##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547978_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2645618
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.857216725921884	31.0	31.0	31.0	30.0	33.0
2	31.19950196891615	31.0	31.0	33.0	30.0	34.0
3	31.30449180493934	31.0	31.0	34.0	30.0	34.0
4	35.23012052382468	37.0	35.0	37.0	33.0	37.0
5	35.07489932408987	35.0	35.0	37.0	32.0	37.0
6	35.1196438790483	37.0	35.0	37.0	32.0	37.0
7	35.52833591244087	35.0	35.0	37.0	35.0	37.0
8	35.61886296509927	37.0	35.0	37.0	35.0	37.0
9	37.42643571369714	39.0	37.0	39.0	35.0	39.0
10	36.56121480878948	38.0	35.0	39.0	32.0	39.0
11	36.1391194798342	38.0	35.0	39.0	31.0	39.0
12	35.843771096205124	37.0	35.0	39.0	30.0	39.0
13	35.72798718484679	37.0	35.0	39.0	30.0	39.0
14	36.75763167622839	39.0	35.0	40.0	31.0	41.0
15	36.89673301285371	39.0	35.0	40.0	31.0	41.0
16	36.967965140848	39.0	35.0	41.0	31.0	41.0
17	36.881012300339655	38.0	35.0	40.0	31.0	41.0
18	36.77442472798416	38.0	35.0	40.0	31.0	41.0
19	36.70992562040325	38.0	35.0	40.0	31.0	41.0
20	36.58172192659711	38.0	35.0	40.0	30.0	41.0
21	36.470464745855224	38.0	35.0	40.0	30.0	41.0
22	36.35767144009453	38.0	35.0	40.0	30.0	41.0
23	36.25233159133329	38.0	34.0	40.0	30.0	41.0
24	36.14765812751501	38.0	34.0	40.0	30.0	41.0
25	35.98532101006268	38.0	34.0	40.0	30.0	41.0
26	35.76818119622712	38.0	34.0	40.0	29.0	41.0
27	35.856357191401024	38.0	34.0	40.0	29.0	41.0
28	35.85760453701177	38.0	34.0	40.0	29.0	41.0
29	35.83356289532351	38.0	34.0	40.0	29.0	41.0
30	35.729962148730465	38.0	34.0	40.0	29.0	41.0
31	35.621254466820226	38.0	34.0	40.0	28.0	41.0
32	35.48795555518597	38.0	34.0	40.0	27.0	41.0
33	35.30343042721965	38.0	34.0	40.0	27.0	41.0
34	35.09690552453151	38.0	34.0	40.0	25.0	41.0
35	34.913980778782125	38.0	33.0	40.0	25.0	41.0
36	34.82555002271681	38.0	33.0	40.0	24.0	41.0
37	34.780447517366454	38.0	33.0	40.0	24.0	41.0
38	34.71414769630385	38.0	33.0	40.0	24.0	41.0
39	34.65295216467381	38.0	33.0	40.0	24.0	41.0
40	34.48940927979776	38.0	33.0	40.0	23.0	41.0
41	34.40169253459872	37.0	33.0	40.0	23.0	41.0
42	34.35688447840921	37.0	33.0	40.0	23.0	41.0
43	34.32353763846481	37.0	33.0	40.0	23.0	41.0
44	34.15663637002772	37.0	33.0	40.0	23.0	41.0
45	34.023666682038	37.0	33.0	40.0	23.0	41.0
46	33.94181737499518	37.0	33.0	40.0	23.0	41.0
47	33.883840751007895	37.0	33.0	40.0	22.0	41.0
48	33.77052053622254	36.0	32.0	40.0	22.0	41.0
49	33.62021728004572	36.0	32.0	40.0	21.0	41.0
50	33.5133129575018	36.0	32.0	40.0	21.0	41.0
51	33.1814033620878	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	5.0
14	35.0
15	96.0
16	284.0
17	671.0
18	1452.0
19	2903.0
20	5081.0
21	8370.0
22	12397.0
23	18390.0
24	26360.0
25	37197.0
26	48499.0
27	56306.0
28	62695.0
29	72301.0
30	84848.0
31	101243.0
32	121357.0
33	151375.0
34	207962.0
35	238791.0
36	232052.0
37	287642.0
38	392314.0
39	474943.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.45409427967303	25.364357212568102	29.633529859564007	14.548018648194864
2	31.369759353013173	26.440060507601626	28.853069490757928	13.337110648627275
3	28.06973644721196	26.301416153050063	32.062867730715475	13.565979669022513
4	25.238110717420277	28.744890607789937	31.419312992276282	14.5976856825135
5	23.222702597275948	32.46976698828024	30.068664485953754	14.238865928490055
6	22.087277906334172	41.489776679777655	26.162620605091135	10.260324808797037
7	89.82343633888188	2.6497022623825512	6.335948727291695	1.1909126714438742
8	90.69434816364267	1.9220083927460427	5.866039617208531	1.5176038264027536
9	85.47507614477978	4.893866007866593	7.436334346077174	2.1947235012764503
10	39.06285790314399	37.01142795369551	13.109035393620697	10.816678749539806
