##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547984_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1352087
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.847245036746894	31.0	31.0	31.0	30.0	33.0
2	31.18470335118968	31.0	31.0	33.0	30.0	34.0
3	31.38939210272712	31.0	31.0	34.0	30.0	34.0
4	35.261457287881626	37.0	35.0	37.0	33.0	37.0
5	35.06652012777284	35.0	35.0	37.0	32.0	37.0
6	35.09758173845322	37.0	35.0	37.0	32.0	37.0
7	35.47763346589384	35.0	35.0	37.0	35.0	37.0
8	35.54425787689698	37.0	35.0	37.0	35.0	37.0
9	37.350263703445115	39.0	37.0	39.0	35.0	39.0
10	36.528420138644925	38.0	35.0	39.0	32.0	39.0
11	36.12102327734828	38.0	35.0	39.0	31.0	39.0
12	35.31545011526625	37.0	35.0	39.0	30.0	39.0
13	34.844157217693834	37.0	33.0	39.0	27.0	39.0
14	35.73056615439687	38.0	34.0	40.0	27.0	41.0
15	36.09927541644879	38.0	34.0	40.0	30.0	41.0
16	36.30793654550336	38.0	34.0	40.0	31.0	41.0
17	36.25337126974817	38.0	34.0	40.0	30.0	41.0
18	36.18986795968011	38.0	35.0	40.0	30.0	41.0
19	36.11944497654367	38.0	34.0	40.0	30.0	41.0
20	35.96589716490137	37.0	34.0	40.0	30.0	41.0
21	35.84523555067093	37.0	34.0	40.0	30.0	41.0
22	35.709136320369915	37.0	34.0	40.0	29.0	41.0
23	35.68544775595061	37.0	34.0	40.0	29.0	41.0
24	35.555153625469366	37.0	34.0	40.0	29.0	41.0
25	35.35576039115826	37.0	34.0	40.0	29.0	41.0
26	35.113351433746494	36.0	34.0	40.0	27.0	41.0
27	35.13948658629216	36.0	34.0	40.0	27.0	41.0
28	35.197456968375555	36.0	34.0	40.0	27.0	41.0
29	35.22218022952665	36.0	34.0	40.0	28.0	41.0
30	35.14136072604796	36.0	34.0	40.0	27.0	41.0
31	34.9801403312065	36.0	33.0	40.0	27.0	41.0
32	34.84131864295715	36.0	33.0	40.0	27.0	41.0
33	34.71689469686492	36.0	33.0	40.0	26.0	41.0
34	34.56709220634471	36.0	33.0	40.0	25.0	41.0
35	34.426828303208296	36.0	33.0	40.0	24.0	41.0
36	34.271929247156436	36.0	33.0	40.0	24.0	41.0
37	34.14989789858197	36.0	33.0	40.0	23.0	41.0
38	34.0835937332435	36.0	33.0	40.0	23.0	41.0
39	34.02434754568308	36.0	33.0	40.0	23.0	41.0
40	33.792062197181096	36.0	33.0	40.0	23.0	41.0
41	33.71644576125649	35.0	32.0	40.0	23.0	41.0
42	33.66837858806423	35.0	32.0	40.0	23.0	41.0
43	33.593732503899524	35.0	32.0	40.0	23.0	41.0
44	33.42712192336736	35.0	32.0	40.0	22.0	41.0
45	33.28149372044846	35.0	32.0	39.0	22.0	41.0
46	33.24881165191293	35.0	32.0	39.0	22.0	41.0
47	33.189095080420124	35.0	32.0	39.0	22.0	41.0
48	33.095278632218196	35.0	32.0	39.0	22.0	41.0
49	32.98752003384398	35.0	31.0	39.0	21.0	41.0
50	32.83555496059056	35.0	31.0	39.0	20.0	41.0
51	32.48678598344633	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	10.0
14	35.0
15	71.0
16	214.0
17	547.0
18	1207.0
19	2074.0
20	3554.0
21	5319.0
22	8002.0
23	11401.0
24	15629.0
25	20573.0
26	26045.0
27	30273.0
28	35036.0
29	41413.0
30	48783.0
31	58306.0
32	70259.0
33	88703.0
34	134549.0
35	154697.0
36	108119.0
37	130801.0
38	168816.0
39	187626.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.81528925283654	24.47394287497772	28.314967897775812	12.39579997440993
2	30.329483235916033	26.634306816055474	29.150934814105895	13.885275133922596
3	30.104571673272506	26.225827184197463	29.634261700615415	14.03533944191461
4	27.149658269031505	28.67101007553508	29.217942336550827	14.961389318882587
5	23.353009088912177	34.238921016177216	28.336933939901797	14.071135955008812
6	22.480727941323302	43.4446895798865	23.932631554034614	10.141950924755582
7	90.43478711059274	3.6414816502192533	4.5537750159568136	1.3699562232311973
8	90.4611167772488	3.2188757084418387	4.483883063737762	1.836124450571598
9	85.45692695810256	6.085185346800909	5.897549492007541	2.5603382030890023
10	46.51165198689138	32.23520379975549	10.972296901013026	10.280847312340107
11	41.73533211990057	22.1688397270294	21.42902046983663	14.666807683233401
12	38.25530457729421	21.38893429195015	24.33696944057594	16.0187916901797
13	25.27832898326809	33.26590670570755	24.782428941332917	16.673335369691447
