.165787E-644.9999961
TACGGGA5300.042.8773574
CGCATCG850.042.3529421
TCACGAC2100.041.78571725
CGGTCTA2050.041.7073231
CGTTAGG1300.041.538462
CGACGGT2100.040.71428728
ACGGTCT2100.040.71428730
CGTAAGG2550.040.5882342
TTACGGG3900.040.3846133
AGGGCGA14750.040.118646
ATCGTCC451.9286745E-840.00000415