##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547975_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2074194
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.921901230068162	31.0	31.0	31.0	30.0	33.0
2	31.274054403782866	31.0	31.0	33.0	30.0	34.0
3	31.48104613165403	31.0	31.0	34.0	30.0	34.0
4	35.344902164406996	37.0	35.0	37.0	33.0	37.0
5	35.1750757161577	37.0	35.0	37.0	32.0	37.0
6	35.203814590149236	37.0	35.0	37.0	32.0	37.0
7	35.54543258730861	35.0	35.0	37.0	35.0	37.0
8	35.618194826520565	37.0	35.0	37.0	35.0	37.0
9	37.43533343554171	39.0	37.0	39.0	35.0	39.0
10	36.60459050599896	39.0	35.0	39.0	32.0	39.0
11	36.23835475370192	38.0	35.0	39.0	31.0	39.0
12	35.6122947033884	37.0	35.0	39.0	30.0	39.0
13	35.33101194970191	37.0	35.0	39.0	30.0	39.0
14	36.24476013333372	38.0	35.0	40.0	30.0	41.0
15	36.48210726672625	38.0	35.0	40.0	30.0	41.0
16	36.609660427134585	38.0	35.0	40.0	31.0	41.0
17	36.53088524988502	38.0	35.0	40.0	31.0	41.0
18	36.45809408377423	38.0	35.0	40.0	30.0	41.0
19	36.4233017740867	38.0	35.0	40.0	30.0	41.0
20	36.31505828288	38.0	34.0	40.0	30.0	41.0
21	36.18074876313402	38.0	34.0	40.0	30.0	41.0
22	36.06755346896192	38.0	34.0	40.0	30.0	41.0
23	35.985736628299954	38.0	34.0	40.0	30.0	41.0
24	35.89301193620269	38.0	34.0	40.0	30.0	41.0
25	35.732795967975996	37.0	34.0	40.0	29.0	41.0
26	35.46094193696443	37.0	34.0	40.0	29.0	41.0
27	35.580002159875114	37.0	34.0	40.0	29.0	41.0
28	35.609868700806196	37.0	34.0	40.0	29.0	41.0
29	35.62581465378841	37.0	34.0	40.0	29.0	41.0
30	35.52500151866219	37.0	34.0	40.0	29.0	41.0
31	35.43645290652658	37.0	34.0	40.0	29.0	41.0
32	35.33497975599197	37.0	34.0	40.0	28.0	41.0
33	35.21625267453285	37.0	34.0	40.0	27.0	41.0
34	35.077047277159224	37.0	34.0	40.0	26.0	41.0
35	34.95687818979324	37.0	33.0	40.0	26.0	41.0
36	34.88964291671849	37.0	33.0	40.0	25.0	41.0
37	34.83590011348986	37.0	33.0	40.0	25.0	41.0
38	34.759724500215505	37.0	33.0	40.0	25.0	41.0
39	34.716203980919815	37.0	33.0	40.0	25.0	41.0
40	34.496469471997315	37.0	33.0	40.0	24.0	41.0
41	34.43825119540409	37.0	33.0	40.0	24.0	41.0
42	34.403247719355086	36.0	33.0	40.0	24.0	41.0
43	34.366130169116296	36.0	33.0	40.0	24.0	41.0
44	34.22332819398764	36.0	33.0	40.0	24.0	41.0
45	34.069714790419795	36.0	33.0	40.0	23.0	41.0
46	34.01103802248006	36.0	33.0	40.0	23.0	41.0
47	33.94582811443867	35.0	33.0	40.0	23.0	41.0
48	33.8651625643503	35.0	33.0	40.0	23.0	41.0
49	33.725808675562654	35.0	32.0	39.0	23.0	41.0
50	33.600012824258485	35.0	32.0	39.0	23.0	41.0
51	33.246127411418605	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	14.0
14	40.0
15	105.0
16	277.0
17	654.0
18	1307.0
19	2459.0
20	4138.0
21	6519.0
22	9340.0
23	13680.0
24	19061.0
25	25457.0
26	32298.0
27	39769.0
28	47026.0
29	55915.0
30	68587.0
31	83462.0
32	101911.0
33	130041.0
34	195428.0
35	218440.0
36	178558.0
37	218967.0
38	289002.0
39	331691.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.383816557178356	23.934791056188573	29.03161420773563	11.649778178897442
2	30.352271773999924	26.566271043113616	29.359307760026304	13.722149422860157
3	30.210963873196047	26.611975543271267	29.782411866970975	13.394648716561711
4	26.485516783868817	28.955873944288722	28.949365392051078	15.609243879791379
5	24.040374236932514	33.41138774868696	27.79166268921808	14.75657532516245
6	22.3134383765453	43.00836855183267	24.409288620061574	10.268904451560463
7	91.2934855659596	3.3275575958661534	4.220964866352906	1.1579919718213436
8	91.66138750762947	2.7464644097900193	3.9292370916124533	1.662910990968058
9	86.63707444915953	5.462989479286894	5.579082766607173	2.3208533049464033
10	43.47592365998552	34.578877385625454	11.053835851419876	10.891363102969153
