FastQCFastQC Report
Thu 26 May 2016
SRR1547975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547975_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2074194
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.21902483567110886No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23210.11189888698935586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG453.8562575E-1045.0000049
TCGTTAA302.166351E-644.99999612
TATAGCG1600.043.5937541
TACGGGA8400.042.053574
TAACGCG650.041.538461
ACGGGAC6900.039.1304325
AGGGCGA19900.039.1206056
GGGCGAT39200.038.6862267
TATCACG356.2507224E-638.5714261
CGAACGC356.2507224E-638.57142614
TATCGTG356.2507224E-638.5714261
TAAGGGA26550.037.9661034
CGTAAGG3500.037.928572
GTTACGG1550.037.7419362
TAGGGCG8650.037.7167635
CGGTCTA2450.037.6530631
AAGGGAT28250.037.5929225
AACACGT9050.037.5414441
TCGTTAG601.5643309E-1037.4999961
CGGATTA301.1402389E-437.49999617
Produced by FastQC (version 0.11.5)