FastQCFastQC Report
Thu 26 May 2016
SRR1547978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547978_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2645618
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157380.5948704612684068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97250.041.784061
TACGGGA11800.040.233054
TAGGGAC32500.039.253855
ACGGGAC10150.038.7931025
GGGCGAT38650.037.6067287
AACGGGA12400.037.5604824
TAGGGAT46100.037.4349255
ACGGGAT14000.037.1255
TTAGGGA40150.036.7621424
GCGTAAG1900.036.7105261
CTAGGGA24350.036.6837774
TAAGGGA40200.036.5485084
TTGGGAC24000.036.3755
GTAGGGA40550.036.2885324
ATAGGGA41600.036.2379844
AAGGGAT47450.036.2276085
AGGGATC37300.036.1930276
AGGGCGA21450.036.188816
AGGGATT53650.035.899356
TAGGGCG8500.035.7352945
Produced by FastQC (version 0.11.5)