##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547981_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3043072
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.801706630667958	31.0	31.0	31.0	30.0	33.0
2	31.146669221102886	31.0	31.0	33.0	30.0	34.0
3	31.339169760031968	31.0	31.0	34.0	30.0	34.0
4	35.194556027593165	35.0	35.0	37.0	33.0	37.0
5	35.01177001398587	35.0	35.0	37.0	32.0	37.0
6	35.03216650805502	36.0	35.0	37.0	32.0	37.0
7	35.476150087806005	35.0	35.0	37.0	35.0	37.0
8	35.555575747139734	37.0	35.0	37.0	35.0	37.0
9	37.33583431479768	39.0	37.0	39.0	35.0	39.0
10	36.42779894790527	38.0	35.0	39.0	32.0	39.0
11	36.01649747360562	38.0	35.0	39.0	31.0	39.0
12	35.57873195244805	37.0	35.0	39.0	30.0	39.0
13	35.37509529843526	37.0	35.0	39.0	30.0	39.0
14	36.3459806406158	38.0	35.0	40.0	30.0	41.0
15	36.53375700607807	38.0	35.0	40.0	30.0	41.0
16	36.63792673982081	38.0	35.0	40.0	31.0	41.0
17	36.55564442773618	38.0	35.0	40.0	31.0	41.0
18	36.47235852454362	38.0	35.0	40.0	30.0	41.0
19	36.439555488664084	38.0	35.0	40.0	30.0	41.0
20	36.343572876356525	38.0	34.0	40.0	30.0	41.0
21	36.22550403013797	38.0	34.0	40.0	30.0	41.0
22	36.1065893281526	38.0	34.0	40.0	30.0	41.0
23	36.03517366661058	38.0	34.0	40.0	30.0	41.0
24	35.91729771756961	38.0	34.0	40.0	29.0	41.0
25	35.75300913024733	38.0	34.0	40.0	29.0	41.0
26	35.50073412656684	38.0	34.0	40.0	27.0	41.0
27	35.58473575386977	38.0	34.0	40.0	28.0	41.0
28	35.608514356544966	38.0	34.0	40.0	29.0	41.0
29	35.59637267866156	38.0	34.0	40.0	29.0	41.0
30	35.51652080529149	38.0	34.0	40.0	28.0	41.0
31	35.44989175412215	38.0	34.0	40.0	27.0	41.0
32	35.34355053051653	38.0	34.0	40.0	27.0	41.0
33	35.23109673382687	38.0	34.0	40.0	27.0	41.0
34	35.094022093463444	38.0	34.0	40.0	26.0	41.0
35	34.96365777740389	38.0	33.0	40.0	25.0	41.0
36	34.918982199566756	38.0	33.0	40.0	25.0	41.0
37	34.89063650153529	38.0	33.0	40.0	25.0	41.0
38	34.81337838868091	37.0	33.0	40.0	25.0	41.0
39	34.77797436274922	37.0	33.0	40.0	25.0	41.0
40	34.595713804997054	37.0	33.0	40.0	24.0	41.0
41	34.51009341875579	37.0	33.0	40.0	24.0	41.0
42	34.479662985299065	37.0	33.0	40.0	24.0	41.0
43	34.43244721123917	37.0	33.0	40.0	24.0	41.0
44	34.3078793403508	37.0	33.0	40.0	23.0	41.0
45	34.17785908450408	37.0	33.0	40.0	23.0	41.0
46	34.08923285416842	36.0	33.0	40.0	23.0	41.0
47	34.0286900868596	36.0	33.0	40.0	23.0	41.0
48	33.93572482018171	36.0	33.0	40.0	23.0	41.0
49	33.790903731492385	36.0	32.0	40.0	23.0	41.0
50	33.66749718705308	36.0	32.0	40.0	23.0	41.0
51	33.313821690712544	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	19.0
14	64.0
15	148.0
16	407.0
17	902.0
18	1936.0
19	3614.0
20	6259.0
21	9755.0
22	14614.0
23	21015.0
24	29539.0
25	39762.0
26	51260.0
27	61135.0
28	71743.0
29	85645.0
30	103070.0
31	123647.0
32	148736.0
33	184238.0
34	259900.0
35	291439.0
36	264390.0
37	321318.0
38	431563.0
39	516891.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.604035658702784	25.437781294691682	28.729192079582738	12.2289909670228
2	30.063994542357197	26.896175969546565	29.416720997728614	13.623108490367628
3	29.8581827837133	26.225340708336837	30.167508359973077	13.74896814797678
4	25.692326701438546	29.29904386094052	29.498776236645075	15.509853200975856
5	23.56013922772777	33.431216875578365	28.160621897871625	14.848021998822242
6	22.557928304029613	41.849124831748966	25.415534039286612	10.177412824934803
7	90.70787677715151	3.0486626671994617	4.836625620425675	1.4068349352233533
8	90.64317242575923	2.716925527887608	4.585727843442416	2.054174202910743
9	85.1351857596534	5.889016099520485	6.323806995036595	2.651991145789518
10	40.60002523765458	36.72197042988138	11.960216518044923	10.717787814419113
11	31.958626020021875	25.623580381929838	25.63080991839825	16.78698367965004
12	31.14895079708926	23.394812873306975	27.39563835492555	18.06059797467822
13	24.981597543534953	28.391539864978547	28.278496203836124	18.348366387650376
