##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547983_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1903285
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83508775616894	31.0	31.0	31.0	30.0	33.0
2	31.184020259708873	31.0	31.0	33.0	30.0	34.0
3	31.384690154128258	31.0	31.0	34.0	30.0	34.0
4	35.25282288254255	37.0	35.0	37.0	33.0	37.0
5	35.05381537709802	35.0	35.0	37.0	32.0	37.0
6	35.08054074928348	37.0	35.0	37.0	32.0	37.0
7	35.47668636068692	35.0	35.0	37.0	35.0	37.0
8	35.552576203774	37.0	35.0	37.0	35.0	37.0
9	37.367375879072235	39.0	37.0	39.0	35.0	39.0
10	36.47181215635073	38.0	35.0	39.0	32.0	39.0
11	36.07754435095112	38.0	35.0	39.0	31.0	39.0
12	35.328985937471266	37.0	35.0	39.0	30.0	39.0
13	34.95103255686878	37.0	34.0	39.0	27.0	39.0
14	35.85876366387588	38.0	34.0	40.0	27.0	41.0
15	36.14003315320617	38.0	34.0	40.0	30.0	41.0
16	36.303196315843394	38.0	34.0	40.0	30.0	41.0
17	36.242106148054546	38.0	34.0	40.0	30.0	41.0
18	36.15352824196061	38.0	34.0	40.0	30.0	41.0
19	36.11544356205193	38.0	34.0	40.0	30.0	41.0
20	35.98748794846804	38.0	34.0	40.0	30.0	41.0
21	35.85798868797894	38.0	34.0	40.0	29.0	41.0
22	35.74672579251137	37.0	34.0	40.0	29.0	41.0
23	35.71385945877785	37.0	34.0	40.0	29.0	41.0
24	35.5905789201302	37.0	34.0	40.0	29.0	41.0
25	35.40711401603018	37.0	34.0	40.0	29.0	41.0
26	35.146249248010676	37.0	34.0	40.0	27.0	41.0
27	35.2198593484423	37.0	34.0	40.0	27.0	41.0
28	35.25978873368938	37.0	34.0	40.0	27.0	41.0
29	35.26744024147724	37.0	34.0	40.0	28.0	41.0
30	35.18069653257395	37.0	34.0	40.0	27.0	41.0
31	35.0675232558445	37.0	34.0	40.0	27.0	41.0
32	34.962839511686376	37.0	33.0	40.0	27.0	41.0
33	34.84542724815254	37.0	33.0	40.0	26.0	41.0
34	34.68793743448827	37.0	33.0	40.0	25.0	41.0
35	34.55463002125273	36.0	33.0	40.0	25.0	41.0
36	34.47480172438705	36.0	33.0	40.0	24.0	41.0
37	34.392352170063866	36.0	33.0	40.0	24.0	41.0
38	34.33895291561694	36.0	33.0	40.0	24.0	41.0
39	34.31233682816814	36.0	33.0	40.0	24.0	41.0
40	34.09844400602117	36.0	33.0	40.0	23.0	41.0
41	34.04402335961246	36.0	33.0	40.0	23.0	41.0
42	34.0076667446021	36.0	33.0	40.0	23.0	41.0
43	33.939357479305514	36.0	33.0	40.0	23.0	41.0
44	33.76820234489317	35.0	32.0	40.0	23.0	41.0
45	33.63242656774997	35.0	32.0	40.0	23.0	41.0
46	33.583603086242995	35.0	32.0	40.0	23.0	41.0
47	33.517879876108935	35.0	32.0	39.0	23.0	41.0
48	33.42007949413777	35.0	32.0	39.0	23.0	41.0
49	33.27483955371896	35.0	32.0	39.0	22.0	41.0
50	33.15494736731493	35.0	31.0	39.0	22.0	41.0
51	32.82595459954762	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	12.0
14	30.0
15	116.0
16	282.0
17	699.0
18	1524.0
19	2709.0
20	4684.0
21	7165.0
22	10751.0
23	15084.0
24	20802.0
25	27765.0
26	34421.0
27	41446.0
28	48621.0
29	57117.0
30	67723.0
31	80293.0
32	97893.0
33	123365.0
34	185899.0
35	207256.0
36	154038.0
37	186238.0
38	244615.0
39	282706.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.77791555127057	24.944819089101212	29.19457674494361	12.082688614684612
2	29.952844686949142	26.87810811307818	29.935138457981857	13.233908741990822
3	29.668651830913394	26.528659659483473	29.83142303963936	13.971265469963773
4	26.4770646540061	28.69717357095758	29.16835891629472	15.657402858741598
5	23.530317319791834	33.12893234591772	28.463367283407372	14.877383050883077
6	21.639218509051457	42.82585109429224	25.14804666668418	10.386883729972126
7	91.13285713910423	3.1072067504341176	4.488187528404837	1.2717485820568122
8	91.37512248559727	2.723449194419123	4.1580215259406765	1.7434067940429312
9	86.557767228765	5.316387193720331	5.584660205907156	2.54118537160751
10	45.29043206876532	31.054991764239197	11.932527183264725	11.722048983730762