14	19.20786162428897	36.344480791546694	27.144924845812433	17.302732738351896
15	17.634959880540233	26.11917724229284	39.73043154767408	16.51543132949285
16	22.04473528700446	21.834689631658318	37.735885338739294	18.384689742597924
17	21.44728852507272	22.45099612672853	26.542818620399427	29.558896727799322
18	23.55085138752166	24.684358329012852	31.64478321291455	20.120007070550933
19	28.693567795563453	25.685847138534722	25.018286545170543	20.602298520731285
20	29.624055256799302	26.5402300295765	24.45493522236365	19.380779491260547
21	25.91149829855623	27.20032068942309	27.29728190567619	19.59089910634449
22	25.37403288397862	23.314993783684038	26.24660987051869	25.064363461818655
23	21.545359137392786	28.926910768315945	26.014820052259953	23.51291004203132
24	22.093992472377888	26.433062369507287	32.84892170400278	18.624023454112052
25	21.92529031046079	25.70300579770385	31.330898085700102	21.040805806135257
26	20.411778236163798	31.869546856082486	25.08255755731695	22.63611735043677
27	20.67736765459619	31.365363323513947	28.166012985850763	19.7912560360391
28	18.260585302572984	28.644754368616816	33.16125367672347	19.933406652086738
29	19.632908237413716	27.039236380499183	32.077077880343495	21.2507775017436
30	22.14975811467753	28.816932638210414	29.323926640815273	19.709382606296785
31	28.228287085076627	26.69229125048906	25.178853135929863	19.900568528504454
32	29.385239263449765	26.52299741066958	25.046613124747154	19.045150201133506
33	27.50910259472948	26.46893284233929	24.943883048945814	21.078081513985417
34	21.500243697336042	28.330573402451176	27.031766446981592	23.13741645323119
35	22.001320920917074	26.934065633350517	28.51184871979392	22.55276472593849
36	29.35018234773354	25.449545776270316	26.08759643425312	19.112675441743026
37	22.532203918830668	30.24738792696032	27.14766135611096	20.072746798098052
38	22.400703505025934	30.98151228434265	24.07204565978373	22.545738550847688
39	21.640471360200934	29.782033256735694	26.576322381621893	22.001173001441476
40	24.75543363703667	26.135966102772972	25.68769613197967	23.420904128210683
41	20.363038768954954	24.0944554603365	28.306536487666843	27.2359692830417
42	22.567334794284687	25.974955753586865	26.187146241329145	25.270563210799306
43	22.284734636158767	25.6257918314428	27.017640137062187	25.071833395336245
44	21.950954339476674	27.151063504049667	27.91980101872143	22.97818113775223
45	20.185535398239907	31.888406589220956	24.71889752656449	23.207160485974647
46	24.076705123264997	29.425473360811843	25.904176284514236	20.593645231408924
47	22.27386255470247	27.774544093686277	26.796056762619564	23.155536588991684
48	22.748905950578624	26.31857269539608	29.65252975585151	21.279991598173787
49	23.217514849266358	23.881229536265046	30.219061347383715	22.682194267084885
50	22.200198655855726	28.85346874868259	27.469460175269788	21.476872420191896
51	20.756134775350997	30.44811465534392	24.928425463746045	23.867325105559036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	812.0
1	1251.0
2	1690.0
3	4106.0
4	6522.0
5	5207.0
6	3892.0
7	4054.5
8	4217.0
9	4616.5
10	5016.0
11	5325.0
12	5634.0
13	5816.0
14	5998.0
15	6029.0
16	6060.0
17	5922.0
18	5784.0
19	5859.5
20	5935.0
21	6130.5
22	6326.0
23	6482.0
24	6638.0
25	7459.5
26	10093.0
27	11905.0
28	13914.0
29	15923.0
30	18755.5
31	21588.0
32	23970.0
33	26352.0
34	29207.5
35	32063.0
36	35425.0
37	38787.0
38	42846.5
39	46906.0
40	62451.5
41	77997.0
42	92838.0
43	107679.0
44	112719.5
45	117760.0
46	115388.5
47	113017.0
48	108117.5
49	103218.0
50	98149.5
51	93081.0
52	88575.0
53	84069.0
54	76708.0
55	69347.0
56	68122.5
57	66898.0
58	64202.0
59	61506.0
60	56790.0
61	52074.0
62	46874.5
63	41675.0
64	36327.5
65	30980.0
66	26976.0
67	22972.0
68	19009.5
69	15047.0
70	13247.0
71	11447.0
72	9350.5
73	7254.0
74	5940.0
75	3515.5
76	2405.0
77	1889.5
78	1374.0
79	1080.0
