FastQCFastQC Report
Thu 26 May 2016
SRR1547979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547979_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2636657
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262430.9953133835762483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149800.042.4165531
TACGGGA10550.040.308064
AGGGCGA22450.038.485526
GGGCGAT39900.038.4586457
ACGGGAT13650.038.4065935
GACCGAT13300.037.8947339
TTAGGGA42650.037.6143044
TAGGGAC32950.037.5569045
TAGGGAT41400.037.3369525
AGGGATT46600.037.0815476
TAAGGGA38850.037.065644
GTAGGGA42400.037.0400964
AAGGGAT46050.036.986975
TCGATAG1400.036.9642831
CTATGCG552.752131E-936.818181
TGTAGCG1850.036.486491
CGGTTTT13400.036.436571
TGAGGGA48850.036.340844
GAGGGAT44350.036.324695
AAGGGAC36100.036.0872575
Produced by FastQC (version 0.11.5)