FastQCFastQC Report
Thu 26 May 2016
SRR1547977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547977_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2629652
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83390.3171142037045206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT46500.039.096777
CGTAAGG4550.039.0659332
ACGGGAT13050.038.9655155
ATAACGG2100.038.571432
TACGGGA9600.038.4374964
CGTTTTT58100.038.1067121
TCGATAG1550.037.7419361
CGTTAGG2950.037.3728832
AGGGCGA26450.037.3440446
TAGGGAT41900.037.3215
TAAGGGA36700.036.9686624
GGCGATT12350.036.801628
AGGGATT44150.036.6930926
ACGTAAG1350.036.6666641
CGAATAT3450.036.5217414
CACGACC7450.036.2416127
ATAGGGA41400.036.1956524
TTAGGGA35350.036.0254634
AGGGATC25300.036.0177846
GACCGAT14500.036.09
Produced by FastQC (version 0.11.5)