##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547974_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4046659
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.795884456782744	31.0	31.0	31.0	30.0	33.0
2	31.13820116792643	31.0	31.0	33.0	30.0	34.0
3	31.302029402526873	31.0	31.0	34.0	30.0	34.0
4	35.17960544735793	35.0	35.0	37.0	33.0	37.0
5	34.982821384257974	35.0	35.0	37.0	32.0	37.0
6	35.017429934175325	36.0	35.0	37.0	32.0	37.0
7	35.505413972365844	35.0	35.0	37.0	35.0	37.0
8	35.60551852775339	37.0	35.0	37.0	35.0	37.0
9	37.40739459391068	39.0	37.0	39.0	35.0	39.0
10	36.43383714812639	38.0	35.0	39.0	32.0	39.0
11	35.98277443194497	38.0	35.0	39.0	30.0	39.0
12	35.60673409842539	37.0	35.0	39.0	30.0	39.0
13	35.415827723561584	37.0	35.0	39.0	30.0	39.0
14	36.335155494940395	38.0	35.0	40.0	30.0	41.0
15	36.52854317598789	38.0	35.0	40.0	30.0	41.0
16	36.651774463823116	38.0	35.0	40.0	31.0	41.0
17	36.578419135390455	38.0	35.0	40.0	31.0	41.0
18	36.489531734697685	38.0	35.0	40.0	30.0	41.0
19	36.465207965385765	38.0	35.0	40.0	30.0	41.0
20	36.349323726066366	38.0	34.0	40.0	30.0	41.0
21	36.23902755334709	38.0	34.0	40.0	30.0	41.0
22	36.13335667769387	38.0	34.0	40.0	30.0	41.0
23	36.049513932357534	38.0	34.0	40.0	30.0	41.0
24	35.95427734335905	38.0	34.0	40.0	29.0	41.0
25	35.77795831079416	38.0	34.0	40.0	29.0	41.0
26	35.55140178601656	38.0	34.0	40.0	28.0	41.0
27	35.632297161683255	38.0	34.0	40.0	29.0	41.0
28	35.669473261769774	38.0	34.0	40.0	29.0	41.0
29	35.65903180870936	38.0	34.0	40.0	29.0	41.0
30	35.57253897597994	38.0	34.0	40.0	29.0	41.0
31	35.47616836506362	38.0	34.0	40.0	27.0	41.0
32	35.36987302364741	38.0	34.0	40.0	27.0	41.0
33	35.23949658224229	38.0	34.0	40.0	27.0	41.0
34	35.10711725401127	38.0	34.0	40.0	26.0	41.0
35	34.98269535436517	38.0	33.0	40.0	25.0	41.0
36	34.92289021634885	38.0	33.0	40.0	25.0	41.0
37	34.87501887359424	38.0	33.0	40.0	25.0	41.0
38	34.769536548545354	37.0	33.0	40.0	24.0	41.0
39	34.726864062427794	37.0	33.0	40.0	24.0	41.0
40	34.554119830704785	37.0	33.0	40.0	24.0	41.0
41	34.49553915958819	37.0	33.0	40.0	24.0	41.0
42	34.47771630868823	37.0	33.0	40.0	24.0	41.0
43	34.44084095052239	37.0	33.0	40.0	24.0	41.0
44	34.29195837850434	37.0	33.0	40.0	23.0	41.0
45	34.16457502349469	37.0	33.0	40.0	23.0	41.0
46	34.1088248355001	37.0	33.0	40.0	23.0	41.0
47	34.056939069983414	37.0	33.0	40.0	23.0	41.0
48	33.96792712210245	36.0	33.0	40.0	23.0	41.0
49	33.81091735182035	36.0	32.0	40.0	23.0	41.0
50	33.68756151679694	36.0	32.0	40.0	23.0	41.0
51	33.356067560918774	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	7.0
13	17.0
14	49.0
15	152.0
16	480.0
17	1174.0
18	2464.0
19	4792.0
20	8145.0
21	12606.0
22	19379.0
23	27651.0
24	39062.0
25	53826.0
26	69403.0
27	82601.0
28	95781.0
29	113412.0
30	135784.0
31	163787.0
32	197321.0
33	244495.0
34	340404.0
35	383042.0
36	350947.0
37	427197.0
38	576400.0
39	696212.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.38310912780148	24.771966207184743	31.3168962346469	11.528028430366879
2	29.792033378646433	27.36096616987001	29.714141962542435	13.132858488941125
3	28.757748058336517	26.167438373235797	31.07316430665396	14.001649261773725
4	25.74471928571199	28.657887902094046	30.563904692735417	15.033488119458546
5	23.257210454352588	33.698070432917625	29.39009686756408	13.654622245165704
6	21.983344778989284	42.81109428790516	25.15833431974377	10.047226613361788
7	91.80407837675475	2.767912986985066	4.412084141510317	1.0159244947498665
8	92.31521608319356	2.2692299993649083	4.108277964612289	1.3072759528292353
9	87.55187921690461	5.212398672583976	5.316706942690254	1.9190151678211582
10	39.87210189936933	37.8303187889071	11.721224842518236	10.576354469205338
11	29.50616792766576	26.216417049225054	27.038749743924555	17.23866527918463