14	19.005005468158494	31.461628249348028	29.475247381593338	20.058118900900144
15	18.89945423571969	27.903710461007826	35.461927946496175	17.73490735677631
16	22.85614668335156	26.30611434760663	33.15508144401447	17.68265752502734
17	22.495524259695465	26.013350982165388	28.09203988600993	23.399084872129215
18	23.28495678051653	26.689969872549845	30.44663419071254	19.57843915622108
19	25.46219741103727	28.02059892109027	26.394741892403466	20.122461775469
20	27.478482270547655	27.802004027509042	26.06671810591402	18.652795596029275
21	25.304494931437706	27.583803472280643	28.978315333978294	18.133386262303354
22	24.343886703962312	24.946238537898545	28.506456633296878	22.203418124842266
23	22.098754153697318	28.764288192983933	28.111066711533606	21.025890941785143
24	21.80622081896189	27.041161037267603	31.528764353916046	19.62385378985446
25	21.536230493396147	28.69320870488769	28.56711901657273	21.203441785143433
26	20.93292567510726	31.51591549591991	26.47594273155548	21.075216097417346
27	20.42741676831833	30.04956833094978	28.817491009085554	20.705523891646337
28	19.301843663245563	29.689866030116935	30.65224878018003	20.356041526457474
29	20.149835429460754	27.339379416589555	30.730590666274082	21.78019448767561
30	21.552957011861697	28.234560339025826	29.718981345166988	20.493501303945486
31	23.54347843232102	28.326046836880625	27.45265310843779	20.67782162236056
32	24.718738169849416	28.074524690838732	27.14194734794313	20.064789791368725
33	23.79260168671658	27.995525574156645	27.128506982417765	21.08336575670901
34	20.577988296037688	27.984286931101206	29.65953483847901	21.778189934382098
35	20.871277445949357	27.699213163540005	29.680467632708	21.749041757802644
36	23.837950597291158	28.6106276815008	27.331032588121477	20.220389133086567
37	21.216323504668967	29.562067542273073	28.45893886178178	20.762670091276185
38	21.588447463615715	29.841324819130143	27.134290611592498	21.435937105661647
39	21.125329929755196	28.862149827542694	28.022833505089594	21.98968673761252
40	23.756749758139144	26.854836165559014	27.905353537477918	21.483060538823924
41	19.938470072347943	26.619449030453435	29.067304355598555	24.374776541600067
42	21.650818646420458	27.71837143518129	26.831307310507274	23.799502607890975
43	21.24047672877934	27.2082619142761	28.145571317405572	23.405690039538992
44	20.797503312442164	28.634879490199378	27.65853059014049	22.90908660721797
45	20.44394611760747	28.93385368469757	27.264455129553294	23.357745068141668
46	21.482666200471105	29.2599386409523	27.37878039034239	21.878614768234208
47	20.8350640405485	27.841897924202915	29.01380578573231	22.309232249516278
48	21.574382729031715	26.9178317174224	29.620298172373182	21.88748738117271
49	21.469028665769326	26.55359452553209	29.61977238790275	22.357604420795827
50	20.265047951543703	28.654333515605284	29.23785569319425	21.842762839656768
51	20.05069219525532	28.32647403676285	27.585380825691928	24.037452942289896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1264.0
1	2095.0
2	2926.0
3	9187.0
4	15448.0
5	12111.5
6	8775.0
7	8570.0
8	8365.0
9	9023.0
10	9681.0
11	10241.0
12	10801.0
13	11310.0
14	11819.0
15	11669.5
16	11520.0
17	11573.0
18	11626.0
19	11840.0
20	12054.0
21	13437.5
22	14821.0
23	17533.5
24	20246.0
25	23719.0
26	32728.0
27	38264.0
28	45775.5
29	53287.0
30	59993.5
31	66700.0
32	76715.0
33	86730.0
34	98002.5
35	109275.0
36	113953.0
37	118631.0
38	130842.0
39	143053.0
40	162015.5
41	180978.0
42	198164.0
43	215350.0
44	226433.5
45	237517.0
46	239829.5
47	242142.0
48	244820.0
49	247498.0
50	234564.0
51	221630.0
52	204173.0
53	186716.0
54	172032.0
55	157348.0
56	143952.0
57	130556.0
58	122049.0
59	113542.0
60	106975.0
61	100408.0
62	87838.0
63	75268.0
64	65109.0
65	54950.0
66	45504.5
67	36059.0
68	29350.0
69	22641.0
70	18681.0
71	14721.0
72	11727.0
73	8733.0
