##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547979_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2636657
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86234197318802	31.0	31.0	31.0	30.0	33.0
2	31.17423464637228	31.0	31.0	33.0	30.0	34.0
3	31.202876976413695	31.0	31.0	34.0	28.0	34.0
4	35.18637615738414	35.0	35.0	37.0	33.0	37.0
5	35.082466547601754	35.0	35.0	37.0	32.0	37.0
6	35.141041857169895	37.0	35.0	37.0	32.0	37.0
7	35.52736021408928	35.0	35.0	37.0	35.0	37.0
8	35.62064424762114	37.0	35.0	37.0	35.0	37.0
9	37.452678903626826	39.0	37.0	39.0	35.0	39.0
10	36.61379011376906	39.0	37.0	39.0	32.0	39.0
11	36.19450956267728	38.0	35.0	39.0	31.0	39.0
12	35.91482130591882	37.0	35.0	39.0	31.0	39.0
13	35.82520365751025	38.0	35.0	39.0	30.0	39.0
14	36.83388434673149	39.0	35.0	40.0	31.0	41.0
15	36.96763325680966	39.0	35.0	41.0	31.0	41.0
16	37.03483729586366	39.0	35.0	41.0	31.0	41.0
17	36.96596258064663	39.0	35.0	41.0	31.0	41.0
18	36.830093940925956	38.0	35.0	40.0	31.0	41.0
19	36.73436855836766	38.0	35.0	40.0	31.0	41.0
20	36.552792797849705	38.0	35.0	40.0	30.0	41.0
21	36.447166620459164	38.0	35.0	40.0	30.0	41.0
22	36.35669372239165	38.0	35.0	40.0	30.0	41.0
23	36.26596861101008	38.0	35.0	40.0	30.0	41.0
24	36.13509303637144	38.0	34.0	40.0	30.0	41.0
25	35.963916049755426	38.0	34.0	40.0	30.0	41.0
26	35.74537150641893	38.0	34.0	40.0	29.0	41.0
27	35.81505027009581	38.0	34.0	40.0	29.0	41.0
28	35.82017228634593	38.0	34.0	40.0	29.0	41.0
29	35.77533596520139	38.0	34.0	40.0	29.0	41.0
30	35.66637753792018	38.0	34.0	40.0	29.0	41.0
31	35.559079925830325	38.0	34.0	40.0	28.0	41.0
32	35.38074349450839	38.0	34.0	40.0	27.0	41.0
33	35.15687933622007	38.0	34.0	40.0	26.0	41.0
34	34.87686566739625	38.0	33.0	40.0	24.0	41.0
35	34.66258978699163	38.0	33.0	40.0	24.0	41.0
36	34.56686364589706	38.0	33.0	40.0	23.0	41.0
37	34.50898163849147	38.0	33.0	40.0	23.0	41.0
38	34.406847762147294	38.0	33.0	40.0	23.0	41.0
39	34.35736009651615	38.0	33.0	40.0	23.0	41.0
40	34.21509206544499	37.0	33.0	40.0	22.0	41.0
41	34.1126202611868	37.0	33.0	40.0	22.0	41.0
42	34.068138935022645	37.0	33.0	40.0	22.0	41.0
43	34.0275932743622	37.0	33.0	40.0	22.0	41.0
44	33.87702192587052	37.0	33.0	40.0	21.0	41.0
45	33.76014058711467	37.0	33.0	40.0	21.0	41.0
46	33.709756710865314	37.0	32.0	40.0	21.0	41.0
47	33.66346134518066	37.0	32.0	40.0	20.0	41.0
48	33.56221457701931	36.0	32.0	40.0	20.0	41.0
49	33.42173441596689	36.0	32.0	40.0	20.0	41.0
50	33.3349722773952	36.0	32.0	40.0	20.0	41.0
51	33.01448538812595	35.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	16.0
14	44.0
15	132.0
16	304.0
17	807.0
18	1720.0
19	3267.0
20	5455.0
21	8811.0
22	13224.0
23	19142.0
24	28492.0
25	41283.0
26	54474.0
27	61758.0
28	65823.0
29	71936.0
30	83340.0
31	98664.0
32	118780.0
33	146875.0
34	202719.0
35	231260.0
36	236637.0
37	288797.0
38	388631.0
39	464230.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.53939135807198	24.037370048512187	28.928184439614252	16.495054153801576
2	32.71206683311481	25.34296269859902	28.811900827449303	13.133069640836863
3	27.904729359943293	25.324113071969546	33.446026540426	13.325131027661163
4	24.843125214997627	28.000684199727154	33.14985604877692	14.006334536498299
5	22.398590336171903	32.08426427859217	31.893264842563894	13.62388054267203
6	20.99518443240816	40.95079488913424	28.229838010784107	9.824182667673497
7	88.18977970968541	2.4942948589824163	8.145276385969051	1.1706490453631246
8	88.83119799048568	2.0587433253547958	7.865073083074514	1.2449856010850102
9	84.07961293410557	4.932837301173418	9.036253103835652	1.9512966608853561
10	39.1699413310112	36.79962922746493	14.498131535501205	9.532297906022665
