FastQCFastQC Report
Thu 26 May 2016
SRR1547974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547974_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4046659
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139180.3439380486470444No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41880.10349278256457982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA3300.042.27272831
GCGTAAG2900.041.120691
CGACGGT3400.041.0294128
ACGGTAC556.184564E-1140.90909220
TACGGGA15750.040.571434
CGTAAGG6800.040.0367622
CGTTTTT89950.039.5719871
ACGGGAC13750.038.9454545
AGGGCGA34650.038.8961076
TAGGGAC51300.038.8596465
CGAATAT2750.038.45454414
TAGGGCG12700.038.2677155
CGTTAGG7300.038.2191772
TAGGGAT70000.038.1535725
ACGGGAT21150.038.0851065
GTAGGGA59200.037.5506744
CGGACCG900.037.56
CGGGTAT2650.037.358496
TAAGGGA63200.037.345734
CGGGATT20800.037.2115366
Produced by FastQC (version 0.11.5)