##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547976_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2288258
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.967656619139973	31.0	31.0	33.0	30.0	33.0
2	31.33366167626203	31.0	31.0	33.0	30.0	34.0
3	31.548511575180772	31.0	31.0	34.0	30.0	34.0
4	35.426538441032434	37.0	35.0	37.0	33.0	37.0
5	35.25817062586474	37.0	35.0	37.0	33.0	37.0
6	35.27810544090745	37.0	35.0	37.0	32.0	37.0
7	35.568760603043884	36.0	35.0	37.0	35.0	37.0
8	35.63879772298404	37.0	35.0	37.0	35.0	37.0
9	37.46783623175359	39.0	37.0	39.0	35.0	39.0
10	36.68416498489244	39.0	37.0	39.0	32.0	39.0
11	36.31781556100754	38.0	35.0	39.0	32.0	39.0
12	35.75790448454676	37.0	35.0	39.0	31.0	39.0
13	35.50695376133286	37.0	35.0	39.0	30.0	39.0
14	36.44494545632529	38.0	35.0	40.0	30.0	41.0
15	36.6762629039208	38.0	35.0	40.0	31.0	41.0
16	36.81310936091997	38.0	35.0	40.0	31.0	41.0
17	36.76319671995029	38.0	35.0	40.0	31.0	41.0
18	36.6810844756142	38.0	35.0	40.0	31.0	41.0
19	36.6184647884985	38.0	35.0	40.0	31.0	41.0
20	36.51095549540305	38.0	35.0	40.0	30.0	41.0
21	36.414030236101	38.0	35.0	40.0	30.0	41.0
22	36.29330914608405	38.0	35.0	40.0	30.0	41.0
23	36.19811664593765	38.0	34.0	40.0	30.0	41.0
24	36.09465147723727	38.0	34.0	40.0	30.0	41.0
25	35.91357180877331	38.0	34.0	40.0	30.0	41.0
26	35.66982132259562	38.0	34.0	40.0	29.0	41.0
27	35.77220182339579	38.0	34.0	40.0	29.0	41.0
28	35.80324115549907	38.0	34.0	40.0	29.0	41.0
29	35.7924351187672	38.0	34.0	40.0	29.0	41.0
30	35.73168453906858	38.0	34.0	40.0	29.0	41.0
31	35.61951886544262	38.0	34.0	40.0	29.0	41.0
32	35.51391451488425	38.0	34.0	40.0	29.0	41.0
33	35.4139965860493	38.0	34.0	40.0	28.0	41.0
34	35.28007680952061	38.0	34.0	40.0	27.0	41.0
35	35.12494264195733	38.0	34.0	40.0	26.0	41.0
36	35.048188185073535	37.0	34.0	40.0	26.0	41.0
37	35.01627307759877	37.0	33.0	40.0	26.0	41.0
38	34.91503711557001	37.0	33.0	40.0	26.0	41.0
39	34.85329713694872	37.0	33.0	40.0	25.0	41.0
40	34.64695982708243	37.0	33.0	40.0	24.0	41.0
41	34.56708159656822	37.0	33.0	40.0	24.0	41.0
42	34.520006922296346	37.0	33.0	40.0	24.0	41.0
43	34.47889748446198	37.0	33.0	40.0	24.0	41.0
44	34.32064959458243	36.0	33.0	40.0	24.0	41.0
45	34.1889459143156	36.0	33.0	40.0	23.0	41.0
46	34.12941897286058	36.0	33.0	40.0	23.0	41.0
47	34.05815209648562	36.0	33.0	40.0	23.0	41.0
48	33.95284141910571	36.0	33.0	40.0	23.0	41.0
49	33.80809462918954	36.0	33.0	40.0	23.0	41.0
50	33.67764648916337	35.0	32.0	39.0	23.0	41.0
51	33.33988300270337	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	9.0
14	35.0
15	97.0
16	270.0
17	646.0
18	1339.0
19	2510.0
20	4164.0
21	6660.0
22	9775.0
23	14194.0
24	20042.0
25	26940.0
26	35041.0
27	41924.0
28	48949.0
29	58980.0
30	71055.0
31	87646.0
32	108628.0
33	138466.0
34	207253.0
35	233180.0
36	202737.0
37	251172.0
38	333072.0
39	383424.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.29663875314759	24.700448987832665	29.850742355101566	12.152169903918177
2	30.03114159329936	26.235503164415903	30.125929855811712	13.607425386473029
3	29.663132391539765	26.25980112382432	29.870757580657425	14.206308903978485
4	26.528695627853153	28.95141194742901	29.312166722458745	15.207725702259097
5	23.8185991264971	33.590748945267535	28.060909215656626	14.529742712578738
6	22.68808849351778	41.95912349044557	25.047481533987863	10.30530648204879
7	91.32077763958435	3.1476345761710434	4.245325483402658	1.2862623008419505
8	91.62384661170199	2.6691920229274846	4.127375496993783	1.5795858683767303
9	86.31836969432642	5.469662948845803	5.746817011018862	2.4651503458089077
10	43.358834537014616	33.86807781290396	11.699336351058315	11.0737512990231
11	36.74729860007045	23.97893943777319	22.995309095390468	16.278452866765896
12	34.365661564386535	21.55753415917261	26.223310483345845	17.85349379309501