11	26.38663277020864	25.60905722663206	29.68819986824225	18.316110134917054
12	27.076256031785707	22.853067350057287	30.966940333915254	19.10373628424175
13	24.583516172183185	24.445386715071397	31.3047923943084	19.666304718437022
14	19.542852938398887	27.645992633854156	32.29767846177944	20.513475965967512
15	19.23003257534067	27.497698790551823	34.83831988764561	18.433948746461905
16	23.575193891355607	26.114052756957012	32.28155956576832	18.029193785919063
17	23.17233527151996	26.16787090622709	30.224826361563146	20.434967460689805
18	22.639842800940738	26.24474855849661	32.50176264868733	18.61364599187532
19	24.66896528444921	27.576169369015386	29.062179873984366	18.692685472551037
20	25.36799439593394	28.075096609077328	29.12623826307328	17.430670731915452
21	24.876538738258333	26.2555577005276	31.24483010114702	17.623073460067047
22	24.25120142665504	23.761300768359327	31.50182219378554	20.48567561120009
23	21.97206538431051	26.678024483275603	31.10025308563078	20.249657046783103
24	21.325716617671546	27.07022566833684	32.61679467598554	18.98726303800608
25	22.398173141216322	28.270381775103854	29.95691134645121	19.37453373722862
26	21.200520204182798	29.43697265135359	28.957767354646435	20.40473978981718
27	19.654206064725145	29.24828675098809	31.196738900812658	19.90076828347411
28	19.581955483781165	28.212846798047682	32.11210256017373	20.093095157997418
29	20.657294445200876	26.982311313151463	31.927892023877202	20.432502217770455
30	21.39227817649395	27.210061832085096	31.569142288890816	19.828517702530135
31	22.86330758987612	27.40034065864464	29.886481252586133	19.849870498893104
32	23.565332919678212	27.60385594334037	29.44869203692403	19.38211910005738
33	23.248454387506605	27.71869833656786	29.559779675551273	19.47306760037426
34	21.538220557319363	27.714222972498888	30.534915994003015	20.212640476178738
35	21.449585592665258	28.62025663558059	29.747669112819757	20.1824886589344
36	23.107177004820876	28.990043073482823	28.24007825060294	19.66270167109336
37	22.238918448626425	29.561107114046308	28.435932318841623	19.76404211848564
38	21.91126870123797	29.50433067327301	28.40263257602335	20.181768049465667
39	21.416665117988423	29.237363828514667	28.151026090993252	21.194944962503655
40	23.00769497132164	28.131759269408196	28.365198810463397	20.495346948806766
41	20.586029961424636	28.302202372170516	28.945630774120414	22.166136892284435
42	21.171582044991062	28.559080684366606	28.512999605181864	21.756337665460467
43	21.385640984018778	28.171506570630918	28.938841874388665	21.504010570961636
44	21.379231352428473	28.496122172887866	28.871787266982395	21.252859207701267
45	20.877876796261326	29.073406210970937	28.08522306845373	21.963493924314008
46	21.299016140514297	29.222420663742003	28.1900148559331	21.2885483398106
47	20.74145404578601	29.069310115043407	29.255834187002712	20.933401652167877
48	21.012516986471887	28.48967461448342	29.681448895324646	20.816359503720054
49	21.554984209170932	28.029205163963304	29.283293200442834	21.13251742642293
50	20.14505489337445	28.822709969480293	29.871424307371036	21.160810829774217
51	20.063436389336953	28.85532702964398	29.403862542606035	21.677374038413035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1044.0
1	1869.0
2	2694.0
3	23238.5
4	43783.0
5	32920.0
6	22057.0
7	20432.0
8	18807.0
9	19489.0
10	20171.0
11	20719.5
12	21268.0
13	21005.5
14	20743.0
15	19657.0
16	18571.0
17	17839.0
18	17107.0
19	16350.0
20	15593.0
21	15731.0
22	15869.0
23	17066.0
24	18263.0
25	21047.5
26	27185.0
27	30538.0
28	36184.0
29	41830.0
30	49496.0
31	57162.0
32	64417.5
33	71673.0
34	80831.5
35	89990.0
36	96156.5
37	102323.0
