##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547977_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2629652
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88076292984775	31.0	31.0	31.0	30.0	33.0
2	31.23378188444707	31.0	31.0	33.0	30.0	34.0
3	31.42926744679524	31.0	31.0	34.0	30.0	34.0
4	35.318164913075954	37.0	35.0	37.0	33.0	37.0
5	35.15331306195649	37.0	35.0	37.0	32.0	37.0
6	35.1862748378873	37.0	35.0	37.0	32.0	37.0
7	35.497550626470726	35.0	35.0	37.0	35.0	37.0
8	35.558430164903946	37.0	35.0	37.0	35.0	37.0
9	37.371436220458065	39.0	37.0	39.0	35.0	39.0
10	36.531036806391114	38.0	35.0	39.0	32.0	39.0
11	36.18668705973262	38.0	35.0	39.0	31.0	39.0
12	35.745845457878076	37.0	35.0	39.0	30.0	39.0
13	35.56931563568107	37.0	35.0	39.0	30.0	39.0
14	36.56383810481387	38.0	35.0	40.0	30.0	41.0
15	36.723051567279626	38.0	35.0	40.0	31.0	41.0
16	36.815714398711314	38.0	35.0	40.0	31.0	41.0
17	36.739638552934004	38.0	35.0	40.0	31.0	41.0
18	36.6617613281149	38.0	35.0	40.0	31.0	41.0
19	36.61753266211651	38.0	35.0	40.0	30.0	41.0
20	36.50661722539712	38.0	35.0	40.0	30.0	41.0
21	36.395351552220596	38.0	35.0	40.0	30.0	41.0
22	36.27844749039036	38.0	34.0	40.0	30.0	41.0
23	36.165708618478796	38.0	34.0	40.0	30.0	41.0
24	36.05944208587296	38.0	34.0	40.0	30.0	41.0
25	35.88173910464198	38.0	34.0	40.0	29.0	41.0
26	35.65377129749488	38.0	34.0	40.0	29.0	41.0
27	35.75244823269391	38.0	34.0	40.0	29.0	41.0
28	35.76583821737629	38.0	34.0	40.0	29.0	41.0
29	35.74059989686848	38.0	34.0	40.0	29.0	41.0
30	35.64867708731041	38.0	34.0	40.0	29.0	41.0
31	35.57761102990053	38.0	34.0	40.0	28.0	41.0
32	35.465742995651134	38.0	34.0	40.0	27.0	41.0
33	35.34025376741865	38.0	34.0	40.0	27.0	41.0
34	35.169113631765725	38.0	34.0	40.0	26.0	41.0
35	35.038412687306156	38.0	34.0	40.0	25.0	41.0
36	34.97459017390894	38.0	33.0	40.0	25.0	41.0
37	34.93141031588971	38.0	33.0	40.0	25.0	41.0
38	34.84382876517501	37.0	33.0	40.0	25.0	41.0
39	34.79157166043264	37.0	33.0	40.0	25.0	41.0
40	34.6069095834734	37.0	33.0	40.0	24.0	41.0
41	34.52257827271441	37.0	33.0	40.0	24.0	41.0
42	34.48664880372004	37.0	33.0	40.0	24.0	41.0
43	34.42383554934265	37.0	33.0	40.0	24.0	41.0
44	34.26829443591776	37.0	33.0	40.0	23.0	41.0
45	34.13337924561881	36.0	33.0	40.0	23.0	41.0
46	34.056381604866345	36.0	33.0	40.0	23.0	41.0
47	34.001815449344626	36.0	33.0	40.0	23.0	41.0
48	33.90607312298357	36.0	33.0	40.0	23.0	41.0
49	33.7517800834483	36.0	32.0	40.0	23.0	41.0
50	33.65387093045011	36.0	32.0	40.0	23.0	41.0
51	33.305214910566114	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	13.0
14	41.0
15	138.0
16	364.0
17	783.0
18	1645.0
19	3077.0
20	5133.0
21	8191.0
22	12091.0
23	17470.0
24	24399.0
25	33242.0
26	43202.0
27	51313.0
28	59684.0
29	70314.0
30	84641.0
31	102403.0
32	125121.0
33	156747.0
34	223252.0
35	246483.0
36	236599.0
37	290129.0
38	386986.0
39	446124.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.144061647700916	24.943148370963154	27.712868470809067	13.199921510526869
2	30.83639964527626	26.046336169196532	28.931851058619163	14.185413126908047
3	29.713171172459322	26.26035688372454	29.69468963954166	14.331782304274482
4	26.57093029800141	28.894735881401797	28.968243706771844	15.566090113824949
5	23.703440607350327	33.05619907120791	28.015798288138505	15.224562033303265
6	22.773051339112552	42.459116263292636	24.872530661851837	9.89530173574298
7	90.8738494675341	2.9591367983292085	4.793181759411511	1.3738319747251728
8	90.98664005731557	2.577565396485923	4.800330994367315	1.6354635518311929
9	85.68498797559525	5.387556984726497	6.262919960511885	2.664535079166369
10	40.30533317716565	35.127461732579064	12.839721757859976	11.727483332395314
11	32.922150915786574	25.346775923202003	24.528873021981614	17.202200139029802