11	39.98649703013474	23.05860656706694	20.836606183519546	16.118290219278773
12	37.250332976931986	21.35166304573409	24.24938987067097	17.148614106662954
13	25.65175472932325	30.762339849260623	25.642927885208994	17.942977536207135
14	19.772340978886504	34.22487961603228	27.203177663881135	18.799601741200085
15	18.938361832305723	25.267944632569478	38.692418634098416	17.101274901026382
16	22.960618089250953	22.661451122664236	36.463115087861254	17.91481570022356
17	23.588007050967143	22.7039040395947	25.955650362399744	27.75243854703841
18	24.433807863772373	25.259380492149102	30.200101403625833	20.106710240452692
19	27.734206910683373	26.80218674554783	25.275300335998026	20.188306007770777
20	29.90408688136564	26.821206492984494	24.25333042607912	19.021376199570742
21	26.408393908426746	26.62964295940965	28.346831924803695	18.615131207359905
22	25.339820363214127	23.806839227966385	27.15473510273028	23.69860530608921
23	23.65363043369753	27.420118374284463	26.257234202970125	22.669016989047883
24	23.52821569024082	25.191760561345255	31.931056042579016	19.348967705834912
25	22.459379441334324	25.607200182841773	29.681629393390903	22.251790982433004
26	20.833926605842006	30.5947874333061	26.264484824921126	22.306801135930773
27	20.886624966833658	30.41972169170671	28.102359867282097	20.59129347417754
28	19.121308684721416	28.444505158187027	32.18787517371282	20.246310983378738
29	20.64661887210796	26.57368707261393	30.987109129741476	21.792584925536637
30	22.351986171277556	28.155215850490073	29.224945291955752	20.267852686276623
31	27.46272891343125	26.571112576413935	25.989906924081257	19.976251586073552
32	28.403155596770844	26.38816572399825	26.143956370170525	19.06472230906039
33	27.672839327793785	26.540533866446697	25.659215514229345	20.12741129153017
34	22.270548026175796	27.296962882595093	28.26161084651011	22.170878244719
35	22.05786311561327	26.978408383400275	28.823534047712247	22.140194453274205
36	28.005895070890592	26.69132578673189	25.717903519441386	19.584875622936135
37	23.137680378923807	30.278071859968424	27.018076641175654	19.566171119932118
38	23.402328080135135	30.06128877178142	24.535053867392428	22.001329280691014
39	22.610381524574617	28.877178142001853	26.288233238847567	22.224207094575956
40	24.716266875428534	25.511313334576798	26.15141715507662	23.62100263491805
41	20.423951221178122	25.617077841731533	27.44265835121908	26.516312585871272
42	23.00469976908345	26.369829006165656	25.261061795789914	25.364409428960982
43	22.40310831010595	26.559133287973165	26.37839314658603	24.659365255334855
44	21.464730715578593	28.131887762473827	27.468088068786333	22.935293453161247
45	21.42931825764402	30.431123032020952	24.433019752690743	23.706538957644284
46	22.87103612963902	28.85248399477745	26.491881142340745	21.784598733242788
47	22.700961758223283	26.851627580735414	26.997007804926742	23.45040285611456
48	23.322833942368064	25.72420840809443	28.924622429115978	22.028335220421535
49	23.107364372650444	24.485613032204846	29.526844376958785	22.880178218185925
50	21.675891944716636	28.663494957402598	27.279361735105358	22.381251362775412
51	20.787690755719716	29.447455320669263	26.429147500243	23.33570642336802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	948.0
1	1584.0
2	2220.0
3	5530.5
4	8841.0
5	6983.0
6	5125.0
7	5063.0
8	5001.0
9	5318.0
10	5635.0
11	6104.0
12	6573.0
13	6751.0
14	6929.0
15	6630.5
16	6332.0
17	6152.5
18	5973.0
19	6108.5
20	6244.0
21	6592.5
22	6941.0
23	8457.0
24	9973.0
25	11461.0
26	14498.0
27	16047.0
28	19674.5
29	23302.0
30	27534.5
31	31767.0
32	35453.5