13	26.621429926170915	27.18535235100238	27.38873850763332	18.804479215193393
14	20.689974644467537	30.6434851314843	28.973743345374515	19.692796878673647
15	19.77263927406787	25.63176879530193	36.30355493130582	18.292036999324377
16	23.998080636012197	23.77751984260516	33.77682936102485	18.44757016035779
17	24.362200416211806	23.43699879996049	27.044633952989567	25.156166830838135
18	25.138861089964504	25.06614201720261	29.673489615244435	20.121507277588453
19	28.252889315802676	25.46513548734452	25.712791127573905	20.5691840692789
20	29.104454130609398	26.346111321363235	25.86238090285274	18.687053645174625
21	27.0377728385523	25.316987857138486	28.514747899930864	19.130491404378354
22	26.33855098507249	23.393166330020478	27.84297050420014	22.425312180706896
23	24.056640466241134	26.211685920031748	27.5339581463279	22.197715467399217
24	23.749725773929338	24.969693102788234	31.176641794762656	20.103939328519775
25	23.823493679471458	25.66257825822088	28.878692874667106	21.635235187640557
26	22.037637364318186	29.589888902387756	26.080101107480015	22.292372625814046
27	21.588168816628194	29.152700438499508	28.477295829403854	20.781834915468448
28	20.338266052167196	27.68800546092268	30.963248025353785	21.010480461556348
29	21.589261350774258	25.956120332584874	30.800810048517256	21.653808268123612
30	23.227319646648237	26.503436238396194	29.124207147970203	21.145036966985366
31	26.092993010403546	26.244680451242825	26.527471989609563	21.134854548744066
32	27.545713813739535	25.977796210042747	26.622828369877872	19.853661606339845
33	26.612645951636573	26.005939889645312	26.293888189181462	21.087525969536653
34	22.80389711300037	26.448896933824766	28.58445157844963	22.16275437472523
35	22.8107145260718	26.551420338091248	28.976015816398327	21.66184931943863
36	26.755112404283082	26.364859207309664	26.512613525223117	20.367414863184134
37	23.693263609260846	28.94520635347937	26.96667071632657	20.394859320933218
38	23.751211620367982	28.102338110475305	26.06974388377534	22.07670638538137
39	23.09162690570731	27.794986404505085	27.18994099441584	21.92344569537176
40	24.764558891523595	25.734292199568404	27.486848073949705	22.014300834958295
41	21.17269993156366	25.112989881385754	28.664556182038915	25.04975400501167
42	22.806956208609343	26.01323801774101	27.260999415275727	23.918806358373924
43	22.79952697641612	25.621236766133887	27.794418286749135	23.784817970700857
44	22.488504355715133	26.64419833777485	28.034863201614503	22.832434104895512
45	21.90806281459521	28.630818727608514	25.824404415935614	23.636714041860664
46	22.63105821109333	28.445131624143784	27.080469072980407	21.843341091782484
47	22.80446523075632	26.69196393064069	27.990637419381905	22.512933419221085
48	22.913063124874906	25.765582377511624	29.407872713653795	21.913481783959675
49	23.338714428180737	24.832383411311138	29.29822598675499	22.530676173753132
50	21.942674296342457	27.81543864371937	27.857916371318268	22.383970688619904
51	21.478696895192762	28.575449097086082	26.774952824375575	23.170901183345585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1031.0
1	1750.5
2	2470.0
3	6632.0
4	10794.0
5	8558.0
6	6322.0
7	6106.5
8	5891.0
9	6277.0
10	6663.0
11	7009.0
12	7355.0
13	7568.5
14	7782.0
15	7832.0
16	7882.0
17	7530.5
18	7179.0
19	7339.0
20	7499.0
21	7729.5
22	7960.0
23	9706.5
24	11453.0
25	14185.5
26	19631.0
27	22344.0
28	26969.0
29	31594.0
30	36683.5
31	41773.0
32	48333.5
33	54894.0
34	61720.5
35	68547.0
36	71968.5
37	75390.0
38	84929.5
39	94469.0
40	110134.0
41	125799.0
42	138574.5
43	151350.0
44	155840.5
45	160331.0
46	163106.5
47	165882.0
48	166021.0
49	166160.0
50	163583.0
51	161006.0
52	152150.0
53	143294.0
54	135954.5
55	128615.0
56	124114.0
57	119613.0
58	115418.5
59	111224.0
60	103265.5
61	95307.0
62	87979.0
