FastQCFastQC Report
Thu 26 May 2016
SRR1547983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547983_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1903285
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44940.23611808005632368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG950.045.0000041
GGTACGC207.034456E-445.08
CGTAACA207.034456E-445.035
CCGGTTA351.2124838E-745.031
GCGAATA302.1662563E-644.9999961
CGTACGA302.1662563E-644.99999636
TCATACG302.1662563E-644.9999961
GCGTAAT302.1662563E-644.99999632
TCGAACG850.042.352941
TATCGCG700.041.7857131
CGGTCTA2450.041.3265331
TACGTAG1350.040.0000041
TACGCGG900.040.02
CGTAAGG4400.039.8863642
AGGGCGA18050.039.390586
TACGGGA7850.039.2675174
GGCGATA7450.038.9597328
CTACGGG4800.038.9062463
ACGGGAT8550.038.684215
GGGCGAT32050.038.6817477
Produced by FastQC (version 0.11.5)