11	37.47127800003278	24.541484547732757	21.644021726029482	16.343215726204974
12	34.73093645049595	22.30789405426879	25.39882961767318	17.56233987756208
13	25.651023964007223	30.14920494418555	26.143745474145618	18.056025617661607
14	20.480822912418027	32.640775163750355	28.187238030772434	18.691163893059183
15	20.014039188234083	26.200152926871834	36.37653951366169	17.409268371232393
16	24.106857892752558	23.786878180150943	34.34616048450627	17.760103442590232
17	23.61755939897618	24.096878112654842	26.396373723962174	25.8891887644068
18	25.098713042270877	25.20371768503814	29.50249590925439	20.19507336343659
19	27.931379610586088	26.72884021456045	25.18202251091267	20.15775766394079
20	30.391082029935486	26.57142967340567	24.56877225563279	18.468716041026056
21	27.10199720951849	26.593414116519476	27.577121522866232	18.7274671510958
22	26.426120218263094	23.691708682987223	26.934606888265993	22.947564210483687
23	23.70718457386339	27.473032898562046	26.720162144910265	22.099620382664302
24	23.274872070789907	25.586468768109444	31.39175988359816	19.74689927750249
25	23.19141796765394	26.297347306953927	29.09727826808871	21.413956457303414
26	21.723763543815092	30.042030784005743	26.252703459753523	21.981502212425646
27	21.35740437008303	30.175480210626393	27.110289587184226	21.356825832106352
28	19.114846537980537	29.186855231477864	31.164587304755486	20.533710925786114
29	21.397419913470003	27.115978543954906	29.47135128150983	22.015250261065262
30	23.05001364385395	27.421591230135654	29.408965603024594	20.119429522985797
31	27.012709515117677	26.593221270527252	25.81291817448127	20.581151039873802
32	27.528138640840734	25.82217478210814	26.680098390025236	19.9695881870259
33	26.59813884332902	26.499980233285797	25.92071908413581	20.981161839249367
34	21.907690408900997	27.47592558844544	29.27561259940006	21.340771403253505
35	21.86434827214812	27.98176062605523	28.817121252881844	21.33676984891481
36	26.94492414885011	27.85771244155561	25.861804633510655	19.335558776083626
37	23.358856500404496	29.388572139346657	26.78886352964091	20.463707830607937
38	22.968295154648022	30.08662641970809	25.674069060078274	21.271009365565615
39	23.268893845030888	28.53518041224688	25.916476472306833	22.279449270415398
40	24.785675785389408	26.08348110157488	26.474090658829407	22.65675245420631
41	20.65250405699756	26.09071282628337	27.5345025585842	25.72228055813487
42	22.795505145613188	26.163030073368258	26.062943003402765	24.978521777615786
43	22.494568974743924	26.327527704737356	26.709555615337816	24.468347705180904
44	22.08149285939502	27.46421019441769	27.136950545609523	23.317346400577765
45	21.394961127069116	29.129965663771085	25.87568954495095	23.599383664208844
46	22.94452688610612	28.354290871538534	26.54298488955228	22.158197352803064
47	22.202600142513187	26.98619319118655	28.592648517930336	22.218558148369922
48	23.356735194490007	25.773818649557374	28.91050692461747	21.958939231335158
49	23.567130172008984	25.04953731425315	28.833657796715258	22.54967471702261
50	21.68924411120657	28.266353099083307	27.88475909196536	22.159643697744762
51	20.917811930803	28.449942483682815	26.77695528962093	23.855290295893248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1222.0
1	1733.0
2	2244.0
3	5793.0
4	9342.0
5	7310.5
6	5279.0
7	5308.0
8	5337.0
9	5631.0
10	5925.0
11	6350.0
12	6775.0
13	7109.0
14	7443.0
15	7363.5
16	7284.0
17	7397.5
18	7511.0
19	7710.5
20	7910.0
21	8315.5
22	8721.0
23	9695.0
24	10669.0
25	12018.0
26	16907.0
27	20447.0
28	23237.0
29	26027.0
30	32609.0
31	39191.0
32	45079.5
33	50968.0
34	57272.0
35	63576.0
36	64733.5
37	65891.0
38	75546.5
39	85202.0
40	97699.5