80	786.0
81	540.5
82	295.0
83	216.0
84	137.0
85	106.5
86	76.0
87	51.0
88	26.0
89	17.5
90	9.0
91	4.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1352087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.06627304095235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.48288622714503	16.35057843198748
2	6.332196965403253	2.541271865137431
3	1.9517408848926827	1.1749249650433957
4	1.0256423498128162	0.8232327773483175
5	0.6169898569297655	0.6190343466325401
6	0.4509457472150685	0.5429280294164307
7	0.3640050919025715	0.5112957893689768
8	0.29249581568191513	0.46954407206475046
9	0.2602598213202044	0.4700200172580624
>10	4.084812200574418	21.623970814644707
>50	2.3791219142739406	34.28397803432408
>100	0.7544014750716661	19.599189441031108
>500	0.003376237332493782	0.4770999933833216
>1k	0.0011254124441645939	0.5129314223594121
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3322	0.2456942489647486	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2373	0.17550645779450583	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.395973779793756E-5	0.0	0.0	0.02152228369919983	0.0
2	7.395973779793756E-5	0.0	0.0	0.09659141756410645	0.0
3	7.395973779793756E-5	0.0	0.0	0.14429544844377618	0.0
4	7.395973779793756E-5	0.0	0.0	0.28733358134498743	0.0
5	7.395973779793756E-5	0.0	0.0	0.48199561122915907	0.0
6	7.395973779793756E-5	0.0	0.0	0.7390796598147901	0.0
7	7.395973779793756E-5	0.0	0.0	0.8379638292506325	0.0
8	7.395973779793756E-5	0.0	0.0	1.1106533825116283	0.0
9	7.395973779793756E-5	0.0	0.0	1.1889767448396442	0.0
10	7.395973779793756E-5	0.0	0.0	1.358714343085911	0.0
11	7.395973779793756E-5	0.0	0.0	1.6081065789405564	0.0
12	7.395973779793756E-5	0.0	0.0	1.799218541410427	0.0
13	7.395973779793756E-5	0.0	0.0	1.8822753269575108	0.0
14	7.395973779793756E-5	0.0	0.0	1.917554121887127	0.0
15	7.395973779793756E-5	0.0	0.0	1.9746510394671348	0.0
16	7.395973779793756E-5	0.0	0.0	2.1043024598269193	0.0
17	7.395973779793756E-5	0.0	0.0	2.245639518758778	0.0
18	7.395973779793756E-5	0.0	0.0	2.4287638295464715	0.0
19	7.395973779793756E-5	0.0	0.0	2.520695783629308	0.0
20	7.395973779793756E-5	0.0	0.0	2.617509080406808	0.0
21	7.395973779793756E-5	0.0	0.0	2.747900098144572	0.0
22	7.395973779793756E-5	0.0	0.0	2.8882756804850573	0.0
23	7.395973779793756E-5	0.0	0.0	3.0352336794895596	0.0
24	7.395973779793756E-5	0.0	0.0	3.146099326448668	0.0
25	7.395973779793756E-5	0.0	0.0	3.241803227159199	0.0
26	7.395973779793756E-5	0.0	0.0	3.335584174686984	0.0
27	7.395973779793756E-5	0.0	0.0	3.4440091502987604	0.0
28	7.395973779793756E-5	0.0	0.0	3.546443387148904	0.0
29	7.395973779793756E-5	0.0	0.0	3.6699561492714596	0.0
30	7.395973779793756E-5	0.0	0.0	3.802344079929768	0.0
31	7.395973779793756E-5	0.0	0.0	3.9341403326856925	0.0
32	7.395973779793756E-5	0.0	0.0	4.0444143017424175	0.0
33	7.395973779793756E-5	0.0	0.0	4.172660487084041	0.0
34	7.395973779793756E-5	0.0	0.0	4.318952848448362	0.0
35	7.395973779793756E-5	0.0	0.0	4.483143466359783	0.0
36	7.395973779793756E-5	0.0	0.0	4.6289181095595175	0.0
37	7.395973779793756E-5	0.0	0.0	4.763524832351765	0.0
38	7.395973779793756E-5	0.0	0.0	4.93259679295785	0.0
39	7.395973779793756E-5	0.0	0.0	5.217489702955505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATT	25	3.8911712E-5	45.000004	29
GTATACG	50	2.1827873E-11	45.000004	1
TATAACG	25	3.8911712E-5	45.000004	28
ACCGGAT	20	7.033729E-4	45.0	45
CGTATTA	20	7.033729E-4	45.0	38
CACGATA	20	7.033729E-4	45.0	39
TACGGGT	55	1.8189894E-12	45.0	4
CACGGGT	30	2.165787E-6	44.999996	4
TCTAGCG	30	2.165787E-6	44.999996	1
TACGGGA	530	0.0	42.877357	4
CGCATCG	85	0.0	42.35294	21
TCACGAC	210	0.0	41.785717	25
CGGTCTA	205	0.0	41.70732	31
CGTTAGG	130	0.0	41.53846	2
CGACGGT	210	0.0	40.714287	28
ACGGTCT	210	0.0	40.714287	30
CGTAAGG	255	0.0	40.588234	2
TTACGGG	390	0.0	40.384613	3
AGGGCGA	1475	0.0	40.11864	6
ATCGTCC	45	1.9286745E-8	40.000004	15
>>END_MODULE