##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547980_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2190153
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88547375457331	31.0	31.0	31.0	30.0	33.0
2	31.239998758077633	31.0	31.0	33.0	30.0	34.0
3	31.45719682597517	31.0	31.0	34.0	30.0	34.0
4	35.31342422196075	37.0	35.0	37.0	33.0	37.0
5	35.134956324969075	37.0	35.0	37.0	32.0	37.0
6	35.15480105727773	37.0	35.0	37.0	32.0	37.0
7	35.513232637171924	35.0	35.0	37.0	35.0	37.0
8	35.59251750905074	37.0	35.0	37.0	35.0	37.0
9	37.41745531019979	39.0	37.0	39.0	35.0	39.0
10	36.50950687006798	38.0	35.0	39.0	32.0	39.0
11	36.16869871648237	38.0	35.0	39.0	31.0	39.0
12	35.614662537274796	37.0	35.0	39.0	30.0	39.0
13	35.33071296845471	37.0	35.0	39.0	30.0	39.0
14	36.26562299528846	38.0	35.0	40.0	30.0	41.0
15	36.503308672955725	38.0	35.0	40.0	30.0	41.0
16	36.636734054652806	38.0	35.0	40.0	31.0	41.0
17	36.582170286733394	38.0	35.0	40.0	31.0	41.0
18	36.5015813963682	38.0	35.0	40.0	30.0	41.0
19	36.467577379297246	38.0	35.0	40.0	30.0	41.0
20	36.371211965556746	38.0	35.0	40.0	30.0	41.0
21	36.24647867066821	38.0	34.0	40.0	30.0	41.0
22	36.12381920349857	38.0	34.0	40.0	30.0	41.0
23	36.06478314528711	38.0	34.0	40.0	30.0	41.0
24	35.947160312544376	38.0	34.0	40.0	30.0	41.0
25	35.785984814759516	38.0	34.0	40.0	29.0	41.0
26	35.573395557296685	38.0	34.0	40.0	29.0	41.0
27	35.652435697414745	38.0	34.0	40.0	29.0	41.0
28	35.70545984686915	38.0	34.0	40.0	29.0	41.0
29	35.70832540009762	38.0	34.0	40.0	29.0	41.0
30	35.634948334659725	38.0	34.0	40.0	29.0	41.0
31	35.55404850711343	38.0	34.0	40.0	29.0	41.0
32	35.432918613448464	38.0	34.0	40.0	28.0	41.0
33	35.339262142873125	38.0	34.0	40.0	27.0	41.0
34	35.19731132939114	38.0	34.0	40.0	27.0	41.0
35	35.0742710669072	37.0	34.0	40.0	26.0	41.0
36	35.01862335645044	37.0	34.0	40.0	26.0	41.0
37	34.96386553816103	37.0	33.0	40.0	26.0	41.0
38	34.901295023680994	37.0	33.0	40.0	26.0	41.0
39	34.86941231959594	37.0	33.0	40.0	25.0	41.0
40	34.68832953679492	37.0	33.0	40.0	24.0	41.0
41	34.62907020651069	37.0	33.0	40.0	24.0	41.0
42	34.605497880741666	37.0	33.0	40.0	24.0	41.0
43	34.578668704880435	37.0	33.0	40.0	24.0	41.0
44	34.449370432111365	37.0	33.0	40.0	24.0	41.0
45	34.32070499184304	36.0	33.0	40.0	24.0	41.0
46	34.276406716791016	36.0	33.0	40.0	24.0	41.0
47	34.224970584246854	36.0	33.0	40.0	24.0	41.0
48	34.149491839154614	36.0	33.0	40.0	23.0	41.0
49	34.01611531249187	36.0	33.0	40.0	24.0	41.0
50	33.891106694372496	36.0	33.0	40.0	23.0	41.0
51	33.54990952686867	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	17.0
14	34.0
15	94.0
16	264.0
17	621.0
18	1308.0
19	2496.0
20	4256.0
21	6697.0
22	9854.0
23	14058.0
24	19820.0
25	26579.0
26	34394.0
27	41031.0
28	48755.0
29	58744.0
30	70769.0
31	85717.0
32	105702.0
33	133313.0
34	197602.0
35	223327.0
36	185015.0
37	229090.0
38	311329.0
39	379209.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.310662314459314	24.282047875194106	29.43109453997049	11.976195270376087
2	30.261493146825813	26.477373955152906	29.23252393782535	14.028608960195932
3	30.10862711417878	26.29304893311107	29.465840970927605	14.132482981782552
4	25.998959890016813	29.39936159711217	28.883552884204892	15.718125628666124
5	23.558308483471247	33.43177394456004	28.157302252399717	14.852615319568999
6	21.61794176023319	42.475708318094675	25.528581793144134	10.377768128528007
7	90.94588368940435	3.3181700091272166	4.361521774962754	1.3744245265056825
8	91.61675006266685	2.556031473600246	3.999172660540154	1.828045803192745
9	86.97154947622381	4.852446381599824	5.575683525306223	2.6003206168701456
10	41.45208120163294	31.21284220782749	13.840950837681204	13.494125752858363
