FastQCFastQC Report
Thu 26 May 2016
SRR1547980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547980_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2190153
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.19153912991466807No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30090.1373876619578632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG2650.042.4528326
CGCGTAA650.041.53846431
CGGGTAT1750.041.1428576
CGGTCTA2750.040.90909231
TCGCGCG501.0822987E-940.51
ACGGGTA2750.040.0909085
GGGCGAT34550.039.7250377
ACAACGA4850.039.4329913
AGTACGG1950.039.230772
CGACGGT2900.038.79310628
ACGGGAT7000.038.5714265
TCGATCA1700.038.3823517
TCACGAC3000.038.2525
GCGATAC659.094947E-1238.0769279
GCGATCT1950.038.0769239
TGGGCGA11250.038.06
AGGGCGA18150.037.9338846
TATAGCG950.037.8947371
GGCACCG7750.037.7419368
TACGGGA8300.037.680724
Produced by FastQC (version 0.11.5)