11	26.906718959426495	25.6668952207008	29.323772366229743	18.102613453642967
12	27.56577102212035	23.1032220071076	30.024440414300173	19.30656655647187
13	23.71563090363008	26.070997400229363	30.75092473667778	19.462446959462778
14	19.11542029121362	29.290850001776526	30.833362942042275	20.760366764967582
15	18.583748674222807	27.690240994731663	35.479725342056184	18.246284988989338
16	23.005740057710526	25.576179176283198	33.72444547927932	17.69363528672696
17	22.28103225786943	25.859742411791874	29.76726042837628	22.091964901962417
18	22.696549539653873	25.957791336466563	31.590161542596096	19.755497581283464
19	24.247982890954024	27.57136517819277	28.64343983144959	19.537212099403618
20	25.81294805221313	27.476680306831902	28.063310727399042	18.647060913555926
21	24.930318738381732	26.646477307003508	30.294471839849894	18.128732114764865
22	24.12581861780499	24.07524442304218	30.418223643776237	21.380713315376596
23	21.4307205348618	27.355649984238088	30.180849994216853	21.03277948668326
24	21.268943588983745	26.533044453129666	32.79381225861028	19.40419969927631
25	21.957062584243076	28.204563168227615	29.43198904754957	20.40638519997974
26	21.156115508739358	30.19101775086199	28.11898769966034	20.533879040738306
27	20.29446428017953	29.172994740737323	30.297155522830582	20.235385456252565
28	19.56008010226722	28.9048154344278	31.903698871114422	19.63140559219056
29	20.82670287244795	26.719919504629917	31.859701589571888	20.593676033350242
30	22.07355710461601	27.035535742499484	31.061249205289656	19.829657947594853
31	23.40995563229461	27.24558118367807	29.419137607923744	19.92532557610358
32	24.04247325199632	27.119485881937607	29.437885590436714	19.40015527562936
33	23.228258954996527	27.42716446592063	29.316250494213453	20.0283260848694
34	21.68551922461973	27.490287713494542	30.46550938192891	20.35868367995682
35	21.195274601246286	27.266521470597798	30.413612244851674	21.124591683304242
36	23.260349755709253	27.825672489376775	28.884744509600402	20.02923324531357
37	21.394093931928193	28.651377485336127	29.269985311560475	20.684543271175205
38	21.649535193667415	28.567616337657213	28.683959664622783	21.09888880405259
39	21.048276810937935	28.451537599154523	28.62370909178876	21.876476498118777
40	23.00264059285959	27.227211184683505	28.605679277960768	21.164468944496146
41	20.171695233401042	27.265387520042577	29.49549027864189	23.067426967914493
42	21.487418062622798	27.812405267880695	28.395331449967454	22.30484521952905
43	21.37916358295113	27.51243754767317	29.22787794760997	21.88052092176573
44	21.40399710011045	28.778720132687337	28.6725445623669	21.144738204835317
45	21.006509632154	28.636295942951705	27.91453641455418	22.442658010340118
46	21.61759558636205	28.82226383400778	28.1539889734648	21.406151606165363
47	21.63517181996796	27.737035354310414	29.142415874098226	21.4853769516234
48	21.314226014488863	27.090721336186856	30.14550853524583	21.44954411407845
49	21.48114353621725	26.82027412876689	29.80165692855129	21.896925406464575
50	20.30043641977035	28.288399912610213	29.98157708331286	21.429586584306577
51	20.309243435749227	28.142876258023648	29.1763209956993	22.371559310527825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	959.0
1	1575.5
2	2192.0
3	14885.0
4	27578.0
5	20948.0
6	14318.0
7	13403.5
8	12489.0
9	13065.0
10	13641.0
11	13853.5
12	14066.0
13	14148.5
14	14231.0
15	13946.5
16	13662.0
17	13285.5
18	12909.0
19	12828.0
20	12747.0
21	13370.5
22	13994.0
23	15751.5
24	17509.0
25	20161.0
26	27400.0
27	31987.0
28	38034.0
29	44081.0
30	51514.5
31	58948.0
32	68155.5
33	77363.0
34	85568.5
