##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547982_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2408966
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.870431131032984	31.0	31.0	31.0	30.0	33.0
2	31.169833862329316	31.0	31.0	33.0	30.0	34.0
3	31.12949995973376	31.0	31.0	34.0	28.0	34.0
4	35.13680765938581	35.0	35.0	37.0	32.0	37.0
5	35.05702529633046	35.0	35.0	37.0	32.0	37.0
6	35.12264058521374	37.0	35.0	37.0	32.0	37.0
7	35.56198800647249	36.0	35.0	37.0	35.0	37.0
8	35.66466359425579	37.0	35.0	37.0	35.0	37.0
9	37.47594445085568	39.0	37.0	39.0	35.0	39.0
10	36.63631159592954	39.0	37.0	39.0	32.0	39.0
11	36.220874848378934	38.0	35.0	39.0	31.0	39.0
12	35.94547204070128	38.0	35.0	39.0	31.0	39.0
13	35.842409149817804	38.0	35.0	39.0	30.0	39.0
14	36.84889699564045	39.0	35.0	41.0	31.0	41.0
15	36.98608033488227	39.0	35.0	41.0	31.0	41.0
16	37.04672129037936	39.0	35.0	41.0	31.0	41.0
17	36.96699496796551	39.0	35.0	41.0	31.0	41.0
18	36.79385263220818	39.0	35.0	40.0	31.0	41.0
19	36.68855309705492	38.0	35.0	40.0	31.0	41.0
20	36.48858431376782	38.0	35.0	40.0	30.0	41.0
21	36.39189967811916	38.0	35.0	40.0	30.0	41.0
22	36.300326779207346	38.0	35.0	40.0	30.0	41.0
23	36.21671621766351	38.0	34.0	40.0	30.0	41.0
24	36.08623160310274	38.0	34.0	40.0	30.0	41.0
25	35.93115469458681	38.0	34.0	40.0	30.0	41.0
26	35.71129480449288	38.0	34.0	40.0	29.0	41.0
27	35.78352413442116	38.0	34.0	40.0	29.0	41.0
28	35.77108311200739	38.0	34.0	40.0	29.0	41.0
29	35.72924275394505	38.0	34.0	40.0	29.0	41.0
30	35.6086424631979	38.0	34.0	40.0	29.0	41.0
31	35.463958395427746	38.0	34.0	40.0	27.0	41.0
32	35.26395142148125	38.0	34.0	40.0	27.0	41.0
33	34.980497441640935	38.0	33.0	40.0	25.0	41.0
34	34.63741206808232	38.0	33.0	40.0	24.0	41.0
35	34.37937355695348	38.0	33.0	40.0	23.0	41.0
36	34.27815668631272	38.0	33.0	40.0	22.0	41.0
37	34.21643559934013	38.0	33.0	40.0	22.0	41.0
38	34.123360811236026	37.0	33.0	40.0	21.0	41.0
39	34.06427820068859	37.0	33.0	40.0	21.0	41.0
40	33.91332173222868	37.0	33.0	40.0	20.0	41.0
41	33.81157019235639	37.0	33.0	40.0	20.0	41.0
42	33.77416866821699	37.0	33.0	40.0	19.0	41.0
43	33.72534232529642	37.0	33.0	40.0	19.0	41.0
44	33.575236429239766	37.0	32.0	40.0	18.0	41.0
45	33.4690767740184	37.0	32.0	40.0	19.0	41.0
46	33.42257964620505	37.0	32.0	40.0	19.0	41.0
47	33.370500040266236	36.0	32.0	40.0	19.0	41.0
48	33.25542784746651	36.0	32.0	40.0	18.0	41.0
49	33.121370330672995	36.0	31.0	40.0	18.0	41.0
50	33.02854959347704	36.0	31.0	40.0	18.0	41.0
51	32.73337772305628	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	2.0
12	4.0
13	9.0
14	43.0
15	116.0
16	327.0
17	789.0
18	1758.0
19	3369.0
20	5644.0
21	8820.0
22	13253.0
23	19313.0
24	28493.0
25	42109.0
26	55066.0
27	61733.0
28	63926.0
29	68770.0
30	76937.0
31	90384.0
32	107652.0
33	133287.0
34	181081.0
35	205430.0
36	206905.0
37	254789.0
38	348790.0
39	430120.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.026990833411517	23.272308534034934	28.265239110888242	18.43546152166531
2	33.88130010967361	25.359303535209715	27.822850135701376	12.936546219415302