12	31.04954571935754	22.82769735310984	27.587985026155554	18.534771901377063
13	25.595934366980877	26.33880832901083	28.7722862188609	19.29297108514739
14	21.472384939147844	28.791452252997736	29.37076845149092	20.365394356363502
15	20.883067417285634	26.035764428144866	33.953998475843946	19.12716967872555
16	24.867054652098453	24.700987050758048	31.406170854546534	19.025787442596968
17	24.340749270245645	24.790504599087637	27.611638346062524	23.257107784604198
18	24.94543004169373	25.39111639106619	29.48557451708439	20.177879050155685
19	26.920862532380713	26.747645696084497	26.119349632574956	20.212142138959834
20	28.85050949707414	26.63774522256177	25.81486067357962	18.696884606784472
21	27.045631893497696	26.199550358754692	27.660656238924393	19.09416150882322
22	26.395241651747074	23.617269509425583	27.755649797007358	22.23183904181998
23	23.769114696545397	26.744565440598222	27.63894233913841	21.847377523717967
24	23.501246552775804	25.973626928582185	30.182168591129166	20.342957927512842
25	23.548286997671177	27.01798564981222	28.008534969646178	21.425192382870435
26	22.633489146092334	28.92371309968011	26.555110714269414	21.88768703995814
27	21.732001040441855	28.392388042220034	28.0399459700371	21.835664947301012
28	20.763926177304068	27.879278322759056	29.857676985395788	21.499118514541088
29	21.890767295444416	26.24571616320334	29.871975455307393	21.991541086044847
30	22.88443489861016	26.57260352320383	29.357344622026034	21.18561695615998
31	25.246040160447087	26.90017538442349	26.386457219434355	21.467327235695066
32	25.59833012124798	26.394405039145862	26.811418393004093	21.195846446602058
33	25.227748766756967	26.782060896270686	26.19985458151877	21.790335755453576
34	22.46677507137827	27.40735275998497	28.125052288287577	22.000819880349187
35	22.255036027580836	27.645483128566063	28.177720854318366	21.921759989534735
36	24.541194043926726	28.216775451656723	25.86022789327257	21.381802611143986
37	22.82803960371943	29.444618527470556	26.23944156869426	21.487900300115754
38	22.964559569098878	29.052589468112128	25.778848303882036	22.20400265890696
39	22.53975050691118	28.008078635500063	26.136576246590803	23.31559461099796
40	24.20103496584339	26.60660041708941	26.892303620403002	22.300060996664197
41	21.120056950501436	26.68596453066794	27.516492676597508	24.677485842233114
42	22.526212593909765	26.98801970755066	26.352118074939195	24.133649623600384
43	22.302228583858245	26.535146095376877	27.566423237751614	23.596202083013264
44	22.43646687850712	26.799781872278157	27.528775670697108	23.234975578517613
45	21.903240428771564	27.96655222820358	26.288231294483072	23.841976048541785
46	22.24720229140586	28.04610648100965	27.03928124329759	22.667409984286895
47	21.975683474467345	27.501167454857146	28.065728849292604	22.457420221382907
48	22.140306017678384	26.722623373739186	28.73539920871659	22.401671399865837
49	22.68942810683695	26.152928220159932	28.218296565477104	22.939347107526014
50	21.553232138701244	27.819498549617972	27.961190301986726	22.66607900969406
51	21.447552756030074	27.749451258189296	27.43587364411717	23.36712234166346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	936.0
1	1764.5
2	2593.0
3	9346.5
4	16100.0
5	12538.5
6	8977.0
7	8264.0
8	7551.0
9	8040.0
10	8529.0
11	8847.0
12	9165.0
13	9149.0
14	9133.0
15	9117.5
16	9102.0
17	9087.5
18	9073.0
19	9323.5
20	9574.0
21	10556.0
22	11538.0
23	12773.0
24	14008.0
25	16460.0
26	22106.5
27	25301.0
28	31493.5
29	37686.0
30	44666.0
31	51646.0
32	59215.5
33	66785.0
34	75324.5
35	83864.0
36	89194.0
37	94524.0
38	103019.0
39	111514.0
40	123991.0
41	136468.0
42	148782.5
43	161097.0
44	166003.0
45	170909.0
46	177278.5
47	183648.0
48	187225.5
49	190803.0
50	185879.0
51	180955.0
52	173548.0
53	166141.0