11	34.92121326683569	26.743291450414652	22.618693762490565	15.716801520259088
12	33.02225917550052	23.91079527320694	25.414023586480035	17.652921964812503
13	25.18764670778708	30.00068944954987	26.51864961032403	18.29301423233902
14	19.637897443694573	33.108737152153296	28.217845967838777	19.035519436313354
15	18.965569985293264	26.899673219176922	36.84066820902467	17.294088586505143
16	22.387933628381212	24.961224170183545	34.69369491537806	17.957147286057186
17	22.55239702431748	24.67649520375974	27.519903860597868	25.251203911324914
18	24.091513241312366	25.970742683273723	30.324639420168364	19.613104655245547
19	27.08107607094116	27.37498247839306	26.21917281578045	19.324768634885324
20	28.230219532607997	27.830932359520087	25.538626753473388	18.40022135439853
21	26.093245540380057	26.790594081783325	28.58690694211774	18.52925343571887
22	24.605175985422022	24.733979772189432	27.838740033230554	22.822104209157988
23	22.22452038738846	28.48102392846527	27.503649288428704	21.79080639571756
24	22.233195580400093	26.77045850221423	32.10341925883717	18.89292665854851
25	21.887374991610177	27.545335873795118	29.64633064448009	20.920958490114618
26	20.77274966634751	31.539486054170645	26.161733906261343	21.5260303732205
27	20.427842255769345	30.264643611656357	29.137827357266822	20.16968677530748
28	18.342051902310022	29.208279056303372	31.86037687777977	20.589292163606835
29	19.886190599469536	27.171982961920925	32.01022942232803	20.93159701628151
30	21.620589977047267	27.44159882894026	30.840950381092096	20.096860812920376
31	25.330330803373098	27.300512795224808	26.6450334748303	20.7241229265718
32	26.19931118967488	27.243941405006865	27.410779064293685	19.145968341024577
33	25.538170164367514	27.822165848687284	26.02763368586578	20.61203030107942
34	21.176739707225934	28.678133445471616	28.6961230562431	21.449003791059347
35	20.84543865200285	27.645191911250034	29.805862878072904	21.703506558674214
36	25.87102362255057	26.96971398801819	27.198784742435798	19.96047764699544
37	21.57497672537033	30.012560766302627	27.826412127371924	20.586050380955122
38	21.93915219621643	30.450703672300524	25.77418107319443	21.83596305828862
39	21.248698150311874	28.852778778468902	28.025393659712357	21.873129411506866
40	23.876962020461583	26.507463177230083	27.29010256361085	22.32547223869748
41	19.72720627280377	26.42061992929261	28.7129711942499	25.139202603653715
42	22.22753387548724	26.720188041657362	27.381009454590615	23.671268628264784
43	21.822950268771176	27.30553527538944	27.630626718772618	23.24088773706677
44	20.854022527193305	28.18369310271931	28.16319225186551	22.79909211822188
45	20.33771156626957	30.277747718994974	26.55179797941057	22.832742735324885
46	22.137174891434526	28.982952332553936	26.852644541271776	22.027228234739766
47	20.86237810783082	27.708520820234934	28.516409584170603	22.912691487763638
48	21.903720881600506	26.797671212924396	29.39424779912636	21.904360106348737
49	22.015219941255246	25.540453109896887	30.144423700079404	22.299903248768466
50	20.563677514767235	29.237044169973515	28.559100665569943	21.640177649689313
51	20.18849824646954	29.553186466881538	26.855977641744666	23.40233764490426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	852.0
1	1474.5
2	2097.0
3	5438.5
4	8780.0
5	7189.0
6	5598.0
7	5509.0
8	5420.0
9	5798.5
10	6177.0
11	6473.5
12	6770.0
13	6946.0
14	7122.0
15	7043.0
16	6964.0
17	7161.5
18	7359.0
19	7651.5
20	7944.0
21	9208.5
22	10473.0
23	11643.0
24	12813.0
25	15646.5
26	21050.0
27	23620.0
28	26081.0
29	28542.0
30	35873.0
31	43204.0
32	50631.0
33	58058.0
34	62573.0
35	67088.0
36	75039.0