3	27.574029687426055	25.436847178415967	34.019658226807685	12.96946490735029
4	24.584987915977226	27.535506935340727	33.81135308676004	14.068152061922005
5	22.37258641259362	31.237842294162725	32.62623880951413	13.763332483729535
6	21.194653639777396	40.05095962334047	29.1055996639222	9.648787072959934
7	86.80176474055673	2.479404026457825	9.549242289015288	1.1695889439701517
8	87.42302714110536	2.006337988996109	9.376055950976477	1.1945789189220604
9	82.54545726257656	4.887657193999417	10.548965821850537	2.0179197215734885
10	36.819573211078946	37.963923110579394	15.752899791860905	9.463603886480755
11	25.786250200293402	25.07478312271738	32.17073217305682	16.968234503932393
12	26.619595295242853	22.574374233592337	32.74458834205215	18.061442129112656
13	23.896144652934083	24.03462730482705	33.41969127003038	18.649536772208492
14	18.60474577059203	27.124168626705398	34.12696567739022	20.144119925312353
15	18.51578644115359	26.88369200727615	37.319912360739	17.280609190831253
16	23.578290436643773	25.47516237256981	34.802732790749225	16.143814400037193
17	22.76150846462756	25.1609196642875	32.473559195106944	19.60401267597799
18	22.257806876477293	25.668149737273172	33.96984432324906	18.104199063000472
19	24.169415425539423	27.042805917559647	31.048757018571454	17.739021638329476
20	25.10272042029651	27.70520630013043	30.803880170994525	16.388193108578538
21	24.715583366473417	25.627509894286593	32.710258260183004	16.94664847905699
22	23.21390173211245	23.875015255507964	33.5997270198085	19.311355992571087
23	21.12445754734604	26.43104967027347	33.409770000904956	19.034722781475537
24	20.559526369404963	26.77518072069095	34.253243922911324	18.41204898699276
25	21.859918321802798	27.513007655566746	31.593887169847978	19.033186852782478
26	20.637651174819403	28.947938659159156	30.766602766498156	19.64780739952328
27	19.551583542482543	28.11094054461541	33.10930913927386	19.22816677362819
28	18.959171694411626	28.141119467854676	33.85161932546993	19.04808951226377
29	20.66205998756313	27.193783556928576	33.14476003397308	18.999396421535213
30	21.962244382029468	25.94515655264541	33.16182129594191	18.930777769383212
31	22.73299000484025	26.818892421063644	31.603393323110414	18.844724250985692
32	23.6712348783669	26.928732078410405	31.36225251830038	18.03778052492231
33	23.242295657140865	27.497938949740263	30.636879059314243	18.62288633380463
34	21.164308670192938	28.01106366798037	32.026603945427205	18.798023716399484
35	20.878335352180148	28.583259373523745	31.069222230616788	19.469183043679323
36	22.035055704397656	29.436114914033656	29.62511716645233	18.90371221511636
37	21.61545659008886	30.41030882129511	29.362141267249104	18.612093321366928
38	21.557838508306055	29.767169814766998	29.081813524972954	19.593178151953992
39	21.09515036741905	28.9212051975827	29.263841830893423	20.71980260410483
40	22.445480758134405	28.076818020677752	29.740311818431643	19.737389402756204
41	19.939675362790506	28.74860002175207	30.124003410591932	21.18772120486549