35	93774.0
36	102282.5
37	110791.0
38	120223.5
39	129656.0
40	141222.5
41	152789.0
42	165150.0
43	177511.0
44	183955.0
45	190399.0
46	198298.5
47	206198.0
48	203624.0
49	201050.0
50	193122.0
51	185194.0
52	173938.5
53	162683.0
54	150225.5
55	137768.0
56	128631.0
57	119494.0
58	110732.5
59	101971.0
60	92834.5
61	83698.0
62	74021.0
63	64344.0
64	54771.0
65	45198.0
66	36944.0
67	28690.0
68	23333.0
69	17976.0
70	14961.5
71	11947.0
72	9934.5
73	7922.0
74	6611.0
75	4088.0
76	2876.0
77	2187.5
78	1499.0
79	1143.5
80	788.0
81	557.0
82	326.0
83	230.0
84	134.0
85	116.0
86	98.0
87	63.0
88	28.0
89	22.0
90	16.0
91	12.0
92	8.0
93	6.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2645618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.073567998620202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55356914812336	17.66363983276298
2	6.696803259798126	3.09038290776667
3	2.5796923720202174	1.7856812208399102
4	1.5286991012052424	1.41090170644355
5	0.9635026711536882	1.111572219985841
6	0.7483766607129883	1.0360631861724874
7	0.6216156036899062	1.0040022930519679
8	0.5126836390899768	0.9463552646658112
9	0.450477813392032	0.9354717413253715
>10	7.750384918538744	45.62183653367043
>50	1.5459237035461237	22.70138087918997
>100	0.045748512862729615	1.5958141738173806
>500	0.0015135575201132396	0.23181225742174527
>1k	8.408652889517998E-4	0.2543959088813127
>5k	0.0	0.0
>10k+	1.6817305779035996E-4	0.6106898740045911
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15738	0.5948704612684068	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018521192401926505	0.0
2	0.0	0.0	0.0	0.09177439826913787	0.0
3	0.0	0.0	0.0	0.18215025752017108	0.0
4	0.0	0.0	0.0	0.3003457037259347	0.0
5	0.0	0.0	0.0	0.5696589605906824	0.0
6	0.0	0.0	0.0	1.1572721383056812	0.0
7	0.0	0.0	0.0	1.4170224121547403	0.0
8	0.0	0.0	0.0	1.903638393751479	0.0
9	0.0	0.0	0.0	2.1787347984478487	0.0
10	0.0	0.0	0.0	2.531431219473106	0.0
11	0.0	0.0	0.0	2.897924038920207	0.0
12	0.0	0.0	0.0	3.17491036120861	0.0
13	0.0	0.0	0.0	3.3399001669931185	0.0
14	0.0	0.0	0.0	3.4432030625736596	0.0
15	0.0	0.0	0.0	3.5404204235078534	0.0
16	0.0	0.0	0.0	3.6860952715017814	0.0
17	0.0	0.0	0.0	3.852332422897032	0.0
18	0.0	0.0	0.0	4.0443102518957765	0.0
19	0.0	0.0	0.0	4.188170779001353	0.0
20	0.0	0.0	0.0	4.330897355551708	0.0
21	0.0	0.0	0.0	4.497399095409844	0.0
22	0.0	0.0	0.0	4.681136883707323	0.0
23	0.0	0.0	0.0	4.872623334132139	0.0
24	0.0	0.0	0.0	5.029448695919063	0.0
25	0.0	0.0	0.0	5.166127536174913	0.0
26	0.0	0.0	0.0	5.3113488039467525	0.0
27	0.0	0.0	0.0	5.464583322308814	0.0
28	0.0	0.0	0.0	5.617439857152469	0.0
29	0.0	0.0	0.0	5.780766535455988	0.0
30	0.0	0.0	0.0	5.977355763379293	0.0
31	0.0	0.0	0.0	6.160488778047322	0.0
32	0.0	0.0	0.0	6.335910928939854	0.0
33	0.0	0.0	0.0	6.508913985314584	0.0
34	0.0	0.0	0.0	6.6900436873350575	0.0
35	0.0	0.0	0.0	6.880660775667538	0.0
36	0.0	0.0	0.0	7.07025730850032	0.0
37	0.0	0.0	0.0	7.259060075944449	0.0
38	0.0	0.0	0.0	7.4588243654223705	0.0
39	0.0	0.0	0.0	7.7257563261211555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9725	0.0	41.78406	1
TACGGGA	1180	0.0	40.23305	4
TAGGGAC	3250	0.0	39.25385	5
ACGGGAC	1015	0.0	38.793102	5
GGGCGAT	3865	0.0	37.606728	7
AACGGGA	1240	0.0	37.560482	4
TAGGGAT	4610	0.0	37.434925	5
ACGGGAT	1400	0.0	37.125	5
TTAGGGA	4015	0.0	36.762142	4
GCGTAAG	190	0.0	36.710526	1
CTAGGGA	2435	0.0	36.683777	4
TAAGGGA	4020	0.0	36.548508	4
TTGGGAC	2400	0.0	36.375	5
GTAGGGA	4055	0.0	36.288532	4
ATAGGGA	4160	0.0	36.237984	4
AAGGGAT	4745	0.0	36.227608	5
AGGGATC	3730	0.0	36.193027	6
AGGGCGA	2145	0.0	36.18881	6
AGGGATT	5365	0.0	35.89935	6
TAGGGCG	850	0.0	35.735294	5
>>END_MODULE