63	80651.0
64	70498.0
65	60345.0
66	51403.0
67	42461.0
68	35479.0
69	28497.0
70	23967.0
71	19437.0
72	16751.5
73	14066.0
74	10885.0
75	6379.5
76	5055.0
77	3681.5
78	2308.0
79	1894.0
80	1480.0
81	1091.0
82	702.0
83	575.0
84	448.0
85	312.0
86	176.0
87	126.5
88	77.0
89	59.0
90	41.0
91	25.5
92	10.0
93	6.5
94	3.0
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2288258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.28053230916766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77307088789442	15.18786738346403
2	6.758464466491869	2.6061358501311616
3	2.200775534664511	1.2729637140397452
4	1.1627321735457836	0.896723809558329
5	0.7231047194733389	0.6970921953358664
6	0.5614284946860164	0.6494784138648663
7	0.41801750562973605	0.564172001616426
8	0.34893943151721957	0.5382190386648279
9	0.29207865952285167	0.506828882857385
>10	5.580171305354148	28.92527972329454
>50	2.7622281792389822	37.532712653464586
>100	0.4159743357515444	9.945011232805752
>500	0.0020868273896984713	0.24784848033014237
>1k	6.956091298994905E-4	0.19232398311332474
>5k	2.3186970996649683E-4	0.23734263745900536
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5309	0.232010551257769	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02359873755494354	0.0
2	0.0	0.0	0.0	0.09155436144001244	0.0
3	0.0	0.0	0.0	0.1377030037696798	0.0
4	0.0	0.0	0.0	0.23410821681820843	0.0
5	0.0	0.0	0.0	0.40873887472479065	0.0
6	0.0	0.0	0.0	0.6399628013973949	0.0
7	0.0	0.0	0.0	0.7470311477114906	0.0
8	0.0	0.0	0.0	1.008277912717884	0.0
9	0.0	0.0	0.0	1.0988271427435192	0.0
10	0.0	0.0	0.0	1.2755117648446985	0.0
11	0.0	0.0	0.0	1.5187972684898294	0.0
12	0.0	0.0	0.0	1.7089856126363374	0.0
13	0.0	0.0	0.0	1.7955580183703062	0.0
14	4.370136584248804E-5	0.0	0.0	1.838166850066732	0.0
15	4.370136584248804E-5	0.0	0.0	1.8987806444902628	0.0
16	4.370136584248804E-5	0.0	0.0	2.021362975678442	0.0
17	4.370136584248804E-5	0.0	0.0	2.1622561791546233	0.0
18	4.370136584248804E-5	0.0	0.0	2.3200181098460053	0.0
19	4.370136584248804E-5	0.0	0.0	2.4226289168441673	0.0
20	4.370136584248804E-5	0.0	0.0	2.536165065302951	0.0
21	4.370136584248804E-5	0.0	0.0	2.6723385212681436	0.0
22	4.370136584248804E-5	0.0	0.0	2.8302752574228953	0.0
23	4.370136584248804E-5	0.0	0.0	2.9894793331870795	0.0
24	4.370136584248804E-5	0.0	0.0	3.114334135399068	0.0
25	4.370136584248804E-5	0.0	0.0	3.233158149124793	0.0
26	4.370136584248804E-5	0.0	0.0	3.344684034754822	0.0
27	4.370136584248804E-5	0.0	0.0	3.475220014526334	0.0
28	4.370136584248804E-5	0.0	0.0	3.5985015675679928	0.0
29	4.370136584248804E-5	0.0	0.0	3.7367289877277825	0.0
30	4.370136584248804E-5	0.0	0.0	3.907645029537753	0.0
31	4.370136584248804E-5	0.0	0.0	4.059113963547817	0.0
32	4.370136584248804E-5	0.0	0.0	4.201667818926013	0.0
33	4.370136584248804E-5	0.0	0.0	4.345183104352744	0.0
34	4.370136584248804E-5	0.0	0.0	4.4950350878266345	0.0
35	4.370136584248804E-5	0.0	0.0	4.6751284164635285	0.0
36	4.370136584248804E-5	0.0	0.0	4.836823470080734	0.0
37	4.370136584248804E-5	0.0	0.0	5.003893791696566	0.0
38	4.370136584248804E-5	0.0	0.0	5.187745437795913	0.0
39	4.370136584248804E-5	0.0	0.0	5.488192327963018	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCGC	30	2.1664491E-6	44.999996	41
GGCGATA	860	0.0	40.02907	8
TACGGGT	175	0.0	39.857143	4
CGTAAGG	325	0.0	39.461536	2
GGGCGAT	3660	0.0	38.729507	7
ATCGTAG	35	6.2510044E-6	38.571426	1
TAAGGGA	2980	0.0	37.676174	4
ACGGGAT	845	0.0	37.544376	5
CATTACG	30	1.1402756E-4	37.499996	44
TACGATA	30	1.1402756E-4	37.499996	42
CGCGTAA	60	1.5643309E-10	37.499996	31
TACGGGA	855	0.0	37.36842	4
CTCACGA	250	0.0	36.9	24
AAGGGAT	3870	0.0	36.511627	5
AGGGCGA	1875	0.0	36.48	6
TAGGGAC	2080	0.0	36.454327	5
ATAGGGT	550	0.0	36.409092	4
ATAGGGC	915	0.0	36.393444	4
AGGGATG	4085	0.0	36.24235	6
AGGGACC	2045	0.0	36.198044	6
>>END_MODULE