33	39140.0
34	43472.5
35	47805.0
36	51769.0
37	55733.0
38	65744.5
39	75756.0
40	90278.0
41	104800.0
42	120786.5
43	136773.0
44	144064.5
45	151356.0
46	151249.0
47	151142.0
48	150890.0
49	150638.0
50	145720.0
51	140802.0
52	131100.0
53	121398.0
54	113078.0
55	104758.0
56	101521.0
57	98284.0
58	96100.5
59	93917.0
60	87687.0
61	81457.0
62	70649.0
63	59841.0
64	52748.0
65	45655.0
66	38378.0
67	31101.0
68	25419.0
69	19737.0
70	16011.0
71	12285.0
72	10341.5
73	8398.0
74	6692.5
75	4205.5
76	3424.0
77	2496.0
78	1568.0
79	1253.0
80	938.0
81	689.5
82	441.0
83	325.5
84	210.0
85	147.0
86	84.0
87	55.5
88	27.0
89	21.0
90	15.0
91	12.5
92	10.0
93	5.5
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1903285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.95838301809225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43277610345893	16.05308152681291
2	7.137647314464106	2.849117979002643
3	2.260456245943105	1.3534515465651427
4	1.0809020334336374	0.8629222715321316
5	0.6848390677032894	0.683414020948773
6	0.4987115813441557	0.5972086053615078
7	0.3473910865596846	0.48533550538405973
8	0.28030977734628804	0.44756239199947
9	0.23927320961398169	0.42979557270997076
>10	3.842731225147056	20.624824830166975
>50	2.4081205455850685	34.6820459287055
>100	0.7814653706638488	19.866022851684182
>500	0.0037635071158467247	0.4881305522874152
>1k	0.0016129316210771675	0.5770864168392436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4494	0.23611808005632368	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027636428595822486	0.0
2	0.0	0.0	0.0	0.11338291427715765	0.0
3	0.0	0.0	0.0	0.1708624824973664	0.0
4	0.0	0.0	0.0	0.3396233354437197	0.0
5	0.0	0.0	0.0	0.5923442889530469	0.0
6	0.0	0.0	0.0	0.9070633142172612	0.0
7	0.0	0.0	0.0	1.0527062421024702	0.0
8	0.0	0.0	0.0	1.4130306286236691	0.0
9	0.0	0.0	0.0	1.5181646469131003	0.0
10	0.0	0.0	0.0	1.7450355569449663	0.0
11	0.0	0.0	0.0	2.078248922258096	0.0
12	0.0	0.0	0.0	2.3364866533388327	0.0
13	0.0	0.0	0.0	2.444195167828255	0.0
14	0.0	0.0	0.0	2.4890124180036097	0.0
15	0.0	0.0	0.0	2.555739156248276	0.0
16	0.0	0.0	0.0	2.716461276162004	0.0
17	0.0	0.0	0.0	2.8910541511124186	0.0
18	0.0	0.0	0.0	3.114930239034091	0.0
19	0.0	0.0	0.0	3.2446007823316005	0.0
20	0.0	0.0	0.0	3.3827829253107127	0.0
21	0.0	0.0	0.0	3.561421437146828	0.0
22	0.0	0.0	0.0	3.741215845236	0.0
23	0.0	0.0	0.0	3.945231533900598	0.0
24	0.0	0.0	0.0	4.0936591209409	0.0
25	0.0	0.0	0.0	4.229582012152673	0.0
26	0.0	0.0	0.0	4.3567831407277415	0.0
27	0.0	0.0	0.0	4.490499320910951	0.0
28	0.0	0.0	0.0	4.634881271065552	0.0
29	0.0	0.0	0.0	4.793974628077246	0.0
30	0.0	0.0	0.0	4.999619079643879	0.0
31	0.0	0.0	0.0	5.190394502137095	0.0
32	0.0	0.0	0.0	5.351064081312047	0.0
33	0.0	0.0	0.0	5.524816304442057	0.0
34	0.0	0.0	0.0	5.7133850159067086	0.0
35	0.0	0.0	0.0	5.94346091100387	0.0
36	0.0	0.0	0.0	6.125987437509359	0.0
37	0.0	0.0	0.0	6.31849670438216	0.0
38	0.0	0.0	0.0	6.5370661776875245	0.0
39	0.0	0.0	0.0	6.874062476192478	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	95	0.0	45.000004	1
GGTACGC	20	7.034456E-4	45.0	8
CGTAACA	20	7.034456E-4	45.0	35
CCGGTTA	35	1.2124838E-7	45.0	31
GCGAATA	30	2.1662563E-6	44.999996	1
CGTACGA	30	2.1662563E-6	44.999996	36
TCATACG	30	2.1662563E-6	44.999996	1
GCGTAAT	30	2.1662563E-6	44.999996	32
TCGAACG	85	0.0	42.35294	1
TATCGCG	70	0.0	41.785713	1
CGGTCTA	245	0.0	41.32653	31
TACGTAG	135	0.0	40.000004	1
TACGCGG	90	0.0	40.0	2
CGTAAGG	440	0.0	39.886364	2
AGGGCGA	1805	0.0	39.39058	6
TACGGGA	785	0.0	39.267517	4
GGCGATA	745	0.0	38.959732	8
CTACGGG	480	0.0	38.906246	3
ACGGGAT	855	0.0	38.68421	5
GGGCGAT	3205	0.0	38.681747	7
>>END_MODULE