41	110197.0
42	124767.0
43	139337.0
44	144287.0
45	149237.0
46	151575.0
47	153913.0
48	153908.0
49	153903.0
50	151540.5
51	149178.0
52	141656.0
53	134134.0
54	125697.5
55	117261.0
56	113385.0
57	109509.0
58	104441.5
59	99374.0
60	93395.5
61	87417.0
62	77930.0
63	68443.0
64	59833.0
65	51223.0
66	43210.5
67	35198.0
68	28981.5
69	22765.0
70	19223.0
71	15681.0
72	13288.5
73	10896.0
74	9551.5
75	6083.0
76	3959.0
77	3143.5
78	2328.0
79	1608.0
80	888.0
81	636.0
82	384.0
83	301.0
84	218.0
85	163.5
86	109.0
87	79.0
88	49.0
89	35.5
90	22.0
91	20.0
92	18.0
93	11.5
94	5.0
95	4.0
96	3.0
97	5.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2074194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.949811107250635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96650966693059	15.75366951654146
2	6.888805795996676	2.7486074876933406
3	2.266456227349403	1.356461208554175
4	1.17456384057109	0.9372930701120037
5	0.7680373869807158	0.7661100396785818
6	0.5090459524566281	0.609322235785213
7	0.40910354671737736	0.5713076936222574
8	0.3389398401429918	0.540942863005953
9	0.2907735009483024	0.5220788777021336
>10	5.374978159864514	28.92887429097798
>50	2.634291094993766	36.8524298426341
>100	0.3745420368829721	9.638787419529853
>500	0.0034588313943986035	0.43580344108675106
>1k	4.94118770628372E-4	0.33831201307616593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4543	0.21902483567110886	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2321	0.11189888698935586	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031578531227069405	0.0
2	0.0	0.0	0.0	0.13316015763231404	0.0
3	0.0	0.0	0.0	0.1933763187050006	0.0
4	0.0	0.0	0.0	0.328513147757635	0.0
5	0.0	0.0	0.0	0.5579034555109117	0.0
6	0.0	0.0	0.0	0.8567665319637411	0.0
7	0.0	0.0	0.0	0.9899749010941118	0.0
8	0.0	0.0	0.0	1.333433613249291	0.0
9	0.0	0.0	0.0	1.4558426068149846	0.0
10	0.0	0.0	0.0	1.702251573382239	0.0
11	0.0	0.0	0.0	2.036164408922213	0.0
12	0.0	0.0	0.0	2.2853696423767498	0.0
13	0.0	0.0	0.0	2.403777081603746	0.0
14	0.0	0.0	0.0	2.4610041297969234	0.0
15	0.0	0.0	0.0	2.5370818737302296	0.0
16	0.0	0.0	0.0	2.7002295831537455	0.0
17	0.0	0.0	0.0	2.8813601813523713	0.0
18	0.0	0.0	0.0	3.094744271750858	0.0
19	0.0	0.0	0.0	3.2236136060561353	0.0
20	0.0	0.0	0.0	3.35421855429145	0.0
21	0.0	0.0	0.0	3.5153413807965888	0.0
22	0.0	0.0	0.0	3.7060178556104204	0.0
23	0.0	0.0	0.0	3.897610348887327	0.0
24	0.0	0.0	0.0	4.053960237084863	0.0
25	0.0	0.0	0.0	4.182588513899857	0.0
26	0.0	0.0	0.0	4.313434519625455	0.0
27	0.0	0.0	0.0	4.4548388434254464	0.0
28	0.0	0.0	0.0	4.594555764793457	0.0
29	0.0	0.0	0.0	4.745747022698938	0.0
30	0.0	0.0	0.0	4.932711212162411	0.0
31	0.0	0.0	0.0	5.095521441099531	0.0
32	0.0	0.0	0.0	5.255535403149368	0.0
33	0.0	0.0	0.0	5.4148744042264125	0.0
34	0.0	0.0	0.0	5.5828914749536445	0.0
35	0.0	0.0	0.0	5.770337779397684	0.0
36	0.0	0.0	0.0	5.948045361234292	0.0
37	0.0	0.0	0.0	6.133659628752181	0.0
38	0.0	0.0	0.0	6.340583378411084	0.0
39	0.0	0.0	0.0	6.633034325622386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	45	3.8562575E-10	45.000004	9
TCGTTAA	30	2.166351E-6	44.999996	12
TATAGCG	160	0.0	43.593754	1
TACGGGA	840	0.0	42.05357	4
TAACGCG	65	0.0	41.53846	1
ACGGGAC	690	0.0	39.130432	5
AGGGCGA	1990	0.0	39.120605	6
GGGCGAT	3920	0.0	38.686226	7
TATCACG	35	6.2507224E-6	38.571426	1
CGAACGC	35	6.2507224E-6	38.571426	14
TATCGTG	35	6.2507224E-6	38.571426	1
TAAGGGA	2655	0.0	37.966103	4
CGTAAGG	350	0.0	37.92857	2
GTTACGG	155	0.0	37.741936	2
TAGGGCG	865	0.0	37.716763	5
CGGTCTA	245	0.0	37.65306	31
AAGGGAT	2825	0.0	37.592922	5
AACACGT	905	0.0	37.54144	41
TCGTTAG	60	1.5643309E-10	37.499996	1
CGGATTA	30	1.1402389E-4	37.499996	17
>>END_MODULE