42	20.849484799702445	28.927141354423437	29.530595284449845	20.69277856142428
43	20.92736053559909	28.452788457786454	29.547075384210487	21.07277562240397
44	20.85222456439817	29.146986715462152	29.369945445473284	20.63084327466639
45	20.48891516111062	29.19119655487043	28.767280235586558	21.552608048432397
46	20.699877042681383	29.613701480220143	29.035777175767528	20.650644301330946
47	20.40518629154583	28.957071208144907	30.22226133536131	20.41548116494795
48	20.743671766226672	28.26046527846387	30.65028730168877	20.34557565362068
49	21.181411443748065	27.87473961857494	30.155386169833864	20.788462767843132
50	19.68292620153211	29.130133011424817	30.374816415009594	20.812124372033477
51	19.4229391365424	29.19700817695227	30.181663003961035	21.198389682544295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1173.0
1	1965.5
2	2758.0
3	26809.5
4	50861.0
5	38131.5
6	25402.0
7	23139.0
8	20876.0
9	21269.0
10	21662.0
11	21730.0
12	21798.0
13	21643.5
14	21489.0
15	20416.5
16	19344.0
17	18385.5
18	17427.0
19	17033.5
20	16640.0
21	17407.5
22	18175.0
23	18609.0
24	19043.0
25	21518.5
26	28354.0
27	32714.0
28	37914.5
29	43115.0
30	50149.5
31	57184.0
32	63701.0
33	70218.0
34	78461.0
35	86704.0
36	92486.0
37	98268.0
38	106241.0
39	114214.0
40	122452.0
41	130690.0
42	137400.0
43	144110.0
44	150283.0
45	156456.0
46	161677.0
47	166898.0
48	166247.0
49	165596.0
50	160390.0
51	155184.0
52	145888.0
53	136592.0
54	127453.5
55	118315.0
56	112731.0
57	107147.0
58	101240.0
59	95333.0
60	87187.5
61	79042.0
62	69551.5
63	60061.0
64	50143.0
65	40225.0
66	33098.0
67	25971.0
68	21215.0
69	16459.0
70	13555.0
71	10651.0
72	8954.0
73	7257.0
74	5773.0
75	3547.5
76	2806.0
77	2099.5
78	1393.0
79	1106.5
80	820.0
81	581.0
82	342.0
83	234.5
84	127.0
85	99.0
86	71.0
87	55.0
88	39.0
89	28.5
90	18.0
91	14.5
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2408966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.830853732490493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91195822409959	18.31716420771193
2	6.962337934844563	3.5968626566227964
3	3.285103084642113	2.5457105182663127
4	2.215370237966421	2.288996183208931
5	1.6110390609149976	2.0807257169912075
6	1.269053904830218	1.9668447476589161
7	1.0591959871109253	1.915195563197224
8	0.952462177679052	1.968232895788557
9	0.8247189596856962	1.9172875336247648
>10	10.67553006182176	57.44733387798671
>50	0.20925622531334123	3.272719152163587
>100	0.022004171812256758	1.012339950706177
>500	0.0016416410659059728	0.30858111408321154
>1k	1.6416410659059728E-4	0.1160624583270235
>5k	0.0	0.0
>10k+	1.6416410659059728E-4	1.2459434236626248
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29382	1.2196934286328656	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2737	0.11361721169995757	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.151158629885187E-5	0.0	0.0	0.013657311892322267	0.0
2	4.151158629885187E-5	0.0	0.0	0.04748925472588654	0.0
3	4.151158629885187E-5	0.0	0.0	0.09352560393131328	0.0