37	82990.0
38	92817.5
39	102645.0
40	118308.0
41	133971.0
42	151415.5
43	168860.0
44	174744.0
45	180628.0
46	179598.5
47	178569.0
48	175547.0
49	172525.0
50	166148.5
51	159772.0
52	150358.5
53	140945.0
54	129578.5
55	118212.0
56	111025.5
57	103839.0
58	95325.5
59	86812.0
60	78578.0
61	70344.0
62	62571.0
63	54798.0
64	46455.5
65	38113.0
66	31911.5
67	25710.0
68	21152.5
69	16595.0
70	13428.5
71	10262.0
72	8514.5
73	6767.0
74	5637.5
75	3695.0
76	2882.0
77	2263.0
78	1644.0
79	1242.0
80	840.0
81	586.0
82	332.0
83	219.5
84	107.0
85	84.5
86	62.0
87	43.5
88	25.0
89	17.5
90	10.0
91	8.5
92	7.0
93	7.0
94	7.0
95	7.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2190153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.978450364976936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81550036040917	15.147745119458245
2	7.239782371596487	2.747997007851579
3	2.314965725115653	1.3180338633219082
4	1.1932316487669121	0.9058275048016975
5	0.6938204710408743	0.6583818685927075
6	0.5193412116039403	0.591377484414742
7	0.38176672394097344	0.5071738574919532
8	0.287149688869595	0.43597248940241445
9	0.2539187101560648	0.4337085273692257
>10	3.862244440539945	19.33939404344001
>50	2.5029133989878267	34.71514087825353
>100	0.9314237572992706	22.41787904779959
>500	0.0034488052141362345	0.44456598090662675
>1k	4.926864591623192E-4	0.33680232689571343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4195	0.19153912991466807	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3009	0.1373876619578632	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02292077311493763	0.0
2	0.0	0.0	0.0	0.102504254268994	0.0
3	0.0	0.0	0.0	0.15072006384942058	0.0
4	0.0	0.0	0.0	0.30618865439994375	0.0
5	0.0	0.0	0.0	0.553385996320805	0.0
6	0.0	0.0	0.0	0.8640948828689137	0.0
7	0.0	0.0	0.0	1.0008433200785516	0.0
8	0.0	0.0	0.0	1.3483532885602056	0.0
9	0.0	0.0	0.0	1.453597077464451	0.0
10	0.0	0.0	0.0	1.675042793813948	0.0
11	0.0	0.0	0.0	1.9932397416984111	0.0
12	0.0	0.0	0.0	2.2330403401040932	0.0
13	0.0	0.0	0.0	2.3359098656577872	0.0
14	0.0	0.0	0.0	2.381523117334725	0.0
15	0.0	0.0	0.0	2.455764505950041	0.0
16	0.0	0.0	0.0	2.6121462747123148	0.0
17	0.0	0.0	0.0	2.784326026537872	0.0
18	0.0	0.0	0.0	3.001525464202729	0.0
19	0.0	0.0	0.0	3.1192797946079565	0.0
20	0.0	0.0	0.0	3.24817489919654	0.0
21	0.0	0.0	0.0	3.422226666356186	0.0
22	0.0	0.0	0.0	3.608378044821526	0.0
23	0.0	0.0	0.0	3.8036155464937838	0.0
24	0.0	0.0	0.0	3.95127646333384	0.0
25	0.0	0.0	0.0	4.087842264901128	0.0
26	0.0	0.0	0.0	4.2166917105791235	0.0
27	0.0	0.0	0.0	4.361704410605104	0.0
28	0.0	0.0	0.0	4.501740289377044	0.0
29	0.0	0.0	0.0	4.6629619026615945	0.0
30	0.0	0.0	0.0	4.8629022721243675	0.0
31	0.0	0.0	0.0	5.044076829335667	0.0
32	0.0	0.0	0.0	5.217398053925913	0.0
33	0.0	0.0	0.0	5.391997728012609	0.0
34	0.0	0.0	0.0	5.570843680783946	0.0
35	0.0	0.0	0.0	5.776034824964284	0.0
36	0.0	0.0	0.0	5.9648344202437	0.0
37	0.0	0.0	0.0	6.162080913981809	0.0
38	0.0	0.0	0.0	6.378047561060803	0.0
39	0.0	0.0	0.0	6.717430243457877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACG	265	0.0	42.45283	26
CGCGTAA	65	0.0	41.538464	31
CGGGTAT	175	0.0	41.142857	6
CGGTCTA	275	0.0	40.909092	31
TCGCGCG	50	1.0822987E-9	40.5	1
ACGGGTA	275	0.0	40.090908	5
GGGCGAT	3455	0.0	39.725037	7
ACAACGA	485	0.0	39.43299	13
AGTACGG	195	0.0	39.23077	2
CGACGGT	290	0.0	38.793106	28
ACGGGAT	700	0.0	38.571426	5
TCGATCA	170	0.0	38.38235	17
TCACGAC	300	0.0	38.25	25
GCGATAC	65	9.094947E-12	38.076927	9
GCGATCT	195	0.0	38.076923	9
TGGGCGA	1125	0.0	38.0	6
AGGGCGA	1815	0.0	37.933884	6
TATAGCG	95	0.0	37.894737	1
GGCACCG	775	0.0	37.741936	8
TACGGGA	830	0.0	37.68072	4
>>END_MODULE