FastQCFastQC Report
Thu 26 May 2016
SRR1547976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547976_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2288258
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.232010551257769No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGC302.1664491E-644.99999641
GGCGATA8600.040.029078
TACGGGT1750.039.8571434
CGTAAGG3250.039.4615362
GGGCGAT36600.038.7295077
ATCGTAG356.2510044E-638.5714261
TAAGGGA29800.037.6761744
ACGGGAT8450.037.5443765
CATTACG301.1402756E-437.49999644
TACGATA301.1402756E-437.49999642
CGCGTAA601.5643309E-1037.49999631
TACGGGA8550.037.368424
CTCACGA2500.036.924
AAGGGAT38700.036.5116275
AGGGCGA18750.036.486
TAGGGAC20800.036.4543275
ATAGGGT5500.036.4090924
ATAGGGC9150.036.3934444
AGGGATG40850.036.242356
AGGGACC20450.036.1980446
Produced by FastQC (version 0.11.5)