FastQCFastQC Report
Thu 26 May 2016
SRR1547981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547981_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3043072
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81700.2684786952132582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA556.184564E-1140.90909231
ACGGGAT14050.040.5160145
CGTTTTT58300.039.558321
TACGGGA13000.038.769234
AATGCGG4350.038.275862
TGCGTAG1300.038.076921
TAGCACG1550.037.741941
AGGGATC35600.037.605346
GGCGATT14400.037.58
CGTACGT601.5643309E-1037.49999613
TAGGGAT44250.037.423735
GGGCGAT51650.037.4201357
AGGGATT56600.037.407246
TTAGGGA40900.037.1882634
AAGGGAT56400.036.9015965
TGGGCGA15450.036.8446586
CGGGATA7400.036.7905436
TAAGGGA44200.036.702494
GTAGGGA41250.036.6000024
TAGGGAC33350.036.2968525
Produced by FastQC (version 0.11.5)