12	29.957231385199496	24.52615849272202	27.335611920846308	18.180998201232175
13	24.403044585669313	29.389800326639826	27.928644345866555	18.278510741824306
14	19.056930667990557	32.413825825205436	29.093481808079208	19.4357616987248
15	18.219474386154108	29.03516209297596	35.45907871159888	17.286284809271056
16	22.543732990597924	27.34003532296643	33.50991521647858	16.60631646995707
17	22.777975609014746	26.390437148274664	27.879344417209357	22.952242825501234
18	23.042514825192832	27.45089220514998	30.496293362005545	19.010299607651646
19	25.02348233443935	28.683662250760445	27.34497767170399	18.94787774309622
20	26.256202956562436	29.002592014795415	27.048288476988052	17.692916551654093
21	24.743646548918502	27.8437595062989	29.717749877120852	17.69484406766174
22	24.079641996026847	25.15331783577514	29.514742902725434	21.252297265472578
23	20.85216471167944	29.228580910820508	29.50433925863286	20.414915118867192
24	20.819471074780456	28.596652201235635	32.06037375523858	18.52350296874533
25	21.259142418474102	29.727140339722226	29.408284710918313	19.605432530885356
26	20.87502307459067	31.480265572167067	27.219565572488317	20.42514578075395
27	19.54713752752579	31.870908816384087	29.40346592089919	19.178487735190934
28	18.13305741847781	30.37817122717778	31.723997500160007	19.764773854184405
29	19.47824118612416	28.397524970599203	31.789557756163788	20.33467608711285
30	21.12409273922018	29.05821814983669	30.477734842495995	19.339954268447134
31	23.643603278655306	28.866059630920223	27.9993446445574	19.49099244586707
32	24.242690080879065	28.783374136540786	28.18154433076768	18.79239145181247
33	23.357391863263004	28.904931203741157	27.789022994030383	19.948653938965453
34	20.424182022750127	29.359503728878565	30.107898886464117	20.10841536190719
35	20.25675995926516	28.987097751503153	30.04389547031267	20.712246818919013
36	23.1531245899395	29.563943984407874	28.214312102897726	19.068619322754895
37	21.391918617308747	30.616565418534154	28.804428542162807	19.187087421994292
38	20.86143161556237	30.480897945688035	28.39779679977977	20.25987363896983
39	21.06918324474585	29.88148989079633	28.107013711805216	20.942313152652595
40	22.781459964874728	28.294007476290933	28.496693198018413	20.427839360815923
41	19.017638995526926	27.61591722949722	29.81686868105269	23.54957509392316
42	21.582643855091323	27.996799334957558	28.574881155046672	21.845675654904454
43	21.550345606091344	27.62958282375659	29.116216612272	21.70385495788007
44	21.087148682406895	29.63568217633361	28.239097981816602	21.03807115944289
45	20.082122066623356	30.657537489568554	27.320611892427806	21.939728551380284
46	21.095946063159758	30.42186159001784	27.764113556393067	20.718078790429338
47	20.61263378011342	29.264709480092094	29.1910684839024	20.931588255892084
48	21.506852937200787	28.3184226790545	29.666917820355014	20.5078065633897
49	21.8407085944232	27.626098467896604	29.214964740048522	21.318228197631676
50	19.905383675768086	29.57899343631376	29.246719330687366	21.268903557230793
51	19.79853009606196	30.254736067457127	28.039847192461732	21.906886644019178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1623.0
1	2913.0
2	4203.0
3	14597.0
4	24991.0
5	19406.0
6	13821.0
7	13433.0
8	13045.0
9	13889.0
10	14733.0
11	15957.0
12	17181.0
13	17132.5
14	17084.0
15	17381.0
16	17678.0
17	17104.0
18	16530.0
19	16795.5
20	17061.0
21	19833.0
22	22605.0
23	23361.5
24	24118.0
25	30784.5
26	47666.0
27	57881.0
28	66138.0
29	74395.0
30	90659.5
31	106924.0
32	119317.5
33	131711.0
34	145585.5
35	159460.0
36	171609.5
37	183759.0
38	195847.5
39	207936.0
40	238152.0
41	268368.0
42	285682.0
43	302996.0
44	315408.5
45	327821.0
46	326374.5
47	324928.0
48	324167.0
49	323406.0
50	303028.0
51	282650.0
52	262736.5
53	242823.0
54	223180.0
55	203537.0
56	187004.5
57	170472.0
58	152942.0
59	135412.0