54	157876.0
55	149611.0
56	144224.5
57	138838.0
58	131511.5
59	124185.0
60	117653.0
61	111121.0
62	102157.5
63	93194.0
64	82196.5
65	71199.0
66	60935.5
67	50672.0
68	41970.0
69	33268.0
70	28586.5
71	23905.0
72	19376.0
73	14847.0
74	12508.0
75	8138.0
76	6107.0
77	4535.5
78	2964.0
79	2285.0
80	1606.0
81	1230.5
82	855.0
83	568.0
84	281.0
85	214.5
86	148.0
87	113.0
88	78.0
89	54.5
90	31.0
91	29.0
92	27.0
93	19.0
94	11.0
95	6.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2629652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.561012145287137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9897903555687	15.829880145639585
2	6.45832729535116	2.6557949191590926
3	2.195350740819406	1.354158997354588
4	1.2564366173047248	1.0333443419274375
5	0.8610951574485843	0.885249399527414
6	0.6837875523371196	0.8435618501039809
7	0.5624224188566191	0.8094781929424903
8	0.4988611752486102	0.8205672546479112
9	0.42276657538236234	0.7823257821952383
>10	7.774512114558238	41.782558038069325
>50	2.193593720589685	29.55246260292685
>100	0.10058908480503631	2.904995519445156
>500	0.0018978397921378127	0.2791208396851246
>1k	3.7956795842756255E-4	0.14110176936969251
>5k	1.8978397921378128E-4	0.325400347006047
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8339	0.3171142037045206	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02448993250817979	0.0
2	0.0	0.0	0.0	0.08263450829235199	0.0
3	0.0	0.0	0.0	0.1332115428201146	0.0
4	0.0	0.0	0.0	0.22166431147543478	0.0
5	0.0	0.0	0.0	0.42313583698527407	0.0
6	0.0	0.0	0.0	0.7408584862179483	0.0
7	0.0	0.0	0.0	0.9046824446732876	0.0
8	0.0	0.0	0.0	1.2563639599460308	0.0
9	0.0	0.0	0.0	1.4366920033525348	0.0
10	0.0	0.0	0.0	1.7107967137857025	0.0
11	0.0	0.0	0.0	1.9905295453542902	0.0
12	0.0	0.0	0.0	2.196944690780377	0.0
13	0.0	0.0	0.0	2.3073395262947343	0.0
14	0.0	0.0	0.0	2.3742685343916228	0.0
15	0.0	0.0	0.0	2.4434792132190877	0.0
16	0.0	0.0	0.0	2.561897924135969	0.0
17	0.0	0.0	0.0	2.6958319960207664	0.0
18	0.0	0.0	0.0	2.850339132326255	0.0
19	0.0	0.0	0.0	2.9490594192691657	0.0
20	0.0	0.0	0.0	3.052076852754661	0.0
21	0.0	0.0	0.0	3.184794033583151	0.0
22	0.0	0.0	0.0	3.331163210949586	0.0
23	0.0	0.0	0.0	3.487837934449121	0.0
24	0.0	0.0	0.0	3.6209353937327067	0.0
25	0.0	0.0	0.0	3.7401146615597805	0.0
26	0.0	0.0	0.0	3.853779891787963	0.0
27	0.0	0.0	0.0	3.98486187525954	0.0
28	0.0	0.0	0.0	4.109935459140601	0.0
29	0.0	1.1408353652878784E-4	0.0	4.24645542452005	0.0
30	0.0	1.1408353652878784E-4	0.0	4.413511749843706	0.0
31	0.0	1.1408353652878784E-4	0.0	4.557028838796921	0.0
32	3.8027845509595945E-5	1.1408353652878784E-4	0.0	4.70301773770826	0.0
33	3.8027845509595945E-5	1.1408353652878784E-4	0.0	4.845736241905773	0.0
34	3.8027845509595945E-5	1.1408353652878784E-4	0.0	4.998608180854349	0.0
35	3.8027845509595945E-5	1.1408353652878784E-4	0.0	5.16414339235762	0.0
36	3.8027845509595945E-5	1.1408353652878784E-4	0.0	5.316977303460686	0.0
37	3.8027845509595945E-5	1.1408353652878784E-4	0.0	5.483767433865774	0.0
38	3.8027845509595945E-5	1.1408353652878784E-4	0.0	5.669571487025659	0.0
39	3.8027845509595945E-5	1.1408353652878784E-4	0.0	5.932990372870631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGAT	4650	0.0	39.09677	7
CGTAAGG	455	0.0	39.065933	2
ACGGGAT	1305	0.0	38.965515	5
ATAACGG	210	0.0	38.57143	2
TACGGGA	960	0.0	38.437496	4
CGTTTTT	5810	0.0	38.106712	1
TCGATAG	155	0.0	37.741936	1
CGTTAGG	295	0.0	37.372883	2
AGGGCGA	2645	0.0	37.344044	6
TAGGGAT	4190	0.0	37.321	5
TAAGGGA	3670	0.0	36.968662	4
GGCGATT	1235	0.0	36.80162	8
AGGGATT	4415	0.0	36.693092	6
ACGTAAG	135	0.0	36.666664	1
CGAATAT	345	0.0	36.52174	14
CACGACC	745	0.0	36.24161	27
ATAGGGA	4140	0.0	36.195652	4
TTAGGGA	3535	0.0	36.025463	4
AGGGATC	2530	0.0	36.017784	6
GACCGAT	1450	0.0	36.0	9
>>END_MODULE