FastQCFastQC Report
Thu 26 May 2016
SRR1547982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547982_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2408966
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293821.2196934286328656No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27370.11361721169995757No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGATA207.034828E-445.015
CGTTTTT167250.042.2287031
TAGGGCG7850.039.554145
CGGTTTT16100.038.5714261
TAAGGGA35150.038.4708374
TAGGGAC27850.038.2944375
AGGGCGA19750.038.164566
TACGGGA10300.038.009714
TAGGGAT43700.037.997715
TTAGGGA36300.037.8719024
CGACCAA4100.037.86585229
GGGCGAT40050.037.696637
ACGGGAT11250.037.65
GTACGCG601.5643309E-1037.4999961
ATAGGGA40700.037.4262924
AAGGGAT42750.037.315795
AAGGGAC29500.037.2966085
GTAGGGA37050.037.226724
AGGGATT51750.037.2173926
TAGGGAG29950.036.886485