60	121716.0
61	108020.0
62	90902.0
63	73784.0
64	61397.0
65	49010.0
66	38325.0
67	27640.0
68	22042.0
69	16444.0
70	12906.5
71	9369.0
72	8076.0
73	6783.0
74	5311.0
75	3228.5
76	2618.0
77	1969.0
78	1320.0
79	990.5
80	661.0
81	452.0
82	243.0
83	188.5
84	134.0
85	102.5
86	71.0
87	71.5
88	72.0
89	51.5
90	31.0
91	19.5
92	8.0
93	6.0
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4046659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.41977696278797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53445043379563	15.251215113194384
2	8.715682615772787	3.3851322495351193
3	2.995837436629181	1.745354845083274
4	1.5085580579620181	1.1718344408415584
5	0.8907946377571072	0.8649516592445262
6	0.5416632089892361	0.6311387224511386
7	0.417011291664533	0.5668786392562548
8	0.32285987624634965	0.5015893429549949
9	0.2511696220989043	0.43898922368896165
>10	2.9219920802308095	13.992408546212678
>50	1.547723178256295	22.092508401669672
>100	1.3442858752477542	37.78933340359553
>500	0.00653416831925811	0.808480271128806
>1k	0.0013068336638516218	0.406968315529445
>5k	0.0	0.0
>10k+	1.3068336638516218E-4	0.3532168256135455
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13918	0.3439380486470444	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4188	0.10349278256457982	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01591436293495449	0.0
2	0.0	0.0	0.0	0.08330328797163289	0.0
3	0.0	0.0	0.0	0.12978607784841767	0.0
4	0.0	0.0	0.0	0.24271874650174377	0.0
5	0.0	0.0	0.0	0.4695725535559087	0.0
6	0.0	0.0	0.0	0.716689990434084	0.0
7	0.0	0.0	0.0	0.8459076981776819	0.0
8	0.0	0.0	0.0	1.1616002237895509	0.0
9	0.0	0.0	0.0	1.2563450490886432	0.0
10	0.0	0.0	0.0	1.45532400926295	0.0
11	0.0	0.0	0.0	1.760711737756999	0.0
12	0.0	0.0	0.0	1.9703908829481307	0.0
13	0.0	0.0	0.0	2.060588747408665	0.0
14	0.0	0.0	0.0	2.0977304981714546	0.0
15	0.0	0.0	0.0	2.162401131402473	0.0
16	0.0	0.0	0.0	2.3332581272600432	0.0
17	0.0	0.0	0.0	2.5470641336470408	0.0
18	2.471174368781753E-5	0.0	0.0	2.8054995491342365	0.0
19	2.471174368781753E-5	0.0	0.0	2.9485311215993244	0.0
20	2.471174368781753E-5	0.0	0.0	3.101496815026915	0.0
21	2.471174368781753E-5	0.0	0.0	3.31340001714995	0.0
22	2.471174368781753E-5	0.0	0.0	3.558268685352534	0.0
23	2.471174368781753E-5	0.0	0.0	3.8293565136078924	0.0
24	2.471174368781753E-5	0.0	0.0	4.023788512943641	0.0
25	2.471174368781753E-5	0.0	0.0	4.194447814851708	0.0
26	2.471174368781753E-5	0.0	0.0	4.362710077622058	0.0
27	2.471174368781753E-5	0.0	0.0	4.557809294037377	0.0
28	2.471174368781753E-5	0.0	0.0	4.7483368378704505	0.0
29	2.471174368781753E-5	0.0	0.0	4.973361975891717	0.0
30	2.471174368781753E-5	0.0	0.0	5.2318221031226	0.0
31	2.471174368781753E-5	0.0	0.0	5.464903269586095	0.0
32	2.471174368781753E-5	0.0	0.0	5.681724108703007	0.0
33	2.471174368781753E-5	0.0	0.0	5.909195709349367	0.0
34	2.471174368781753E-5	0.0	0.0	6.159797502087525	0.0
35	2.471174368781753E-5	0.0	0.0	6.446503152353584	0.0
36	2.471174368781753E-5	0.0	0.0	6.704938567840779	0.0
37	2.471174368781753E-5	0.0	0.0	6.969403648787803	0.0
38	2.471174368781753E-5	0.0	0.0	7.256702380902369	0.0
39	2.471174368781753E-5	0.0	0.0	7.655203959612114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	330	0.0	42.272728	31
GCGTAAG	290	0.0	41.12069	1
CGACGGT	340	0.0	41.02941	28
ACGGTAC	55	6.184564E-11	40.909092	20
TACGGGA	1575	0.0	40.57143	4
CGTAAGG	680	0.0	40.036762	2
CGTTTTT	8995	0.0	39.571987	1
ACGGGAC	1375	0.0	38.945454	5
AGGGCGA	3465	0.0	38.896107	6
TAGGGAC	5130	0.0	38.859646	5
CGAATAT	275	0.0	38.454544	14
TAGGGCG	1270	0.0	38.267715	5
CGTTAGG	730	0.0	38.219177	2
TAGGGAT	7000	0.0	38.153572	5
ACGGGAT	2115	0.0	38.085106	5
GTAGGGA	5920	0.0	37.550674	4
CGGACCG	90	0.0	37.5	6
CGGGTAT	265	0.0	37.35849	6
TAAGGGA	6320	0.0	37.34573	4
CGGGATT	2080	0.0	37.211536	6
>>END_MODULE