74	7817.5
75	5127.5
76	3353.0
77	2955.0
78	2557.0
79	1720.5
80	884.0
81	650.5
82	417.0
83	297.0
84	177.0
85	156.5
86	136.0
87	88.5
88	41.0
89	35.5
90	30.0
91	26.0
92	22.0
93	17.0
94	12.0
95	9.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3043072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.340915779256413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65779318516705	16.786293397468192
2	8.046835055175519	3.4345365840413784
3	2.7499151008562985	1.7605711970243902
4	1.36451695695782	1.1648016583121634
5	0.8403276014953147	0.8966680285248031
6	0.5837280535667833	0.7474374737494798
7	0.4534232623849752	0.6773527358439406
8	0.3566116335851263	0.6088335070594584
9	0.29689991052511955	0.5702504386846806
>10	3.8618069739117957	20.92411526798393
>50	2.0667780829445745	31.98674810220489
>100	0.7164582897736808	19.308092028342443
>500	0.0037981112175463215	0.5864003936384308
>1k	9.495278043865804E-4	0.271973873752824
>5k	1.5825463406443006E-4	0.2759253133690019
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8170	0.2684786952132582	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.286152940186759E-5	0.0	0.0	0.045447495162782875	0.0
2	3.286152940186759E-5	0.0	0.0	0.1729502292420291	0.0
3	3.286152940186759E-5	0.0	0.0	0.2673614032135947	0.0
4	3.286152940186759E-5	0.0	0.0	0.4686382708000337	0.0
5	3.286152940186759E-5	0.0	0.0	0.8734594515016404	0.0
6	3.286152940186759E-5	0.0	0.0	1.4293450828636325	0.0
7	3.286152940186759E-5	0.0	0.0	1.7000583620762177	0.0
8	3.286152940186759E-5	0.0	0.0	2.3331357260031966	0.0
9	3.286152940186759E-5	0.0	0.0	2.555969756877261	0.0
10	3.286152940186759E-5	0.0	0.0	2.937623559350551	0.0
11	3.286152940186759E-5	0.0	0.0	3.4095479830907713	0.0
12	3.286152940186759E-5	0.0	0.0	3.77815575839152	0.0
13	3.286152940186759E-5	0.0	0.0	3.9527490641036427	0.0
14	3.286152940186759E-5	0.0	0.0	4.03786042525448	0.0
15	3.286152940186759E-5	0.0	0.0	4.143707411457895	0.0
16	3.286152940186759E-5	0.0	0.0	4.35546710692353	0.0
17	3.286152940186759E-5	0.0	0.0	4.600745562379069	0.0
18	3.286152940186759E-5	0.0	0.0	4.884669176411205	0.0
19	3.286152940186759E-5	0.0	0.0	5.062548635063515	0.0
20	3.286152940186759E-5	0.0	0.0	5.253638428535375	0.0
21	3.286152940186759E-5	0.0	0.0	5.494020516110036	0.0
22	3.286152940186759E-5	0.0	0.0	5.762630657440901	0.0
23	3.286152940186759E-5	0.0	0.0	6.037353043240515	0.0
24	3.286152940186759E-5	0.0	0.0	6.247699692941869	0.0
25	3.286152940186759E-5	0.0	0.0	6.441385547236477	0.0
26	3.286152940186759E-5	0.0	0.0	6.624818604357702	0.0
27	3.286152940186759E-5	0.0	0.0	6.81534975182973	0.0
28	3.286152940186759E-5	0.0	0.0	7.009298498359552	0.0
29	3.286152940186759E-5	0.0	0.0	7.224870131235804	0.0
30	3.286152940186759E-5	0.0	0.0	7.48434476739295	0.0
31	3.286152940186759E-5	0.0	0.0	7.705469998738117	0.0
32	3.286152940186759E-5	0.0	0.0	7.933233259022462	0.0
33	3.286152940186759E-5	0.0	0.0	8.153766982838395	0.0
34	3.286152940186759E-5	0.0	0.0	8.37712679818289	0.0
35	3.286152940186759E-5	0.0	0.0	8.637784449398502	0.0
36	3.286152940186759E-5	0.0	0.0	8.87054266215193	0.0
37	3.286152940186759E-5	0.0	0.0	9.116576932783714	0.0
38	3.286152940186759E-5	0.0	0.0	9.37923913729284	0.0
39	3.286152940186759E-5	0.0	0.0	9.711929260957348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	55	6.184564E-11	40.909092	31
ACGGGAT	1405	0.0	40.516014	5
CGTTTTT	5830	0.0	39.55832	1
TACGGGA	1300	0.0	38.76923	4
AATGCGG	435	0.0	38.27586	2
TGCGTAG	130	0.0	38.07692	1
TAGCACG	155	0.0	37.74194	1
AGGGATC	3560	0.0	37.60534	6
GGCGATT	1440	0.0	37.5	8
CGTACGT	60	1.5643309E-10	37.499996	13
TAGGGAT	4425	0.0	37.42373	5
GGGCGAT	5165	0.0	37.420135	7
AGGGATT	5660	0.0	37.40724	6
TTAGGGA	4090	0.0	37.188263	4
AAGGGAT	5640	0.0	36.901596	5
TGGGCGA	1545	0.0	36.844658	6
CGGGATA	740	0.0	36.790543	6
TAAGGGA	4420	0.0	36.70249	4
GTAGGGA	4125	0.0	36.600002	4
TAGGGAC	3335	0.0	36.296852	5
>>END_MODULE