38	111039.0
39	119755.0
40	132177.0
41	144599.0
42	152714.5
43	160830.0
44	166357.0
45	171884.0
46	177668.5
47	183453.0
48	185948.0
49	188443.0
50	183165.0
51	177887.0
52	168315.5
53	158744.0
54	151119.5
55	143495.0
56	135066.0
57	126637.0
58	119535.0
59	112433.0
60	101043.5
61	89654.0
62	78402.5
63	67151.0
64	58346.0
65	49541.0
66	42024.0
67	34507.0
68	28219.5
69	21932.0
70	17869.5
71	13807.0
72	11178.0
73	8549.0
74	6594.0
75	3523.5
76	2408.0
77	1907.0
78	1406.0
79	1110.5
80	815.0
81	636.5
82	458.0
83	299.5
84	141.0
85	104.0
86	67.0
87	50.0
88	33.0
89	29.5
90	26.0
91	20.0
92	14.0
93	15.0
94	16.0
95	15.5
96	15.0
97	7.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2636657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.120338364385614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01829019385455	17.612258662646717
2	6.72765388574657	3.245465762453619
3	3.0301466501904626	2.192644874889107
4	1.8331607135354038	1.7686582674709004
5	1.3898363981913866	1.676166209775761
6	1.0580345112308822	1.5312090247251735
7	0.8491102815023577	1.433657910896091
8	0.7784778102214994	1.5021718553366827
9	0.7008772016114223	1.52148557292761
>10	9.945436622954077	55.55602905402497
>50	0.6395644459513395	9.368058418562942
>100	0.02682645289987208	1.080902497590504
>500	0.0019386240825950691	0.2874197857634907
>1k	4.846560206487669E-4	0.20126399043769372
>5k	0.0	0.0
>10k+	1.6155200688292232E-4	1.022608112498818
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26243	0.9953133835762483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016649871409136645	0.0
2	0.0	0.0	0.0	0.051428759979018884	0.0
3	0.0	0.0	0.0	0.08996240314913923	0.0
4	0.0	0.0	0.0	0.14419774737480073	0.0
5	0.0	0.0	0.0	0.28558891050295887	0.0
6	0.0	0.0	0.0	0.5704192847230414	0.0
7	0.0	0.0	0.0	0.753871284736695	0.0
8	0.0	0.0	0.0	1.0814831053110057	0.0
9	0.0	0.0	0.0	1.285718999475472	0.0
10	0.0	0.0	0.0	1.5314089014991332	0.0
11	0.0	0.0	0.0	1.7504362531796893	0.0
12	0.0	0.0	0.0	1.9124216763879411	0.0
13	0.0	0.0	0.0	2.019033950946217	0.0
14	0.0	0.0	0.0	2.097770017108786	0.0
15	0.0	0.0	0.0	2.1625110888522854	0.0
16	0.0	0.0	0.0	2.2489083714719054	0.0
17	0.0	0.0	0.0	2.3541552807210038	0.0
18	0.0	0.0	0.0	2.47745535350256	0.0
19	0.0	0.0	0.0	2.5713621453226567	0.0
20	0.0	0.0	0.0	2.665117229886178	0.0
21	0.0	0.0	0.0	2.779883769485375	0.0
22	0.0	0.0	0.0	2.9161168858899735	0.0
23	0.0	0.0	0.0	3.0562564641513856	0.0
24	0.0	0.0	0.0	3.1729193444577737	0.0
25	0.0	0.0	0.0	3.2807452770686516	0.0
26	0.0	0.0	0.0	3.3900503554311388	0.0
27	0.0	0.0	0.0	3.5159673783886185	0.0
28	0.0	0.0	0.0	3.6371056227639773	0.0
29	0.0	0.0	0.0	3.7816447114660723	0.0
30	0.0	0.0	0.0	3.9576251290933935	0.0
31	0.0	0.0	0.0	4.10952201973939	0.0
32	0.0	0.0	0.0	4.256450497732545	0.0
33	0.0	0.0	0.0	4.399624221125463	0.0
34	0.0	0.0	0.0	4.563923179996488	0.0
35	0.0	0.0	0.0	4.741724084702713	0.0
36	0.0	0.0	0.0	4.901244264991616	0.0
37	0.0	0.0	0.0	5.080903583590888	0.0
38	0.0	0.0	0.0	5.282674234836007	0.0
39	0.0	0.0	0.0	5.604331545589737	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14980	0.0	42.416553	1
TACGGGA	1055	0.0	40.30806	4
AGGGCGA	2245	0.0	38.48552	6
GGGCGAT	3990	0.0	38.458645	7
ACGGGAT	1365	0.0	38.406593	5
GACCGAT	1330	0.0	37.894733	9
TTAGGGA	4265	0.0	37.614304	4
TAGGGAC	3295	0.0	37.556904	5
TAGGGAT	4140	0.0	37.336952	5
AGGGATT	4660	0.0	37.081547	6
TAAGGGA	3885	0.0	37.06564	4
GTAGGGA	4240	0.0	37.040096	4
AAGGGAT	4605	0.0	36.98697	5
TCGATAG	140	0.0	36.964283	1
CTATGCG	55	2.752131E-9	36.81818	1
TGTAGCG	185	0.0	36.48649	1
CGGTTTT	1340	0.0	36.43657	1
TGAGGGA	4885	0.0	36.34084	4
GAGGGAT	4435	0.0	36.32469	5
AAGGGAC	3610	0.0	36.087257	5
>>END_MODULE