4	4.151158629885187E-5	0.0	0.0	0.13856567506556755	0.0
5	4.151158629885187E-5	0.0	0.0	0.27185937867118093	0.0
6	4.151158629885187E-5	0.0	0.0	0.5647651315958797	0.0
7	4.151158629885187E-5	0.0	0.0	0.7766817796515185	0.0
8	4.151158629885187E-5	0.0	0.0	1.109438655423115	0.0
9	4.151158629885187E-5	0.0	0.0	1.38694360983094	0.0
10	4.151158629885187E-5	0.0	0.0	1.7034694553596854	0.0
11	4.151158629885187E-5	0.0	0.0	1.9605091977221762	0.0
12	4.151158629885187E-5	0.0	0.0	2.1459414537191477	0.0
13	4.151158629885187E-5	0.0	0.0	2.2642494746708755	0.0
14	4.151158629885187E-5	0.0	0.0	2.3633791427525335	0.0
15	4.151158629885187E-5	0.001660463451954075	0.0	2.434239420564674	0.0
16	4.151158629885187E-5	0.001660463451954075	0.0	2.514024689431067	0.0
17	4.151158629885187E-5	0.001660463451954075	0.0	2.605640760392633	0.0
18	4.151158629885187E-5	0.001660463451954075	0.0	2.7234506423087748	0.0
19	4.151158629885187E-5	0.001660463451954075	0.0	2.806971953942065	0.0
20	4.151158629885187E-5	0.001660463451954075	0.0	2.8998333724925964	0.0
21	4.151158629885187E-5	0.001660463451954075	0.0	2.9939816502183922	0.0
22	4.151158629885187E-5	0.001660463451954075	0.0	3.110878277235959	0.0
23	4.151158629885187E-5	0.001660463451954075	0.0	3.236243267858492	0.0
24	4.151158629885187E-5	0.001660463451954075	0.0	3.3398146756741274	0.0
25	4.151158629885187E-5	0.001660463451954075	0.0	3.4359970211285673	0.0
26	4.151158629885187E-5	0.001660463451954075	0.0	3.5342964574842486	0.0
27	4.151158629885187E-5	0.001660463451954075	0.0	3.645505997178873	0.0
28	4.151158629885187E-5	0.001660463451954075	0.0	3.7505718221012665	0.0
29	4.151158629885187E-5	0.001660463451954075	0.0	3.8729894900965807	0.0
30	4.151158629885187E-5	0.001660463451954075	0.0	4.019276320213735	0.0
31	4.151158629885187E-5	0.001660463451954075	0.0	4.15829862272859	0.0
32	4.151158629885187E-5	0.001660463451954075	0.0	4.295369880687399	0.0
33	4.151158629885187E-5	0.001660463451954075	0.0	4.424304867731633	0.0
34	4.151158629885187E-5	0.001660463451954075	0.0	4.565527284320327	0.0
35	4.151158629885187E-5	0.001660463451954075	0.0	4.715218064513986	0.0
36	4.151158629885187E-5	0.001660463451954075	0.0	4.869433607614221	0.0
37	4.151158629885187E-5	0.001660463451954075	0.0	5.029253214864801	0.0
38	7.056969670804818E-4	0.001660463451954075	0.0	5.224274647296807	0.0
39	7.056969670804818E-4	0.001660463451954075	0.0	5.519463537467943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGATA	20	7.034828E-4	45.0	15
CGTTTTT	16725	0.0	42.228703	1
TAGGGCG	785	0.0	39.55414	5
CGGTTTT	1610	0.0	38.571426	1
TAAGGGA	3515	0.0	38.470837	4
TAGGGAC	2785	0.0	38.294437	5
AGGGCGA	1975	0.0	38.16456	6
TACGGGA	1030	0.0	38.00971	4
TAGGGAT	4370	0.0	37.99771	5
TTAGGGA	3630	0.0	37.871902	4
CGACCAA	410	0.0	37.865852	29
GGGCGAT	4005	0.0	37.69663	7
ACGGGAT	1125	0.0	37.6	5
GTACGCG	60	1.5643309E-10	37.499996	1
ATAGGGA	4070	0.0	37.426292	4
AAGGGAT	4275	0.0	37.31579	5
AAGGGAC	2950	0.0	37.296608	5
GTAGGGA	3705	0.0	37.22672	4
AGGGATT	5175	0.0	37.217392	6
TAGGGAG	2995	0.0	36.88648	5
>>END_MODULE